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Quantitative proteome profiling of C. burnetii under tetracycline stress conditions

Iosif Vranakis, Pieter-Jan De Bock, Anastasia Papadioti, Yannis Tselentis, Kris Gevaert UGent, Georgios Tsiotis and Anna Psaroulaki (2012) PLOS ONE. 7(3).
abstract
The recommended antibiotic regimen against Coxiella burnetii, the etiological agent of Q fever, is based on a semi-synthetic, second-generation tetracycline, doxycycline. Here, we report on the comparison of the proteomes of a C. burnetii reference strain either cultured under control conditions or under tetracycline stress conditions. Using the MS-driven combined fractional diagonal chromatography proteomics technique, out of the 531 proteins identified, 5 and 19 proteins were found significantly up-and down-regulated respectively, under tetracycline stress. Although the predicted cellular functions of these regulated proteins did not point to known tetracycline resistance mechanisms, our data clearly reveal the plasticity of the proteome of C. burnetii to battle tetracycline stress. Finally, we raise several plausible hypotheses that could further lead to more focused experiments on studying tetracycline resistance in C. burnetii and thus reduced treatment failures of Q fever.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SUBUNIT INTERFACE, SIGNAL PEPTIDASES, RIBOSOMAL PROTECTION, PSEUDOMONAS-AERUGINOSA, LEADER PEPTIDASE, Q-FEVER, ESCHERICHIA-COLI, ANTIBIOTIC-RESISTANCE GENES, DRUG-RESISTANCE, BACTERIUM COXIELLA-BURNETII
journal title
PLOS ONE
PLoS One
volume
7
issue
3
article number
e33599
pages
8 pages
Web of Science type
Article
Web of Science id
000303309100067
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.73 (2012)
JCR rank
7/56 (2012)
JCR quartile
1 (2012)
ISSN
1932-6203
DOI
10.1371/journal.pone.0033599
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2110093
handle
http://hdl.handle.net/1854/LU-2110093
date created
2012-05-22 10:20:59
date last changed
2016-12-21 15:41:58
@article{2110093,
  abstract     = {The recommended antibiotic regimen against Coxiella burnetii, the etiological agent of Q fever, is based on a semi-synthetic, second-generation tetracycline, doxycycline. Here, we report on the comparison of the proteomes of a C. burnetii reference strain either cultured under control conditions or under tetracycline stress conditions. Using the MS-driven combined fractional diagonal chromatography proteomics technique, out of the 531 proteins identified, 5 and 19 proteins were found significantly up-and down-regulated respectively, under tetracycline stress. Although the predicted cellular functions of these regulated proteins did not point to known tetracycline resistance mechanisms, our data clearly reveal the plasticity of the proteome of C. burnetii to battle tetracycline stress. Finally, we raise several plausible hypotheses that could further lead to more focused experiments on studying tetracycline resistance in C. burnetii and thus reduced treatment failures of Q fever.},
  articleno    = {e33599},
  author       = {Vranakis, Iosif and De Bock, Pieter-Jan and Papadioti, Anastasia and Tselentis, Yannis and Gevaert, Kris and Tsiotis, Georgios and Psaroulaki, Anna},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {SUBUNIT INTERFACE,SIGNAL PEPTIDASES,RIBOSOMAL PROTECTION,PSEUDOMONAS-AERUGINOSA,LEADER PEPTIDASE,Q-FEVER,ESCHERICHIA-COLI,ANTIBIOTIC-RESISTANCE GENES,DRUG-RESISTANCE,BACTERIUM COXIELLA-BURNETII},
  language     = {eng},
  number       = {3},
  pages        = {8},
  title        = {Quantitative proteome profiling of C. burnetii under tetracycline stress conditions},
  url          = {http://dx.doi.org/10.1371/journal.pone.0033599},
  volume       = {7},
  year         = {2012},
}

Chicago
Vranakis, Iosif, Pieter-Jan De Bock, Anastasia Papadioti, Yannis Tselentis, Kris Gevaert, Georgios Tsiotis, and Anna Psaroulaki. 2012. “Quantitative Proteome Profiling of C. Burnetii Under Tetracycline Stress Conditions.” Plos One 7 (3).
APA
Vranakis, I., De Bock, P.-J., Papadioti, A., Tselentis, Y., Gevaert, K., Tsiotis, G., & Psaroulaki, A. (2012). Quantitative proteome profiling of C. burnetii under tetracycline stress conditions. PLOS ONE, 7(3).
Vancouver
1.
Vranakis I, De Bock P-J, Papadioti A, Tselentis Y, Gevaert K, Tsiotis G, et al. Quantitative proteome profiling of C. burnetii under tetracycline stress conditions. PLOS ONE. 2012;7(3).
MLA
Vranakis, Iosif, Pieter-Jan De Bock, Anastasia Papadioti, et al. “Quantitative Proteome Profiling of C. Burnetii Under Tetracycline Stress Conditions.” PLOS ONE 7.3 (2012): n. pag. Print.