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Electroporation of intact embryonic leaflets of peanut: gene transfer and stimulation of regeneration capacity

VLM de Pádua, MC Pestana, M Margis-Pinheiro, Dulce De Oliveira UGent and E Mansur (2000) IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT. 36(5). p.374-378
abstract
Direct gene transfer into peanut intact embryonic leaflets was performed through electroporation. In transient beta -glucuronidase expression assays, maximal expression was obtained by using pulses of 625 V cm(-1) in EPRm (modified electroporation) buffer supplemented with 75 muM NaCl. Kanamycin-resistant plants were obtained, and the presence of the nptII gene was demonstrated by PCR analysis. The positive effect electroporation on the efficiency of in vitro regeneration was demonstrated. Explants submitted to field strengths between 500 and 625 V cm(-1) displayed a significantly increased number of shoots and originated faster growing calluses relative to control explants. Whereas in control explants callus formation occurred only at the petiolule, electroporated leaflets developed additional organogenic calluses on the foliar lamina.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
intact tissue, Arachis hypogaea, organogenesis, transformation, transient expression, TRANSIENT EXPRESSION, TRANSGENIC MAIZE, FOREIGN GENES, PLANTS, CULTURE, TISSUE, CELLS, TRANSFORMATION, PROTOPLASTS, DNA
journal title
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
In Vitro Cell. Dev. Biol.-Plant
volume
36
issue
5
pages
374 - 378
Web of Science type
Article
Web of Science id
000166709400013
ISSN
1054-5476
DOI
10.1007/s11627-000-0067-6
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2110269
handle
http://hdl.handle.net/1854/LU-2110269
date created
2012-05-22 11:53:14
date last changed
2016-12-19 15:42:56
@article{2110269,
  abstract     = {Direct gene transfer into peanut intact embryonic leaflets was performed through electroporation. In transient beta -glucuronidase expression assays, maximal expression was obtained by using pulses of 625 V cm(-1) in EPRm (modified electroporation) buffer supplemented with 75 muM NaCl. Kanamycin-resistant plants were obtained, and the presence of the nptII gene was demonstrated by PCR analysis. The positive effect electroporation on the efficiency of in vitro regeneration was demonstrated. Explants submitted to field strengths between 500 and 625 V cm(-1) displayed a significantly increased number of shoots and originated faster growing calluses relative to control explants. Whereas in control explants callus formation occurred only at the petiolule, electroporated leaflets developed additional organogenic calluses on the foliar lamina.},
  author       = {de P{\'a}dua, VLM and Pestana, MC and Margis-Pinheiro, M and De Oliveira, Dulce and Mansur, E},
  issn         = {1054-5476},
  journal      = {IN VITRO CELLULAR \& DEVELOPMENTAL BIOLOGY-PLANT},
  keyword      = {intact tissue,Arachis hypogaea,organogenesis,transformation,transient expression,TRANSIENT EXPRESSION,TRANSGENIC MAIZE,FOREIGN GENES,PLANTS,CULTURE,TISSUE,CELLS,TRANSFORMATION,PROTOPLASTS,DNA},
  language     = {eng},
  number       = {5},
  pages        = {374--378},
  title        = {Electroporation of intact embryonic leaflets of peanut: gene transfer and stimulation of regeneration capacity},
  url          = {http://dx.doi.org/10.1007/s11627-000-0067-6},
  volume       = {36},
  year         = {2000},
}

Chicago
de Pádua, VLM, MC Pestana, M Margis-Pinheiro, Dulce De Oliveira, and E Mansur. 2000. “Electroporation of Intact Embryonic Leaflets of Peanut: Gene Transfer and Stimulation of Regeneration Capacity.” In Vitro Cellular & Developmental Biology-plant 36 (5): 374–378.
APA
de Pádua, V., Pestana, M., Margis-Pinheiro, M., De Oliveira, D., & Mansur, E. (2000). Electroporation of intact embryonic leaflets of peanut: gene transfer and stimulation of regeneration capacity. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 36(5), 374–378.
Vancouver
1.
de Pádua V, Pestana M, Margis-Pinheiro M, De Oliveira D, Mansur E. Electroporation of intact embryonic leaflets of peanut: gene transfer and stimulation of regeneration capacity. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT. 2000;36(5):374–8.
MLA
de Pádua, VLM, MC Pestana, M Margis-Pinheiro, et al. “Electroporation of Intact Embryonic Leaflets of Peanut: Gene Transfer and Stimulation of Regeneration Capacity.” IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 36.5 (2000): 374–378. Print.