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Sulfide- and nitrite-dependent nitric oxide production in the intestinal tract

Joan Vermeiren, Tom Van de Wiele UGent, Glynn Van Nieuwenhuyse, Pascal Boeckx UGent, Willy Verstraete and Nico Boon UGent (2012) MICROBIAL BIOTECHNOLOGY. 5(3). p.379-387
abstract
In the gut ecosystem, nitric oxide (NO) has been described to have damaging effects on the energy metabolism of colonocytes. Described mechanisms of NO production are microbial reduction of nitrate via nitrite to NO and conversion of l-arginine by NO synthase. The aim of this study was to investigate whether dietary compounds can stimulate the production of NO by representative cultures of the human intestinal microbiota and whether this correlates to other processes in the intestinal tract. We have found that the addition of a reduced sulfur compound, i.e. cysteine, contributed to NO formation. This increase was ascribed to higher sulfide concentrations generated from cysteine that in turn promoted the chemical conversion of nitrite to NO. The NO release from nitrite was of the order of 4 parts per thousand at most. Overall, it was shown that two independent biological processes contribute to the chemical formation of NO in the intestinal tract: (i) the production of sulfide by fermentation of sulfur containing amino acids or reduction of sulfate by sulfate reducing bacteria, and (ii) the reduction of nitrate to nitrite. Our results indicate that dietary thiol compounds in combination with nitrate may contribute to colonocytes damaging processes by promoting NO formation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DESULFOVIBRIO-DESULFURICANS, BACTERIA, ULCERATIVE-COLITIS, MICROBIAL ECOSYSTEM, METABOLISM, HUMANS, SIMULATOR, NITRATE REDUCTASE, SYNTHASE ACTIVITY, CROHNS-DISEASE
journal title
MICROBIAL BIOTECHNOLOGY
Microb. Biotechnol.
editor
Nico Boon UGent and Willy Verstraete
volume
5
issue
3
issue title
Microbial resource management
pages
379 - 387
Web of Science type
Article
Web of Science id
000302858900007
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.214 (2012)
JCR rank
41/157 (2012)
JCR quartile
2 (2012)
ISSN
1751-7907
DOI
10.1111/j.1751-7915.2011.00320.x
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2108831
handle
http://hdl.handle.net/1854/LU-2108831
date created
2012-05-16 14:25:47
date last changed
2017-07-28 13:08:14
@article{2108831,
  abstract     = {In the gut ecosystem, nitric oxide (NO) has been described to have damaging effects on the energy metabolism of colonocytes. Described mechanisms of NO production are microbial reduction of nitrate via nitrite to NO and conversion of l-arginine by NO synthase. The aim of this study was to investigate whether dietary compounds can stimulate the production of NO by representative cultures of the human intestinal microbiota and whether this correlates to other processes in the intestinal tract. We have found that the addition of a reduced sulfur compound, i.e. cysteine, contributed to NO formation. This increase was ascribed to higher sulfide concentrations generated from cysteine that in turn promoted the chemical conversion of nitrite to NO. The NO release from nitrite was of the order of 4 parts per thousand at most. Overall, it was shown that two independent biological processes contribute to the chemical formation of NO in the intestinal tract: (i) the production of sulfide by fermentation of sulfur containing amino acids or reduction of sulfate by sulfate reducing bacteria, and (ii) the reduction of nitrate to nitrite. Our results indicate that dietary thiol compounds in combination with nitrate may contribute to colonocytes damaging processes by promoting NO formation.},
  author       = {Vermeiren, Joan and Van de Wiele, Tom and Van Nieuwenhuyse, Glynn and Boeckx, Pascal and Verstraete, Willy and Boon, Nico},
  editor       = {Boon, Nico and Verstraete, Willy},
  issn         = {1751-7907},
  journal      = {MICROBIAL BIOTECHNOLOGY},
  keyword      = {DESULFOVIBRIO-DESULFURICANS,BACTERIA,ULCERATIVE-COLITIS,MICROBIAL ECOSYSTEM,METABOLISM,HUMANS,SIMULATOR,NITRATE REDUCTASE,SYNTHASE ACTIVITY,CROHNS-DISEASE},
  language     = {eng},
  number       = {3},
  pages        = {379--387},
  title        = {Sulfide- and nitrite-dependent nitric oxide production in the intestinal tract},
  url          = {http://dx.doi.org/10.1111/j.1751-7915.2011.00320.x},
  volume       = {5},
  year         = {2012},
}

Chicago
Vermeiren, Joan, Tom Van de Wiele, Glynn Van Nieuwenhuyse, Pascal Boeckx, Willy Verstraete, and Nico Boon. 2012. “Sulfide- and Nitrite-dependent Nitric Oxide Production in the Intestinal Tract.” Ed. Nico Boon and Willy Verstraete. Microbial Biotechnology 5 (3): 379–387.
APA
Vermeiren, J., Van de Wiele, T., Van Nieuwenhuyse, G., Boeckx, P., Verstraete, W., & Boon, N. (2012). Sulfide- and nitrite-dependent nitric oxide production in the intestinal tract. (N. Boon & W. Verstraete, Eds.)MICROBIAL BIOTECHNOLOGY, 5(3), 379–387.
Vancouver
1.
Vermeiren J, Van de Wiele T, Van Nieuwenhuyse G, Boeckx P, Verstraete W, Boon N. Sulfide- and nitrite-dependent nitric oxide production in the intestinal tract. Boon N, Verstraete W, editors. MICROBIAL BIOTECHNOLOGY. 2012;5(3):379–87.
MLA
Vermeiren, Joan, Tom Van de Wiele, Glynn Van Nieuwenhuyse, et al. “Sulfide- and Nitrite-dependent Nitric Oxide Production in the Intestinal Tract.” Ed. Nico Boon & Willy Verstraete. MICROBIAL BIOTECHNOLOGY 5.3 (2012): 379–387. Print.