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The plant defensin RsAFP2 induces cell wall stress, septin mislocalization and accumulation of ceramides in Candida albicans

Karin Thevissen, Patricia de Mello Tavares, Deming Xu, Jill Blankenship, Davy Vandenbosch UGent, Jolanta Idkowiak-Baldys, Gilmer Govaert, Anna Bink, Sonia Rozental and Piet WJ de Groot, et al. (2012) MOLECULAR MICROBIOLOGY. 84(1). p.166-180
abstract
The antifungal plant defensin RsAFP2 isolated from radish interacts with fungal glucosylceramides and induces apoptosis in Candida albicans. To further unravel the mechanism of RsAFP2 antifungal action and tolerance mechanisms, we screened a library of 2868 heterozygous C. albicans deletion mutants and identified 30 RsAFP2-hypersensitive mutants. The most prominent group of RsAFP2 tolerance genes was involved in cell wall integrity and hyphal growth/septin ring formation. Consistent with these genetic data, we demonstrated that RsAFP2 interacts with the cell wall of C. albicans, which also contains glucosylceramides, and activates the cell wall integrity pathway. Moreover, we found that RsAFP2 induces mislocalization of septins and blocks the yeast-to-hypha transition in C. albicans. Increased ceramide levels have previously been shown to result in apoptosis and septin mislocalization. Therefore, ceramide levels in C. albicans membranes were analysed following RsAFP2 treatment and, as expected, increased accumulation of phytoC24-ceramides in membranes of RsAFP2-treated C. albicans cells was detected. This is the first report on the interaction of a plant defensin with glucosylceramides in the fungal cell wall, causing cell wall stress, and on the effects of a defensin on septin localization and ceramide accumulation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
FUSARIUM-GRAMINEARUM, RAPHANUS-SATIVUS, NEUROSPORA-CRASSA, ANTIFUNGAL PLANT, USTILAGO-MAYDIS, FUNGAL GROWTH, LIPID-II, YEAST, BIOSYNTHESIS, PROTEINS
journal title
MOLECULAR MICROBIOLOGY
Mol. Microbiol.
volume
84
issue
1
pages
166 - 180
Web of Science type
Article
Web of Science id
000302013500013
JCR category
MICROBIOLOGY
JCR impact factor
4.961 (2012)
JCR rank
19/116 (2012)
JCR quartile
1 (2012)
ISSN
0950-382X
DOI
10.1111/j.1365-2958.2012.08017.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2108228
handle
http://hdl.handle.net/1854/LU-2108228
date created
2012-05-16 10:09:08
date last changed
2012-05-16 10:51:38
@article{2108228,
  abstract     = {The antifungal plant defensin RsAFP2 isolated from radish interacts with fungal glucosylceramides and induces apoptosis in Candida albicans. To further unravel the mechanism of RsAFP2 antifungal action and tolerance mechanisms, we screened a library of 2868 heterozygous C. albicans deletion mutants and identified 30 RsAFP2-hypersensitive mutants. The most prominent group of RsAFP2 tolerance genes was involved in cell wall integrity and hyphal growth/septin ring formation. Consistent with these genetic data, we demonstrated that RsAFP2 interacts with the cell wall of C. albicans, which also contains glucosylceramides, and activates the cell wall integrity pathway. Moreover, we found that RsAFP2 induces mislocalization of septins and blocks the yeast-to-hypha transition in C. albicans. Increased ceramide levels have previously been shown to result in apoptosis and septin mislocalization. Therefore, ceramide levels in C. albicans membranes were analysed following RsAFP2 treatment and, as expected, increased accumulation of phytoC24-ceramides in membranes of RsAFP2-treated C. albicans cells was detected. This is the first report on the interaction of a plant defensin with glucosylceramides in the fungal cell wall, causing cell wall stress, and on the effects of a defensin on septin localization and ceramide accumulation.},
  author       = {Thevissen, Karin and Tavares, Patricia de Mello and Xu, Deming and Blankenship, Jill and Vandenbosch, Davy and Idkowiak-Baldys, Jolanta and Govaert, Gilmer and Bink, Anna and Rozental, Sonia and de Groot, Piet WJ and Davis, Talya R and Kumamoto, Carol A and Vargas, Gabriele and Nimrichter, Leonardo and Coenye, Tom and Mitchell, Aaron and Roemer, Terry and Hannun, Yusuf A and Cammue, Bruno PA},
  issn         = {0950-382X},
  journal      = {MOLECULAR MICROBIOLOGY},
  keyword      = {FUSARIUM-GRAMINEARUM,RAPHANUS-SATIVUS,NEUROSPORA-CRASSA,ANTIFUNGAL PLANT,USTILAGO-MAYDIS,FUNGAL GROWTH,LIPID-II,YEAST,BIOSYNTHESIS,PROTEINS},
  language     = {eng},
  number       = {1},
  pages        = {166--180},
  title        = {The plant defensin RsAFP2 induces cell wall stress, septin mislocalization and accumulation of ceramides in Candida albicans},
  url          = {http://dx.doi.org/10.1111/j.1365-2958.2012.08017.x},
  volume       = {84},
  year         = {2012},
}

Chicago
Thevissen, Karin, Patricia de Mello Tavares, Deming Xu, Jill Blankenship, Davy Vandenbosch, Jolanta Idkowiak-Baldys, Gilmer Govaert, et al. 2012. “The Plant Defensin RsAFP2 Induces Cell Wall Stress, Septin Mislocalization and Accumulation of Ceramides in Candida Albicans.” Molecular Microbiology 84 (1): 166–180.
APA
Thevissen, K., Tavares, P. de M., Xu, D., Blankenship, J., Vandenbosch, D., Idkowiak-Baldys, J., Govaert, G., et al. (2012). The plant defensin RsAFP2 induces cell wall stress, septin mislocalization and accumulation of ceramides in Candida albicans. MOLECULAR MICROBIOLOGY, 84(1), 166–180.
Vancouver
1.
Thevissen K, Tavares P de M, Xu D, Blankenship J, Vandenbosch D, Idkowiak-Baldys J, et al. The plant defensin RsAFP2 induces cell wall stress, septin mislocalization and accumulation of ceramides in Candida albicans. MOLECULAR MICROBIOLOGY. 2012;84(1):166–80.
MLA
Thevissen, Karin, Patricia de Mello Tavares, Deming Xu, et al. “The Plant Defensin RsAFP2 Induces Cell Wall Stress, Septin Mislocalization and Accumulation of Ceramides in Candida Albicans.” MOLECULAR MICROBIOLOGY 84.1 (2012): 166–180. Print.