Repression of early lateral root initiation events by transient water deficit in barley and maize
(2012)
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES.
367(1595).
p.1534-1541
- Author
- Aurélie Babé, Tristan Lavigne, Jean-Philippe Séverin, Kerstin A Nagel, Achim Walter, François Chaumont, Henri Batoko, Tom Beeckman (UGent) and Xavier Draye
- Organization
- Abstract
- The formation of lateral roots (LRs) is a key driver of root system architecture and developmental plasticity. The first stage of LR formation, which leads to the acquisition of founder cell identity in the pericycle, is the primary determinant of root branching patterns. The fact that initiation events occur asynchronously in a very small number of cells inside the parent root has been a major difficulty in the study of the molecular regulation of branching patterns. Inducible systems that trigger synchronous lateral formation at predictable sites have proven extremely valuable in Arabidopsis to decipher the first steps of LR formation. Here, we present a LR repression system for cereals that relies on a transient water-deficit treatment, which blocks LR initiation before the first formative divisions. Using a time-lapse approach, we analysed the dynamics of this repression along growing roots and were able to show that it targets a very narrow developmental window of the initiation process. Interestingly, the repression can be exploited to obtain negative control root samples where LR initiation is absent. This system could be instrumental in the analysis of the molecular basis of drought-responsive as well as intrinsic pathways of LR formation in cereals.
- Keywords
- ZEA-MAYS, AUXIN TRANSPORT, SYSTEM ARCHITECTURE, ARABIDOPSIS-THALIANA, maize, barley, water deficit, lateral root initiation, DYNAMICS, RESPONSES, QUANTITATIVE-ANALYSIS, ABSCISIC-ACID, GROWTH, MERISTEM
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-2111585
- MLA
- Babé, Aurélie, et al. “Repression of Early Lateral Root Initiation Events by Transient Water Deficit in Barley and Maize.” PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, vol. 367, no. 1595, 2012, pp. 1534–41, doi:10.1098/rstb.2011.0240.
- APA
- Babé, A., Lavigne, T., Séverin, J.-P., Nagel, K. A., Walter, A., Chaumont, F., … Draye, X. (2012). Repression of early lateral root initiation events by transient water deficit in barley and maize. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 367(1595), 1534–1541. https://doi.org/10.1098/rstb.2011.0240
- Chicago author-date
- Babé, Aurélie, Tristan Lavigne, Jean-Philippe Séverin, Kerstin A Nagel, Achim Walter, François Chaumont, Henri Batoko, Tom Beeckman, and Xavier Draye. 2012. “Repression of Early Lateral Root Initiation Events by Transient Water Deficit in Barley and Maize.” PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 367 (1595): 1534–41. https://doi.org/10.1098/rstb.2011.0240.
- Chicago author-date (all authors)
- Babé, Aurélie, Tristan Lavigne, Jean-Philippe Séverin, Kerstin A Nagel, Achim Walter, François Chaumont, Henri Batoko, Tom Beeckman, and Xavier Draye. 2012. “Repression of Early Lateral Root Initiation Events by Transient Water Deficit in Barley and Maize.” PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 367 (1595): 1534–1541. doi:10.1098/rstb.2011.0240.
- Vancouver
- 1.Babé A, Lavigne T, Séverin J-P, Nagel KA, Walter A, Chaumont F, et al. Repression of early lateral root initiation events by transient water deficit in barley and maize. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES. 2012;367(1595):1534–41.
- IEEE
- [1]A. Babé et al., “Repression of early lateral root initiation events by transient water deficit in barley and maize,” PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, vol. 367, no. 1595, pp. 1534–1541, 2012.
@article{2111585,
abstract = {{The formation of lateral roots (LRs) is a key driver of root system architecture and developmental plasticity. The first stage of LR formation, which leads to the acquisition of founder cell identity in the pericycle, is the primary determinant of root branching patterns. The fact that initiation events occur asynchronously in a very small number of cells inside the parent root has been a major difficulty in the study of the molecular regulation of branching patterns. Inducible systems that trigger synchronous lateral formation at predictable sites have proven extremely valuable in Arabidopsis to decipher the first steps of LR formation. Here, we present a LR repression system for cereals that relies on a transient water-deficit treatment, which blocks LR initiation before the first formative divisions. Using a time-lapse approach, we analysed the dynamics of this repression along growing roots and were able to show that it targets a very narrow developmental window of the initiation process. Interestingly, the repression can be exploited to obtain negative control root samples where LR initiation is absent. This system could be instrumental in the analysis of the molecular basis of drought-responsive as well as intrinsic pathways of LR formation in cereals.}},
author = {{Babé, Aurélie and Lavigne, Tristan and Séverin, Jean-Philippe and Nagel, Kerstin A and Walter, Achim and Chaumont, François and Batoko, Henri and Beeckman, Tom and Draye, Xavier}},
issn = {{0962-8436}},
journal = {{PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES}},
keywords = {{ZEA-MAYS,AUXIN TRANSPORT,SYSTEM ARCHITECTURE,ARABIDOPSIS-THALIANA,maize,barley,water deficit,lateral root initiation,DYNAMICS,RESPONSES,QUANTITATIVE-ANALYSIS,ABSCISIC-ACID,GROWTH,MERISTEM}},
language = {{eng}},
number = {{1595}},
pages = {{1534--1541}},
title = {{Repression of early lateral root initiation events by transient water deficit in barley and maize}},
url = {{http://doi.org/10.1098/rstb.2011.0240}},
volume = {{367}},
year = {{2012}},
}
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