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Synergistic degradation of linuron by a bacterial consortium and isolation of a single linuron-degrading Variovorax strain

Winnie DEJONGHE, Ellen BERTELOOT, Johan GORIS, Nico Boon UGent, Katrien Crul, Siska Maertens UGent, Monica Höfte UGent, Paul De Vos UGent, Willy Verstraete UGent and Eva TOP (2003) APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 69(3). p.1532-1541
abstract
The bacterial community composition of a linuron-degrading enrichment culture and the role of the individual strains in linuron degradation have been determined by a combination of methods, such as denaturing gradient gel electrophoresis of the total 16S rRNA gene pool, isolation and identification of strains, and biodegradation assays. Three strains, Variovorax sp. strain WDL1, Delftia acidovorans WDL34, and Pseudomonas sp. strain WDL5, were isolated directly from the linuron-degrading culture. In addition, subculture of this enrichment culture on potential intermediates in the degradation pathway of linuron (i.e., N,O-dimethylhydroxylamine and 3-chloroaniline) resulted in the isolation of, respectively, Hyphomicrobium sulfionivorans WDL6 and Comamonas testosteroni WDL7. Of these five strains, only Variovorax sp. strain WDL1 was able to use linuron as the sole source of C, N, and energy. WDL1 first converted linuron to 3,4-dichloroaniline (3,4-DCA), which transiently accumulated in the medium but was subsequently degraded. To the best of our knowledge, this is the first report of a strain that degrades linuron further than the aromatic intermediates. Interestingly, the rate of linuron degradation by strain WDL1 was lower than that for the consortium, but was clearly increased when WDL1 was coinoculated with each of the other four strains. D. acidovorans WDL34 and C. testosteroni WDL7 were found to be responsible for degradation of the intermediate 3,4-DCA, and H. sulfonivorans WDL6 was the only strain able to degrade NO-dimethylhydroxylamine. The role of Pseudomonas sp. strain WDL5 needs to be further elucidated. The degradation of linuron can thus be performed by a single isolate, Variovorax sp. strain WDL1, but is stimulated by a synergistic interaction with the other strains isolated from the same linuron-degrading culture.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Appl. Environ. Microbiol.
volume
69
issue
3
pages
1532-1541 pages
Web of Science type
Article
Web of Science id
000181435600027
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.82 (2003)
JCR rank
15/132 (2003)
JCR quartile
1 (2003)
ISSN
0099-2240
language
English
UGent publication?
yes
classification
A1
id
211356
handle
http://hdl.handle.net/1854/LU-211356
date created
2004-04-09 11:41:00
date last changed
2010-03-04 13:30:07
@article{211356,
  abstract     = {The bacterial community composition of a linuron-degrading enrichment culture and the role of the individual strains in linuron degradation have been determined by a combination of methods, such as denaturing gradient gel electrophoresis of the total 16S rRNA gene pool, isolation and identification of strains, and biodegradation assays. Three strains, Variovorax sp. strain WDL1, Delftia acidovorans WDL34, and Pseudomonas sp. strain WDL5, were isolated directly from the linuron-degrading culture. In addition, subculture of this enrichment culture on potential intermediates in the degradation pathway of linuron (i.e., N,O-dimethylhydroxylamine and 3-chloroaniline) resulted in the isolation of, respectively, Hyphomicrobium sulfionivorans WDL6 and Comamonas testosteroni WDL7. Of these five strains, only Variovorax sp. strain WDL1 was able to use linuron as the sole source of C, N, and energy. WDL1 first converted linuron to 3,4-dichloroaniline (3,4-DCA), which transiently accumulated in the medium but was subsequently degraded. To the best of our knowledge, this is the first report of a strain that degrades linuron further than the aromatic intermediates. Interestingly, the rate of linuron degradation by strain WDL1 was lower than that for the consortium, but was clearly increased when WDL1 was coinoculated with each of the other four strains. D. acidovorans WDL34 and C. testosteroni WDL7 were found to be responsible for degradation of the intermediate 3,4-DCA, and H. sulfonivorans WDL6 was the only strain able to degrade NO-dimethylhydroxylamine. The role of Pseudomonas sp. strain WDL5 needs to be further elucidated. The degradation of linuron can thus be performed by a single isolate, Variovorax sp. strain WDL1, but is stimulated by a synergistic interaction with the other strains isolated from the same linuron-degrading culture.},
  author       = {DEJONGHE, Winnie and BERTELOOT, Ellen and GORIS, Johan and Boon, Nico and Crul, Katrien and Maertens, Siska and H{\"o}fte, Monica and De Vos, Paul and Verstraete, Willy and TOP, Eva},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  language     = {eng},
  number       = {3},
  pages        = {1532--1541},
  title        = {Synergistic degradation of linuron by a bacterial consortium and isolation of a single linuron-degrading Variovorax strain},
  volume       = {69},
  year         = {2003},
}

Chicago
DEJONGHE, Winnie, Ellen BERTELOOT, Johan GORIS, Nico Boon, Katrien Crul, Siska Maertens, Monica Höfte, Paul De Vos, Willy Verstraete, and Eva TOP. 2003. “Synergistic Degradation of Linuron by a Bacterial Consortium and Isolation of a Single Linuron-degrading Variovorax Strain.” Applied and Environmental Microbiology 69 (3): 1532–1541.
APA
DEJONGHE, Winnie, BERTELOOT, E., GORIS, J., Boon, N., Crul, K., Maertens, S., Höfte, M., et al. (2003). Synergistic degradation of linuron by a bacterial consortium and isolation of a single linuron-degrading Variovorax strain. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(3), 1532–1541.
Vancouver
1.
DEJONGHE W, BERTELOOT E, GORIS J, Boon N, Crul K, Maertens S, et al. Synergistic degradation of linuron by a bacterial consortium and isolation of a single linuron-degrading Variovorax strain. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2003;69(3):1532–41.
MLA
DEJONGHE, Winnie, Ellen BERTELOOT, Johan GORIS, et al. “Synergistic Degradation of Linuron by a Bacterial Consortium and Isolation of a Single Linuron-degrading Variovorax Strain.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 69.3 (2003): 1532–1541. Print.