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Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica

(2012) POLAR BIOLOGY. 35(4). p.543-554
Author
Organization
Project
AMBIO
Project
BELDIVA
Project
ANTAR-IMPACT
Abstract
Heterotrophic bacteria isolated from five aquatic microbial mat samples from different locations in continental Antarctica and the Antarctic Peninsula were compared to assess their biodiversity. A total of 2,225 isolates obtained on different media and at different temperatures were included. After an initial grouping by whole-genome fingerprinting, partial 16S rRNA gene sequence analysis was used for further identification. These results were compared with previously published data obtained with the same methodology from terrestrial and aquatic microbial mat samples from two additional Antarctic regions. The phylotypes recovered in all these samples belonged to five major phyla, Actinobacteria, Bacteroidetes, Proteobacteria, Firmicutes and Deinococcus-Thermus, and included several potentially new taxa. Ordination analyses were performed in order to explore the variance in the diversity of the samples at genus level. Habitat type (terrestrial vs. aquatic) and specific conductivity in the lacustrine systems significantly explained the variation in bacterial community structure. Comparison of the phylotypes with sequences from public databases showed that a considerable proportion (36.9%) is currently known only from Antarctica. This suggests that in Antarctica, both cosmopolitan taxa and taxa with limited dispersal and a history of long-term isolated evolution occur.
Keywords
Cultivation, Microbial diversity, 16S rRNA gene sequencing, ASPA, PCA, MCMURDO DRY VALLEYS, SP-NOV., GEN.-NOV., FRESH-WATER, SP. NOV., EMENDED DESCRIPTION, SCHIRMACHER OASIS, EAST ANTARCTICA, VICTORIA LAND, LAKE FRYXELL

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Citation

Please use this url to cite or link to this publication:

Chicago
Peeters, Karolien, Elie Verleyen, Dominic A Hodgson, Peter Convey, Damien Ertz, Wim Vyverman, and Anne Willems. 2012. “Heterotrophic Bacterial Diversity in Aquatic Microbial Mat Communities from Antarctica.” Polar Biology 35 (4): 543–554.
APA
Peeters, Karolien, Verleyen, E., Hodgson, D. A., Convey, P., Ertz, D., Vyverman, W., & Willems, A. (2012). Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica. POLAR BIOLOGY, 35(4), 543–554.
Vancouver
1.
Peeters K, Verleyen E, Hodgson DA, Convey P, Ertz D, Vyverman W, et al. Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica. POLAR BIOLOGY. 2012;35(4):543–54.
MLA
Peeters, Karolien, Elie Verleyen, Dominic A Hodgson, et al. “Heterotrophic Bacterial Diversity in Aquatic Microbial Mat Communities from Antarctica.” POLAR BIOLOGY 35.4 (2012): 543–554. Print.
@article{2112140,
  abstract     = {Heterotrophic bacteria isolated from five aquatic microbial mat samples from different locations in continental Antarctica and the Antarctic Peninsula were compared to assess their biodiversity. A total of 2,225 isolates obtained on different media and at different temperatures were included. After an initial grouping by whole-genome fingerprinting, partial 16S rRNA gene sequence analysis was used for further identification. These results were compared with previously published data obtained with the same methodology from terrestrial and aquatic microbial mat samples from two additional Antarctic regions. The phylotypes recovered in all these samples belonged to five major phyla, Actinobacteria, Bacteroidetes, Proteobacteria, Firmicutes and Deinococcus-Thermus, and included several potentially new taxa. Ordination analyses were performed in order to explore the variance in the diversity of the samples at genus level. Habitat type (terrestrial vs. aquatic) and specific conductivity in the lacustrine systems significantly explained the variation in bacterial community structure. Comparison of the phylotypes with sequences from public databases showed that a considerable proportion (36.9\%) is currently known only from Antarctica. This suggests that in Antarctica, both cosmopolitan taxa and taxa with limited dispersal and a history of long-term isolated evolution occur.},
  author       = {Peeters, Karolien and Verleyen, Elie and Hodgson, Dominic A and Convey, Peter and Ertz, Damien and Vyverman, Wim and Willems, Anne},
  issn         = {0722-4060},
  journal      = {POLAR BIOLOGY},
  keyword      = {Cultivation,Microbial diversity,16S rRNA gene sequencing,ASPA,PCA,MCMURDO DRY VALLEYS,SP-NOV.,GEN.-NOV.,FRESH-WATER,SP. NOV.,EMENDED DESCRIPTION,SCHIRMACHER OASIS,EAST ANTARCTICA,VICTORIA LAND,LAKE FRYXELL},
  language     = {eng},
  number       = {4},
  pages        = {543--554},
  title        = {Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica},
  url          = {http://dx.doi.org/10.1007/s00300-011-1100-4},
  volume       = {35},
  year         = {2012},
}

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