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Miniaturized extinction culturing is the preferred strategy for rapid isolation of fast-growing methane-oxidizing bacteria

Sven Hoefman UGent, David van der Ha UGent, Paul De Vos UGent, Nico Boon UGent and Kim Heylen UGent (2012) MICROBIAL BIOTECHNOLOGY. 5(3). p.368-378
abstract
Methane-oxidizing bacteria (MOB) have a large potential as a microbial sink for the greenhouse gas methane as well as for biotechnological purposes. However, their application in biotechnology has so far been hampered, in part due to the relative slow growth rate of the available strains. To enable the availability of novel strains, this study compares the isolation of MOB by conventional dilution plating with miniaturized extinction culturing, both performed after an initial enrichment step. The extinction approach rendered 22 MOB isolates from four environmental samples, while no MOB could be isolated by plating. In most cases, extinction culturing immediately yielded MOB monocultures making laborious purification redundant. Both type I (Methylomonas spp.) and type II (Methylosinus sp.) MOB were isolated. The isolated methanotrophic diversity represented at least 11 different strains and several novel species based on 16S rRNA gene sequence dissimilarity. These strains possessed the particulate (100%) and soluble (64%) methane monooxygenase gene. Also, 73% of the strains could be linked to a highly active fast-growing mixed MOB community. In conclusion, miniaturized extinction culturing was more efficient in rapidly isolating numerous MOB requiring little effort and fewer materials, compared with the more widely applied plating procedure. This miniaturized approach allowed straightforward isolation and could be very useful for subsequent screening of desired characteristics, in view of their future biotechnological potential.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
16S RIBOSOMAL-RNA, SP NOV., GEN. NOV., MARINE-BACTERIA, MOLECULAR CHARACTERIZATION, IMPROVED CULTURABILITY, ACIDOPHILIC BACTERIUM, SAR11 STRAINS, DILUTION CULTURE, METHANOTROPHIC BACTERIA
journal title
MICROBIAL BIOTECHNOLOGY
Microb. Biotechnol.
editor
Nico Boon UGent and Willy Verstraete UGent
volume
5
issue
3
issue title
Microbial resource management
pages
368 - 378
Web of Science type
Article
Web of Science id
000302858900006
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.214 (2012)
JCR rank
41/157 (2012)
JCR quartile
2 (2012)
ISSN
1751-7907
DOI
10.1111/j.1751-7915.2011.00314.x
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2108791
handle
http://hdl.handle.net/1854/LU-2108791
date created
2012-05-16 13:59:56
date last changed
2014-05-26 10:02:59
@article{2108791,
  abstract     = {Methane-oxidizing bacteria (MOB) have a large potential as a microbial sink for the greenhouse gas methane as well as for biotechnological purposes. However, their application in biotechnology has so far been hampered, in part due to the relative slow growth rate of the available strains. To enable the availability of novel strains, this study compares the isolation of MOB by conventional dilution plating with miniaturized extinction culturing, both performed after an initial enrichment step. The extinction approach rendered 22 MOB isolates from four environmental samples, while no MOB could be isolated by plating. In most cases, extinction culturing immediately yielded MOB monocultures making laborious purification redundant. Both type I (Methylomonas spp.) and type II (Methylosinus sp.) MOB were isolated. The isolated methanotrophic diversity represented at least 11 different strains and several novel species based on 16S rRNA gene sequence dissimilarity. These strains possessed the particulate (100\%) and soluble (64\%) methane monooxygenase gene. Also, 73\% of the strains could be linked to a highly active fast-growing mixed MOB community. In conclusion, miniaturized extinction culturing was more efficient in rapidly isolating numerous MOB requiring little effort and fewer materials, compared with the more widely applied plating procedure. This miniaturized approach allowed straightforward isolation and could be very useful for subsequent screening of desired characteristics, in view of their future biotechnological potential.},
  author       = {Hoefman, Sven and van der Ha, David and De Vos, Paul and Boon, Nico and Heylen, Kim},
  editor       = {Boon, Nico and Verstraete, Willy},
  issn         = {1751-7907},
  journal      = {MICROBIAL BIOTECHNOLOGY},
  keyword      = {16S RIBOSOMAL-RNA,SP NOV.,GEN. NOV.,MARINE-BACTERIA,MOLECULAR CHARACTERIZATION,IMPROVED CULTURABILITY,ACIDOPHILIC BACTERIUM,SAR11 STRAINS,DILUTION CULTURE,METHANOTROPHIC BACTERIA},
  language     = {eng},
  number       = {3},
  pages        = {368--378},
  title        = {Miniaturized extinction culturing is the preferred strategy for rapid isolation of fast-growing methane-oxidizing bacteria},
  url          = {http://dx.doi.org/10.1111/j.1751-7915.2011.00314.x},
  volume       = {5},
  year         = {2012},
}

Chicago
Hoefman, Sven, David van der Ha, Paul De Vos, Nico Boon, and Kim Heylen. 2012. “Miniaturized Extinction Culturing Is the Preferred Strategy for Rapid Isolation of Fast-growing Methane-oxidizing Bacteria.” Ed. Nico Boon and Willy Verstraete. Microbial Biotechnology 5 (3): 368–378.
APA
Hoefman, S., van der Ha, D., De Vos, P., Boon, N., & Heylen, K. (2012). Miniaturized extinction culturing is the preferred strategy for rapid isolation of fast-growing methane-oxidizing bacteria. (N. Boon & W. Verstraete, Eds.)MICROBIAL BIOTECHNOLOGY, 5(3), 368–378.
Vancouver
1.
Hoefman S, van der Ha D, De Vos P, Boon N, Heylen K. Miniaturized extinction culturing is the preferred strategy for rapid isolation of fast-growing methane-oxidizing bacteria. Boon N, Verstraete W, editors. MICROBIAL BIOTECHNOLOGY. 2012;5(3):368–78.
MLA
Hoefman, Sven, David van der Ha, Paul De Vos, et al. “Miniaturized Extinction Culturing Is the Preferred Strategy for Rapid Isolation of Fast-growing Methane-oxidizing Bacteria.” Ed. Nico Boon & Willy Verstraete. MICROBIAL BIOTECHNOLOGY 5.3 (2012): 368–378. Print.