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Salmonella Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages

Alexander Van Parys UGent, Filip Boyen UGent, Elin Verbrugghe UGent, Bregje Leyman UGent, Bram Flahou UGent, Freddy Haesebrouck UGent and Frank Pasmans UGent (2012) VETERINARY RESEARCH. 43.
abstract
Foodborne salmonellosis is one of the most important bacterial zoonotic diseases worldwide. Salmonella Typhimurium is the serovar most frequently isolated from persistently infected slaughter pigs in Europe. Circumvention of the host’s immune system by Salmonella might contribute to persistent infection of pigs. In the present study, we found that Salmonella Typhimurium strain 112910a specifically downregulated MHC II, but not MHC I, expression on porcine macrophages in a Salmonella pathogenicity island (SPI)-1 and SPI-2 dependent way. Salmonella induced downregulation of MHC II expression and intracellular proliferation of Salmonella in macrophages were significantly impaired after opsonization with Salmonella specific antibodies prior to inoculation. Furthermore, the capacity to downregulate MHC II expression on macrophages differed significantly among Salmonella strains, independently of strain specific differences in invasion capacity, Salmonella induced cytotoxicity and altered macrophage activation status. The fact that strain specific differences in MHC II downregulation did not correlate with the extent of in vitro SPI-1 or SPI-2 gene expression indicates that other factors are involved in MHC II downregulation as well. Since Salmonella strain dependent interference with the pig’s immune response through downregulation of MHC II expression might indicate that certain Salmonella strains are more likely to escape serological detection, our findings are of major interest for Salmonella monitoring programs primarily based on serology.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IN-VITRO, ENTERICA SEROVAR TYPHIMURIUM, TIME-COURSE, PIGS, INFECTION, SUPPRESSION, GENES, IDENTIFICATION, COLONIZATION, EPIDEMIOLOGY
journal title
VETERINARY RESEARCH
Vet. Res.
volume
43
article_number
52
pages
14 pages
Web of Science type
Article
Web of Science id
000306646500001
ISSN
1297-9716
DOI
10.1186/1297-9716-43-52
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2109235
handle
http://hdl.handle.net/1854/LU-2109235
date created
2012-05-19 01:11:33
date last changed
2012-09-11 14:18:26
@article{2109235,
  abstract     = {Foodborne salmonellosis is one of the most important bacterial zoonotic diseases worldwide. Salmonella Typhimurium is the serovar most frequently isolated from persistently infected slaughter pigs in Europe. Circumvention of the host{\textquoteright}s immune system by Salmonella might contribute to persistent infection of pigs. In the present study, we found that Salmonella Typhimurium strain 112910a specifically downregulated MHC II, but not MHC I, expression on porcine macrophages in a Salmonella pathogenicity island (SPI)-1 and SPI-2 dependent way. Salmonella induced downregulation of MHC II expression and intracellular proliferation of Salmonella in macrophages were significantly impaired after opsonization with Salmonella specific antibodies prior to inoculation. Furthermore, the capacity to downregulate MHC II expression on macrophages differed significantly among Salmonella strains, independently of strain specific differences in invasion capacity, Salmonella induced cytotoxicity and altered macrophage activation status. The fact that strain specific differences in MHC II downregulation did not correlate with the extent of in vitro SPI-1 or SPI-2 gene expression indicates that other factors are involved in MHC II downregulation as well. Since Salmonella strain dependent interference with the pig{\textquoteright}s immune response through downregulation of MHC II expression might indicate that certain Salmonella strains are more likely to escape serological detection, our findings are of major interest for Salmonella monitoring programs primarily based on serology.},
  articleno    = {52},
  author       = {Van Parys, Alexander and Boyen, Filip and Verbrugghe, Elin and Leyman, Bregje and Flahou, Bram and Haesebrouck, Freddy and Pasmans, Frank},
  issn         = {1297-9716},
  journal      = {VETERINARY RESEARCH},
  keyword      = {IN-VITRO,ENTERICA SEROVAR TYPHIMURIUM,TIME-COURSE,PIGS,INFECTION,SUPPRESSION,GENES,IDENTIFICATION,COLONIZATION,EPIDEMIOLOGY},
  language     = {eng},
  pages        = {14},
  title        = {Salmonella Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages},
  url          = {http://dx.doi.org/10.1186/1297-9716-43-52},
  volume       = {43},
  year         = {2012},
}

Chicago
Van Parys, Alexander, Filip Boyen, Elin Verbrugghe, Bregje Leyman, Bram Flahou, Freddy Haesebrouck, and Frank Pasmans. 2012. “Salmonella Typhimurium Induces SPI-1 and SPI-2 Regulated and Strain Dependent Downregulation of MHC II Expression on Porcine Alveolar Macrophages.” Veterinary Research 43.
APA
Van Parys, A., Boyen, F., Verbrugghe, E., Leyman, B., Flahou, B., Haesebrouck, F., & Pasmans, F. (2012). Salmonella Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages. VETERINARY RESEARCH, 43.
Vancouver
1.
Van Parys A, Boyen F, Verbrugghe E, Leyman B, Flahou B, Haesebrouck F, et al. Salmonella Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages. VETERINARY RESEARCH. 2012;43.
MLA
Van Parys, Alexander, Filip Boyen, Elin Verbrugghe, et al. “Salmonella Typhimurium Induces SPI-1 and SPI-2 Regulated and Strain Dependent Downregulation of MHC II Expression on Porcine Alveolar Macrophages.” VETERINARY RESEARCH 43 (2012): n. pag. Print.