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Susceptibility of piglets to enterotoxigenic Escherichia coli is not related to the expression of MUC13 and MUC20

M Schroyen, A Stinckens, R Verhelst, M Geens, Eric Cox UGent, T Niewold and N Buys (2012) ANIMAL GENETICS. 43(3). p.324-327
abstract
Enterotoxigenic Escherichia coli (ETEC) is one of the most frequently isolated enteropathogens in production animals, especially pigs and calves. Economically, the swine industry is by far the most affected by infections with ETEC because of mortality, morbidity and decreased growth rate of newborn and early-weaned piglets. After ingestion by the animal, these bacteria attach themselves to specific receptors on the small intestinal epithelium by means of proteinaceous surface appendages, the fimbriae. The F4 fimbriae, which attach to the F4 receptor, are the most studied. The aim of our study was to investigate gene expression in the small intestine of piglets of MUC13 and MUC20 in relation to animals with a different treatment towards or a different reaction on ETEC-F4ac by means of quantitative reverse transcription chain reaction (qRT/PCR). MUC13 and MUC20 are positional candidate genes for this F4ac receptor and are located in the region on SSC13q41 that segregates with the susceptibility to ETEC-F4ac. The condition of the small intestine is crucial when examining expression differences between different samples. Therefore, the expression of two genes, fatty-acid binding protein 2, intestinal (FABP2) and pancreatitis-associated protein (PAP), now known as regenerating islet-derived 3 alpha (REG3A) in the small intestine was simultaneously checked. FABP2, a standard for epithelial content, reflects the state of damage, whereas REG3A is a measure for inflammation in the small intestine. The four different substudies presented here suggest that expression of MUC13 and MUC20 is not related to the susceptibility of piglets to ETEC-F4ac.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PCR, small intestine, qRT, pig, MUC20, MUC13, ETEC-F4ac, ANTIGEN, INTESTINE, GENE, PROTEIN
journal title
ANIMAL GENETICS
Anim. Genet.
volume
43
issue
3
pages
324 - 327
Web of Science type
Article
Web of Science id
000302541500012
JCR category
AGRICULTURE, DAIRY & ANIMAL SCIENCE
JCR impact factor
2.584 (2012)
JCR rank
2/54 (2012)
JCR quartile
1 (2012)
ISSN
0268-9146
DOI
10.1111/j.1365-2052.2011.02241.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2111201
handle
http://hdl.handle.net/1854/LU-2111201
date created
2012-05-22 16:08:34
date last changed
2012-05-23 09:20:41
@article{2111201,
  abstract     = {Enterotoxigenic Escherichia coli (ETEC) is one of the most frequently isolated enteropathogens in production animals, especially pigs and calves. Economically, the swine industry is by far the most affected by infections with ETEC because of mortality, morbidity and decreased growth rate of newborn and early-weaned piglets. After ingestion by the animal, these bacteria attach themselves to specific receptors on the small intestinal epithelium by means of proteinaceous surface appendages, the fimbriae. The F4 fimbriae, which attach to the F4 receptor, are the most studied. The aim of our study was to investigate gene expression in the small intestine of piglets of MUC13 and MUC20 in relation to animals with a different treatment towards or a different reaction on ETEC-F4ac by means of quantitative reverse transcription chain reaction (qRT/PCR). MUC13 and MUC20 are positional candidate genes for this F4ac receptor and are located in the region on SSC13q41 that segregates with the susceptibility to ETEC-F4ac. The condition of the small intestine is crucial when examining expression differences between different samples. Therefore, the expression of two genes, fatty-acid binding protein 2, intestinal (FABP2) and pancreatitis-associated protein (PAP), now known as regenerating islet-derived 3 alpha (REG3A) in the small intestine was simultaneously checked. FABP2, a standard for epithelial content, reflects the state of damage, whereas REG3A is a measure for inflammation in the small intestine. The four different substudies presented here suggest that expression of MUC13 and MUC20 is not related to the susceptibility of piglets to ETEC-F4ac.},
  author       = {Schroyen, M and Stinckens, A and Verhelst, R and Geens, M and Cox, Eric and Niewold, T and Buys, N},
  issn         = {0268-9146},
  journal      = {ANIMAL GENETICS},
  keyword      = {PCR,small intestine,qRT,pig,MUC20,MUC13,ETEC-F4ac,ANTIGEN,INTESTINE,GENE,PROTEIN},
  language     = {eng},
  number       = {3},
  pages        = {324--327},
  title        = {Susceptibility of piglets to enterotoxigenic Escherichia coli is not related to the expression of MUC13 and MUC20},
  url          = {http://dx.doi.org/10.1111/j.1365-2052.2011.02241.x},
  volume       = {43},
  year         = {2012},
}

Chicago
Schroyen, M, A Stinckens, R Verhelst, M Geens, Eric Cox, T Niewold, and N Buys. 2012. “Susceptibility of Piglets to Enterotoxigenic Escherichia Coli Is Not Related to the Expression of MUC13 and MUC20.” Animal Genetics 43 (3): 324–327.
APA
Schroyen, M., Stinckens, A., Verhelst, R., Geens, M., Cox, E., Niewold, T., & Buys, N. (2012). Susceptibility of piglets to enterotoxigenic Escherichia coli is not related to the expression of MUC13 and MUC20. ANIMAL GENETICS, 43(3), 324–327.
Vancouver
1.
Schroyen M, Stinckens A, Verhelst R, Geens M, Cox E, Niewold T, et al. Susceptibility of piglets to enterotoxigenic Escherichia coli is not related to the expression of MUC13 and MUC20. ANIMAL GENETICS. 2012;43(3):324–7.
MLA
Schroyen, M, A Stinckens, R Verhelst, et al. “Susceptibility of Piglets to Enterotoxigenic Escherichia Coli Is Not Related to the Expression of MUC13 and MUC20.” ANIMAL GENETICS 43.3 (2012): 324–327. Print.