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Regulation of transformation efficiency of peanut (Arachis hypogaea L.) explants by Agrobacterium tumefaciens

(1993) PLANT SCIENCE. 89(1). p.93-99
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Abstract
Some of the factors that modulate the transformation efficiency of regenerable tissues of peanut (Arachis hypogaea L.) were examined, using a tumor induction system based on strain A281 harbouring a binary vector. Factors examined were the composition of media, the cocultivation period, tissue type, bacterial inoculum concentration and pretreatment with acetosyringone (AS). Transformation efficiency was greatest when leaf or cotyledon explants were cocultivated on solid rather than liquid medium for 48 h. Highest transformation rates were observed on leaves from 7-10-day-old seedlings, with inoculum densities of 10(9)-5 x 10(9) cells/ml. Addition of AS to the bacterial culture prior to inoculation did not alter transformation frequency.
Keywords
ARACHIS-HYPOGAEA, PEANUT, TRANSFORMATION, AGROBACTERIUM-TUMEFACIENS, RESISTANT ALFALFA, GENE-TRANSFER, PLANTS, DNA, REGENERATION, SPECIFICITY, GENOTYPE, STRAIN

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Citation

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Chicago
Mansur, Elisabeth A, Cristiano Lacorte, Valéria G de Freitas, Dulce De Oliveira, Benedikt Timmerman, and Antônio R Cordeiro. 1993. “Regulation of Transformation Efficiency of Peanut (Arachis Hypogaea L.) Explants by Agrobacterium Tumefaciens.” Plant Science 89 (1): 93–99.
APA
Mansur, E. A., Lacorte, C., de Freitas, V. G., De Oliveira, D., Timmerman, B., & Cordeiro, A. R. (1993). Regulation of transformation efficiency of peanut (Arachis hypogaea L.) explants by Agrobacterium tumefaciens. PLANT SCIENCE, 89(1), 93–99.
Vancouver
1.
Mansur EA, Lacorte C, de Freitas VG, De Oliveira D, Timmerman B, Cordeiro AR. Regulation of transformation efficiency of peanut (Arachis hypogaea L.) explants by Agrobacterium tumefaciens. PLANT SCIENCE. 1993;89(1):93–9.
MLA
Mansur, Elisabeth A, Cristiano Lacorte, Valéria G de Freitas, et al. “Regulation of Transformation Efficiency of Peanut (Arachis Hypogaea L.) Explants by Agrobacterium Tumefaciens.” PLANT SCIENCE 89.1 (1993): 93–99. Print.
@article{2110358,
  abstract     = {Some of the factors that modulate the transformation efficiency of regenerable tissues of peanut (Arachis hypogaea L.) were examined, using a tumor induction system based on strain A281 harbouring a binary vector. Factors examined were the composition of media, the cocultivation period, tissue type, bacterial inoculum concentration and pretreatment with acetosyringone (AS). Transformation efficiency was greatest when leaf or cotyledon explants were cocultivated on solid rather than liquid medium for 48 h. Highest transformation rates were observed on leaves from 7-10-day-old seedlings, with inoculum densities of 10(9)-5 x 10(9) cells/ml. Addition of AS to the bacterial culture prior to inoculation did not alter transformation frequency.},
  author       = {Mansur, Elisabeth A and Lacorte, Cristiano and de Freitas, Val{\'e}ria G and De Oliveira, Dulce and Timmerman, Benedikt and Cordeiro, Ant{\^o}nio R},
  issn         = {0168-9452},
  journal      = {PLANT SCIENCE},
  keyword      = {ARACHIS-HYPOGAEA,PEANUT,TRANSFORMATION,AGROBACTERIUM-TUMEFACIENS,RESISTANT ALFALFA,GENE-TRANSFER,PLANTS,DNA,REGENERATION,SPECIFICITY,GENOTYPE,STRAIN},
  language     = {eng},
  number       = {1},
  pages        = {93--99},
  title        = {Regulation of transformation efficiency of peanut (Arachis hypogaea L.) explants by Agrobacterium tumefaciens},
  url          = {http://dx.doi.org/10.1016/0168-9452(93)90174-X},
  volume       = {89},
  year         = {1993},
}

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