Ghent University Academic Bibliography

Advanced

Detection of metals in proteins by means of polyacrylamide gel electrophoresis and laser ablation-inductively coupled plasma-mass spectrometry: application to selenium

Cyrille C Chéry, Detlef Günther, Rita Cornelis UGent, Frank Vanhaecke UGent and Luc Moens UGent (2003) ELECTROPHORESIS. 24(19-20). p.3305-3313
abstract
The capabilities of laser ablation-inductively coupled plasma-mass spectrometry for the detection of trace elements in a gel after gel electrophoresis were systematically studied. Figures of merit, such as limit of detection, linearity, and repeatability, were evaluated for various elements (Li, V, Cr, Mn, Ni, Cu, Zn, As, Se, Mo, Pd, Ag, Cd, Pt, Tl, Pb). Two ablation strategies were followed: single hole drilling, relevant for ablation of spots after two-dimensional (2-D) separations, and ablation with translation, i.e., on a line, relevant for one-dimensional (1-D) separations. This technique was applied to the detection of selenoproteins in red blood cells extracts after a 1-D separation (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and the detection of selenium-containing proteins in yeast after 2-D electrophoresis (2-DE). The detection procedure was further improved by using the dynamic reaction cell technology, which allowed the removal of the Ar-2(+) interference and hence the use of the most abundant Se isotope, Se-80. Reaction gases were compared (methane, carbon monoxide, ammonia, oxygen and the combination of argon (collision gas) and hydrogen (reaction gas)). In each instance, the reaction cell parameters were optimized in order to obtain the lowest detection limit for Se (as Se-80(+), Se-82(+) or Se-77(+); and as Se-80 O-16(+), (SeO+)-Se-82-O-16 or (SeO+)-Se-77-O-16 with O-2 as the reaction gas). Carbon monoxide was found to offer the best performance. The detection limit with the use of DRC and He as transport gas was 0.07 mug Se g(-1) gel with single hole drilling and 0.15 mug Se g(-1) gel for ablation with translation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
metal-protein complexes, inductively coupled plasma-mass spectrometry laser ablation, selenium, ICP-MS, SPECIATION, YEAST
journal title
ELECTROPHORESIS
Electrophoresis
volume
24
issue
19-20
pages
3305 - 3313
Web of Science type
Article
Web of Science id
000186457900002
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
4.04 (2003)
JCR rank
2/67 (2003)
JCR quartile
1 (2003)
ISSN
0173-0835
DOI
10.1002/elps.200305590
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
211470
handle
http://hdl.handle.net/1854/LU-211470
date created
2004-04-19 10:35:00
date last changed
2012-11-13 16:42:56
@article{211470,
  abstract     = {The capabilities of laser ablation-inductively coupled plasma-mass spectrometry for the detection of trace elements in a gel after gel electrophoresis were systematically studied. Figures of merit, such as limit of detection, linearity, and repeatability, were evaluated for various elements (Li, V, Cr, Mn, Ni, Cu, Zn, As, Se, Mo, Pd, Ag, Cd, Pt, Tl, Pb). Two ablation strategies were followed: single hole drilling, relevant for ablation of spots after two-dimensional (2-D) separations, and ablation with translation, i.e., on a line, relevant for one-dimensional (1-D) separations. This technique was applied to the detection of selenoproteins in red blood cells extracts after a 1-D separation (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and the detection of selenium-containing proteins in yeast after 2-D electrophoresis (2-DE). The detection procedure was further improved by using the dynamic reaction cell technology, which allowed the removal of the Ar-2(+) interference and hence the use of the most abundant Se isotope, Se-80. Reaction gases were compared (methane, carbon monoxide, ammonia, oxygen and the combination of argon (collision gas) and hydrogen (reaction gas)). In each instance, the reaction cell parameters were optimized in order to obtain the lowest detection limit for Se (as Se-80(+), Se-82(+) or Se-77(+); and as Se-80 O-16(+), (SeO+)-Se-82-O-16 or (SeO+)-Se-77-O-16 with O-2 as the reaction gas). Carbon monoxide was found to offer the best performance. The detection limit with the use of DRC and He as transport gas was 0.07 mug Se g(-1) gel with single hole drilling and 0.15 mug Se g(-1) gel for ablation with translation.},
  author       = {Ch{\'e}ry, Cyrille C and G{\"u}nther, Detlef and Cornelis, Rita and Vanhaecke, Frank and Moens, Luc},
  issn         = {0173-0835},
  journal      = {ELECTROPHORESIS},
  keyword      = {metal-protein complexes,inductively coupled plasma-mass spectrometry laser ablation,selenium,ICP-MS,SPECIATION,YEAST},
  language     = {eng},
  number       = {19-20},
  pages        = {3305--3313},
  title        = {Detection of metals in proteins by means of polyacrylamide gel electrophoresis and laser ablation-inductively coupled plasma-mass spectrometry: application to selenium},
  url          = {http://dx.doi.org/10.1002/elps.200305590},
  volume       = {24},
  year         = {2003},
}

Chicago
Chéry, Cyrille C, Detlef Günther, Rita Cornelis, Frank Vanhaecke, and Luc Moens. 2003. “Detection of Metals in Proteins by Means of Polyacrylamide Gel Electrophoresis and Laser Ablation-inductively Coupled Plasma-mass Spectrometry: Application to Selenium.” Electrophoresis 24 (19-20): 3305–3313.
APA
Chéry, C. C., Günther, D., Cornelis, R., Vanhaecke, F., & Moens, L. (2003). Detection of metals in proteins by means of polyacrylamide gel electrophoresis and laser ablation-inductively coupled plasma-mass spectrometry: application to selenium. ELECTROPHORESIS, 24(19-20), 3305–3313.
Vancouver
1.
Chéry CC, Günther D, Cornelis R, Vanhaecke F, Moens L. Detection of metals in proteins by means of polyacrylamide gel electrophoresis and laser ablation-inductively coupled plasma-mass spectrometry: application to selenium. ELECTROPHORESIS. 2003;24(19-20):3305–13.
MLA
Chéry, Cyrille C, Detlef Günther, Rita Cornelis, et al. “Detection of Metals in Proteins by Means of Polyacrylamide Gel Electrophoresis and Laser Ablation-inductively Coupled Plasma-mass Spectrometry: Application to Selenium.” ELECTROPHORESIS 24.19-20 (2003): 3305–3313. Print.