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Tissue culture of Hypericum brasiliense Choisy: shoot multiplication and callus induction

Author
Organization
Abstract
Hypericum brasiliense, anon-domesticated plant has been shown to have useful medicinal properties. This plant has not been cultivated so a protocol for mass propagation based on selection of superior clones was developed and a protocol established for the culture of callus cells that could be used for in vitro metabolite production. A micropropagation method based on amplification of nodal buds was developed, by selection, from ten seedling clones that were examined for growth rate, multiplication rate and rooting. The effect of various basal media, growth regulator types and concentrations were examined for optimal callus induction. Optimal callus induction occured on either Murashige and Skoog's or Gamborg's media supplemented with 1 to 2 mg 1(-1) of 2,4-dichlorophenoxyacetic acid.
Keywords
Hypericum brasiliense, cell culture, medicinal plant, micropropagation, ANTIVIRAL AGENT, FLAVONOIDS

Citation

Please use this url to cite or link to this publication:

Chicago
Cardoso, MA, and Dulce De Oliveira. 1996. “Tissue Culture of Hypericum Brasiliense Choisy: Shoot Multiplication and Callus Induction.” Plant Cell Tissue and Organ Culture 44 (2): 91–94.
APA
Cardoso, M., & De Oliveira, D. (1996). Tissue culture of Hypericum brasiliense Choisy: shoot multiplication and callus induction. PLANT CELL TISSUE AND ORGAN CULTURE, 44(2), 91–94.
Vancouver
1.
Cardoso M, De Oliveira D. Tissue culture of Hypericum brasiliense Choisy: shoot multiplication and callus induction. PLANT CELL TISSUE AND ORGAN CULTURE. 1996;44(2):91–4.
MLA
Cardoso, MA, and Dulce De Oliveira. “Tissue Culture of Hypericum Brasiliense Choisy: Shoot Multiplication and Callus Induction.” PLANT CELL TISSUE AND ORGAN CULTURE 44.2 (1996): 91–94. Print.
@article{2111337,
  abstract     = {Hypericum brasiliense, anon-domesticated plant has been shown to have useful medicinal properties. This plant has not been cultivated so a protocol for mass propagation based on selection of superior clones was developed and a protocol established for the culture of callus cells that could be used for in vitro metabolite production. A micropropagation method based on amplification of nodal buds was developed, by selection, from ten seedling clones that were examined for growth rate, multiplication rate and rooting. The effect of various basal media, growth regulator types and concentrations were examined for optimal callus induction. Optimal callus induction occured on either Murashige and Skoog's or Gamborg's media supplemented with 1 to 2 mg 1(-1) of 2,4-dichlorophenoxyacetic acid.},
  author       = {Cardoso, MA and De Oliveira, Dulce},
  issn         = {0167-6857},
  journal      = {PLANT CELL TISSUE AND ORGAN CULTURE},
  keyword      = {Hypericum brasiliense,cell culture,medicinal plant,micropropagation,ANTIVIRAL AGENT,FLAVONOIDS},
  language     = {eng},
  number       = {2},
  pages        = {91--94},
  title        = {Tissue culture of Hypericum brasiliense Choisy: shoot multiplication and callus induction},
  url          = {http://dx.doi.org/10.1007/BF00048184},
  volume       = {44},
  year         = {1996},
}

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