Advanced search
1 file | 1.13 MB

Determining milk isolated and conjugated trans-unsaturated fatty acids using Fourier transform Raman spectroscopy

Author
Organization
Abstract
The feasibility of Raman spectroscopy in combination with partial least-squares (PLS) regression for the determination of individual or grouped trans-monounsaturated fatty acids (trans-MUFA) and conjugated linoleic acids (CLA) in milk fat is demonstrated using spectra obtained at two temperature conditions: room, temperature and after freezing at -80 degrees C. The PLS results displayed capability for direct semiroutine quantification of several individual CLA (cis-9,trans-11 and trans-10,cis-12 C18:2) and trans-MUFA (trans-4-15 C18:1) in minor concentrations (below 1.0 g/100 g of milk fat). Calibration models were based on reference data cross-correlation or determined by specific scattering signals in the Raman spectra. Distinct bands for trans-MUFA (1674 cm(-1)) and CLA (1653 cm(-1)) from the trans isolated and cis,trans conjugated C=C bonds were identified, as well as original evidence for the temperature effect (new bands, peak shifts, and higher intensities) on the Raman spectra of fatty acid methyl ester and triacylglyceride standards, are supplied.
Keywords
HUMANS, SUPPLEMENTATION, COWS, ODD, ISOMERS, RAPID-DETERMINATION, trans-fatty acids, LINOLEIC-ACID, PLS, milk fat, CLA, REFLECTION INFRARED-SPECTROSCOPY, spectroscopy, FT-Raman, OILS, MSC

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.13 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Stefanov, Ivan, Vincent Baeten, Ouissam Abbas, Ellen Colman, Bruno Vlaeminck, Bernard De Baets, and Veerle Fievez. 2011. “Determining Milk Isolated and Conjugated Trans-unsaturated Fatty Acids Using Fourier Transform Raman Spectroscopy.” Journal of Agricultural and Food Chemistry 59 (24): 12771–12783.
APA
Stefanov, I., Baeten, V., Abbas, O., Colman, E., Vlaeminck, B., De Baets, B., & Fievez, V. (2011). Determining milk isolated and conjugated trans-unsaturated fatty acids using Fourier transform Raman spectroscopy. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59(24), 12771–12783.
Vancouver
1.
Stefanov I, Baeten V, Abbas O, Colman E, Vlaeminck B, De Baets B, et al. Determining milk isolated and conjugated trans-unsaturated fatty acids using Fourier transform Raman spectroscopy. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2011;59(24):12771–83.
MLA
Stefanov, Ivan, Vincent Baeten, Ouissam Abbas, et al. “Determining Milk Isolated and Conjugated Trans-unsaturated Fatty Acids Using Fourier Transform Raman Spectroscopy.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 59.24 (2011): 12771–12783. Print.
@article{2111824,
  abstract     = {The feasibility of Raman spectroscopy in combination with partial least-squares (PLS) regression for the determination of individual or grouped trans-monounsaturated fatty acids (trans-MUFA) and conjugated linoleic acids (CLA) in milk fat is demonstrated using spectra obtained at two temperature conditions: room, temperature and after freezing at -80 degrees C. The PLS results displayed capability for direct semiroutine quantification of several individual CLA (cis-9,trans-11 and trans-10,cis-12 C18:2) and trans-MUFA (trans-4-15 C18:1) in minor concentrations (below 1.0 g/100 g of milk fat). Calibration models were based on reference data cross-correlation or determined by specific scattering signals in the Raman spectra. Distinct bands for trans-MUFA (1674 cm(-1)) and CLA (1653 cm(-1)) from the trans isolated and cis,trans conjugated C=C bonds were identified, as well as original evidence for the temperature effect (new bands, peak shifts, and higher intensities) on the Raman spectra of fatty acid methyl ester and triacylglyceride standards, are supplied.},
  author       = {Stefanov, Ivan and Baeten, Vincent and Abbas, Ouissam and Colman, Ellen and Vlaeminck, Bruno and De Baets, Bernard and Fievez, Veerle},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keyword      = {HUMANS,SUPPLEMENTATION,COWS,ODD,ISOMERS,RAPID-DETERMINATION,trans-fatty acids,LINOLEIC-ACID,PLS,milk fat,CLA,REFLECTION INFRARED-SPECTROSCOPY,spectroscopy,FT-Raman,OILS,MSC},
  language     = {eng},
  number       = {24},
  pages        = {12771--12783},
  title        = {Determining milk isolated and conjugated trans-unsaturated fatty acids using Fourier transform Raman spectroscopy},
  url          = {http://dx.doi.org/10.1021/jf203463r},
  volume       = {59},
  year         = {2011},
}

Altmetric
View in Altmetric
Web of Science
Times cited: