Ghent University Academic Bibliography

Advanced

Dry heat stress stability of model peptide: buserelin

Matthias D'Hondt UGent and Bart De Spiegeleer UGent (2012) Scientific Afternoon, Posters.
abstract
Buserelin, an gonadotropin hormone releasing agonist peptide implemented in the treatment of prostate cancer, was subjected in its dry powder state to short-term heat exposure to evaluated its potential application in hot melt extrusion processing. Five different temperature and time conditions, ranging from 150°C to 180°C and 10 to 160 min, respectively, were evaluated (4 time points per temperature). Each experimental condition was performed in duplicate. A stability indicating UPLC-DAD (220 nm) method was used both for buserelin assay as degradant quantification. Linear regression, assuming first order degradation kinetics, was employed to calculate the Arrhenius activation energy (Ea) and frequency factor (A). During dry heat stressing, a change from solid to molten state was observed at 165°C. This change is also reflected in the Arrhenius regression curve, which is of sigmoidal nature. Given this change in state, two Arrhenius Ea were calculated, i.e. 217 and 87 kJ/mol for solid and molten state respectively. Three different degradant groups were observed using HPLC ESI/iontrap-MS: [M+H+] 453 amu, [M+H+] 902 amu and busereline diastereoisomers. The apparent degradation mechanisms included hydrolysis, dissociation of the tertiary butyl group on Ser6 and isomerization. Further structure elucidation is ongoing using nanoUPLC ESI/Orbitrap-MS.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
kinetic, dry heat stress, mass spectrometry, Peptides
in
Scientific Afternoon, Posters
publisher
Ghent University. Faculty of Pharmaceutical Sciences
place of publication
Ghent, Belgium
conference name
4th Scientific Afternoon
conference location
Ghent, Belgium
conference start
2012-05-16
conference end
2012-05-16
language
English
UGent publication?
yes
classification
C3
copyright statement
I have retained and own the full copyright for this publication
id
2106579
handle
http://hdl.handle.net/1854/LU-2106579
date created
2012-05-15 09:20:08
date last changed
2012-05-15 11:25:07
@inproceedings{2106579,
  abstract     = {Buserelin, an gonadotropin hormone releasing agonist peptide implemented in the treatment of prostate cancer, was subjected in its dry powder state to short-term heat exposure to evaluated its potential application in hot melt extrusion processing. Five different temperature and time conditions, ranging from 150{\textdegree}C to 180{\textdegree}C and 10 to 160 min, respectively, were evaluated (4 time points per temperature). Each experimental condition was performed in duplicate. A stability indicating UPLC-DAD (220 nm) method was used both for buserelin assay as degradant quantification. Linear regression, assuming first order degradation kinetics, was employed to calculate the Arrhenius activation energy (Ea) and frequency factor (A).
During dry heat stressing, a change from solid to molten state was observed at 165{\textdegree}C. This change is also reflected in the Arrhenius regression curve, which is of sigmoidal nature. Given this change in state, two Arrhenius Ea were calculated, i.e. 217 and 87 kJ/mol for solid and molten state respectively.
Three different degradant groups were observed using HPLC ESI/iontrap-MS: [M+H+] 453 amu, [M+H+] 902 amu and busereline diastereoisomers. The apparent degradation mechanisms included hydrolysis, dissociation of the tertiary butyl group on Ser6 and isomerization. Further structure elucidation is ongoing using nanoUPLC ESI/Orbitrap-MS.},
  author       = {D'Hondt, Matthias and De Spiegeleer, Bart},
  booktitle    = {Scientific Afternoon, Posters},
  keyword      = {kinetic,dry heat stress,mass spectrometry,Peptides},
  language     = {eng},
  location     = {Ghent, Belgium},
  publisher    = {Ghent University. Faculty of Pharmaceutical Sciences},
  title        = {Dry heat stress stability of model peptide: buserelin},
  year         = {2012},
}

Chicago
D’Hondt, Matthias, and Bart De Spiegeleer. 2012. “Dry Heat Stress Stability of Model Peptide: Buserelin.” In Scientific Afternoon, Posters. Ghent, Belgium: Ghent University. Faculty of Pharmaceutical Sciences.
APA
D’Hondt, Matthias, & De Spiegeleer, B. (2012). Dry heat stress stability of model peptide: buserelin. Scientific Afternoon, Posters. Presented at the 4th Scientific Afternoon, Ghent, Belgium: Ghent University. Faculty of Pharmaceutical Sciences.
Vancouver
1.
D’Hondt M, De Spiegeleer B. Dry heat stress stability of model peptide: buserelin. Scientific Afternoon, Posters. Ghent, Belgium: Ghent University. Faculty of Pharmaceutical Sciences; 2012.
MLA
D’Hondt, Matthias, and Bart De Spiegeleer. “Dry Heat Stress Stability of Model Peptide: Buserelin.” Scientific Afternoon, Posters. Ghent, Belgium: Ghent University. Faculty of Pharmaceutical Sciences, 2012. Print.