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Glycine and its N-methylated analogues cause pH-dependent membrane damage to enterotoxigenic Escherichia coli

Donna Vanhauteghem UGent, Geert Janssens UGent, A Lauwaerts, Stanislas Sys UGent, Filip Boyen UGent, Isabelle Kalmar UGent and Evelyne Meyer UGent (2012) AMINO ACIDS. 43(1). p.245-253
abstract
The current study first investigates the emulsifying potential of glycine and its N-methylated derivatives N-methylglycine (sarcosine), N,N-dimethylglycine (DMG) and N,N,N-trimethylglycine (betaine) under varying pH conditions. Subsequently, the effect of these test compounds on the membrane integrity of enterotoxigenic Escherichia coli (ETEC) was evaluated. Oil in water emulsions containing each compound show that DMG is a more potent enhancer of emulsification than glycine, sarcosine and betaine under the conditions tested. Flow cytometry was used to investigate whether the emulsifying potential is associated with an effect on ETEC membrane integrity. The bacteria were exposed to each of the test compounds under varying pH conditions and membrane integrity was assessed using the LIVE/DEAD BacLight kit. Results show a membrane deteriorating effect caused by glycine, sarcosine and DMG, but not by betaine. This effect is pH- and time-dependent and has an apparent threshold at pH 9.0. Conventional plate counts confirmed concomitant changes in culturability of the membrane comprised bacteria.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Emulsification, Alkaline stress, Membrane integrity, Flow cytometry, Glycine, Methylamine, CONDENSED MOLASSES SOLUBLES, NUTRIENT DIGESTIBILITY, N, N-DIMETHYLGLYCINE SUPPLEMENTATION, CHOLINE TRANSPORTER, OSMOTIC-STRESS, ALKALINE PH, BETAINE, BACTERIA, COMBINATION, SURFACTANTS
journal title
AMINO ACIDS
Amino Acids
volume
43
issue
1
issue title
Carnosine in exercise and disease
pages
245 - 253
Web of Science type
Article
Web of Science id
000305210800025
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
3.914 (2012)
JCR rank
86/288 (2012)
JCR quartile
2 (2012)
ISSN
0939-4451
DOI
10.1007/s00726-011-1068-y
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2108200
handle
http://hdl.handle.net/1854/LU-2108200
date created
2012-05-16 09:59:31
date last changed
2015-06-17 10:02:41
@article{2108200,
  abstract     = {The current study first investigates the emulsifying potential of glycine and its N-methylated derivatives N-methylglycine (sarcosine), N,N-dimethylglycine (DMG) and N,N,N-trimethylglycine (betaine) under varying pH conditions. Subsequently, the effect of these test compounds on the membrane integrity of enterotoxigenic Escherichia coli (ETEC) was evaluated. Oil in water emulsions containing each compound show that DMG is a more potent enhancer of emulsification than glycine, sarcosine and betaine under the conditions tested. Flow cytometry was used to investigate whether the emulsifying potential is associated with an effect on ETEC membrane integrity. The bacteria were exposed to each of the test compounds under varying pH conditions and membrane integrity was assessed using the LIVE/DEAD BacLight kit. Results show a membrane deteriorating effect caused by glycine, sarcosine and DMG, but not by betaine. This effect is pH- and time-dependent and has an apparent threshold at pH 9.0. Conventional plate counts confirmed concomitant changes in culturability of the membrane comprised bacteria.},
  author       = {Vanhauteghem, Donna and Janssens, Geert and Lauwaerts, A and Sys, Stanislas and Boyen, Filip and Kalmar, Isabelle and Meyer, Evelyne},
  issn         = {0939-4451},
  journal      = {AMINO ACIDS},
  keyword      = {Emulsification,Alkaline stress,Membrane integrity,Flow cytometry,Glycine,Methylamine,CONDENSED MOLASSES SOLUBLES,NUTRIENT DIGESTIBILITY,N,N-DIMETHYLGLYCINE SUPPLEMENTATION,CHOLINE TRANSPORTER,OSMOTIC-STRESS,ALKALINE PH,BETAINE,BACTERIA,COMBINATION,SURFACTANTS},
  language     = {eng},
  number       = {1},
  pages        = {245--253},
  title        = {Glycine and its N-methylated analogues cause pH-dependent membrane damage to enterotoxigenic Escherichia coli},
  url          = {http://dx.doi.org/10.1007/s00726-011-1068-y},
  volume       = {43},
  year         = {2012},
}

Chicago
Vanhauteghem, Donna, Geert Janssens, A Lauwaerts, Stanislas Sys, Filip Boyen, Isabelle Kalmar, and Evelyne Meyer. 2012. “Glycine and Its N-methylated Analogues Cause pH-dependent Membrane Damage to Enterotoxigenic Escherichia Coli.” Amino Acids 43 (1): 245–253.
APA
Vanhauteghem, D., Janssens, G., Lauwaerts, A., Sys, S., Boyen, F., Kalmar, I., & Meyer, E. (2012). Glycine and its N-methylated analogues cause pH-dependent membrane damage to enterotoxigenic Escherichia coli. AMINO ACIDS, 43(1), 245–253.
Vancouver
1.
Vanhauteghem D, Janssens G, Lauwaerts A, Sys S, Boyen F, Kalmar I, et al. Glycine and its N-methylated analogues cause pH-dependent membrane damage to enterotoxigenic Escherichia coli. AMINO ACIDS. 2012;43(1):245–53.
MLA
Vanhauteghem, Donna, Geert Janssens, A Lauwaerts, et al. “Glycine and Its N-methylated Analogues Cause pH-dependent Membrane Damage to Enterotoxigenic Escherichia Coli.” AMINO ACIDS 43.1 (2012): 245–253. Print.