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X-irradiation induces cell death in fetal fibroblasts

Michaël Beck UGent, Marjan Moreels, Paul Jacquet, Patric Van Oostveldt UGent, Winnok De Vos UGent and Sarah Baatout UGent (2012) INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE. 30(1). p.114-118
abstract
The impact of ionizing radiation on developing organisms has been widely studied for risk assessment purposes. Even though efforts have been made to decrease received doses to as low as reasonably achievable, the possibility of accidental exposure has to be considered as well. Mammalian gestation is usually divided into three periods. Radiation exposure during the 'pre-implantation period' may essentially result in embryonic mortality while exposure during the 'organogenesis period' may characteristically lead to malformations. In humans, the 'fetal period' is one of particular sensitivity to radiation induction of mental retardation, especially if the exposure occurs between weeks 8-15 of gestation. It is also admitted that prenatal irradiation may increase the risk of leukemia and childhood cancer, with an equal risk over the whole pregnancy. The aim of this study was to investigate the effects of moderate to high doses of X-irradiation on mouse skin fetal fibroblasts, one of the cell types subjected to the highest dose of radiation. Exposure of the cells to X-rays led to a rapid and significant increase in γ-H2AX foci, indicative of high levels of DNA double strand breaks. High doses (>2 Gy) also led to a pronounced G2-arrest and a decrease in the number of cells in the S phase, which was followed after 24 h by a decrease in cell survival and an increase in the level of apoptosis and necrosis. This study shows that mouse fetal skin fibroblasts are sensitive to high doses of X-irradiation. Furthermore, we report a better repair for higher doses than lower, which seems to indicate that little DNA damage is not necessarily repaired immediately. However, more sensitive approaches are necessary to identify the risk associated with low doses of radiation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IONIZING-RADIATION, DNA-DAMAGE, RADIOSENSITIVITY, PHOSPHORYLATION, APOPTOSIS, HISTONE H2AX, DNA damage, gamma-H2AX, cell cycle arrest, apoptosis, DOUBLE-STRAND BREAKS, fetal fibroblasts, X-rays
journal title
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Int. J. Mol. Med.
volume
30
issue
1
pages
114 - 118
Web of Science type
Article
Web of Science id
000304579600017
JCR category
MEDICINE, RESEARCH & EXPERIMENTAL
JCR impact factor
1.957 (2012)
JCR rank
73/119 (2012)
JCR quartile
3 (2012)
ISSN
1107-3756
DOI
10.3892/ijmm.2012.964
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2112401
handle
http://hdl.handle.net/1854/LU-2112401
date created
2012-05-24 15:37:21
date last changed
2012-09-28 16:11:35
@article{2112401,
  abstract     = {The impact of ionizing radiation on developing organisms has been widely studied for risk assessment purposes. Even though efforts have been made to decrease received doses to as low as reasonably achievable, the possibility of accidental exposure has to be considered as well. Mammalian gestation is usually divided into three periods. Radiation exposure during the 'pre-implantation period' may essentially result in embryonic mortality while exposure during the 'organogenesis period' may characteristically lead to malformations. In humans, the 'fetal period' is one of particular sensitivity to radiation induction of mental retardation, especially if the exposure occurs between weeks 8-15 of gestation. It is also admitted that prenatal irradiation may increase the risk of leukemia and childhood cancer, with an equal risk over the whole pregnancy. The aim of this study was to investigate the effects of moderate to high doses of X-irradiation on mouse skin fetal fibroblasts, one of the cell types subjected to the highest dose of radiation. Exposure of the cells to X-rays led to a rapid and significant increase in \ensuremath{\gamma}-H2AX foci, indicative of high levels of DNA double strand breaks. High doses ({\textrangle}2 Gy) also led to a pronounced G2-arrest and a decrease in the number of cells in the S phase, which was followed after 24 h by a decrease in cell survival and an increase in the level of apoptosis and necrosis. This study shows that mouse fetal skin fibroblasts are sensitive to high doses of X-irradiation. Furthermore, we report a better repair for higher doses than lower, which seems to indicate that little DNA damage is not necessarily repaired immediately. However, more sensitive approaches are necessary to identify the risk associated with low doses of radiation.},
  author       = {Beck, Micha{\"e}l and Moreels, Marjan and Jacquet, Paul and Van Oostveldt, Patric and De Vos, Winnok and Baatout, Sarah},
  issn         = {1107-3756},
  journal      = {INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE},
  keyword      = {IONIZING-RADIATION,DNA-DAMAGE,RADIOSENSITIVITY,PHOSPHORYLATION,APOPTOSIS,HISTONE H2AX,DNA damage,gamma-H2AX,cell cycle arrest,apoptosis,DOUBLE-STRAND BREAKS,fetal fibroblasts,X-rays},
  language     = {eng},
  number       = {1},
  pages        = {114--118},
  title        = {X-irradiation induces cell death in fetal fibroblasts},
  url          = {http://dx.doi.org/10.3892/ijmm.2012.964},
  volume       = {30},
  year         = {2012},
}

Chicago
Beck, Michaël, Marjan Moreels, Paul Jacquet, Patric Van Oostveldt, Winnok De Vos, and Sarah Baatout. 2012. “X-irradiation Induces Cell Death in Fetal Fibroblasts.” International Journal of Molecular Medicine 30 (1): 114–118.
APA
Beck, Michaël, Moreels, M., Jacquet, P., Van Oostveldt, P., De Vos, W., & Baatout, S. (2012). X-irradiation induces cell death in fetal fibroblasts. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 30(1), 114–118.
Vancouver
1.
Beck M, Moreels M, Jacquet P, Van Oostveldt P, De Vos W, Baatout S. X-irradiation induces cell death in fetal fibroblasts. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE. 2012;30(1):114–8.
MLA
Beck, Michaël, Marjan Moreels, Paul Jacquet, et al. “X-irradiation Induces Cell Death in Fetal Fibroblasts.” INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 30.1 (2012): 114–118. Print.