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Sugarcane genes differentially expressed in response to Puccinia melanocephala infection: identification and transcript profiling

María Ileana Oloriz Ortega UGent, Víctor Gil, Luis Rojas, Orelvis Portal, Yovanny Izquierdo, Elio Jiménez and Monica Höfte UGent (2012) PLANT CELL REPORTS. 31(5). p.955-969
abstract
Brown rust caused by the fungus Puccinia melanocephala is a major disease of sugarcane (Saccharum spp.). A sugarcane mutant, obtained by chemical mutagenesis of the susceptible variety B4362, showed a post-haustorial hypersensitive response (HR)-mediated resistance to the pathogen and was used to identify genes differentially expressed in response to P. melanocephala via suppression subtractive hybridization (SSH). Tester cDNA was derived from the brown rust-resistant mutant after inoculation with P. melanocephala, while driver cDNAs were obtained from the non-inoculated resistant mutant and the inoculated susceptible donor variety B4362. Database comparisons of the sequences of the SSH recombinant clones revealed that, of a subset of 89 non-redundant sequences, 88% had similarity to known functional genes, while 12% were of unknown function. Thirteen genes were selected for transcript profiling in the resistant mutant and the susceptible donor variety. Genes involved in glycolysis and C4 carbon fixation were up-regulated in both interactions probably due to disturbance of sugarcane carbon metabolism by the pathogen. Genes related with the nascent polypeptide associated complex, post-translational proteome modulation and autophagy were transcribed at higher levels in the compatible interaction. Up-regulation of a putative l-isoaspartyl O-methyltransferase S-adenosylmethionine gene in the compatible interaction may point to fungal manipulation of the cytoplasmatic methionine cycle. Genes coding for a putative no apical meristem protein, S-adenosylmethionine decarboxylase, non-specific lipid transfer protein, and GDP-l-galactose phosphorylase involved in ascorbic acid biosynthesis were up-regulated in the incompatible interaction at the onset of haustorium formation, and may contribute to the HR-mediated defense response in the rust-resistant mutant.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Sugarcane brown rust, Suppression subtractive hybridization, Hypersensitive response, POWDERY MILDEW, AMINO-ACIDS, RUST, ARABIDOPSIS, RESISTANCE, PROTEINS, WHEAT, POLYAMINES, DEFENSE, CELL
journal title
PLANT CELL REPORTS
Plant Cell Reports
volume
31
issue
5
pages
955 - 969
Web of Science type
Article
Web of Science id
000302813600015
JCR category
PLANT SCIENCES
JCR impact factor
2.509 (2012)
JCR rank
49/193 (2012)
JCR quartile
2 (2012)
ISSN
0721-7714
DOI
10.1007/s00299-011-1216-6
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2104531
handle
http://hdl.handle.net/1854/LU-2104531
date created
2012-05-10 17:32:34
date last changed
2012-05-11 09:29:11
@article{2104531,
  abstract     = {Brown rust caused by the fungus Puccinia melanocephala is a major disease of sugarcane (Saccharum spp.). A sugarcane mutant, obtained by chemical mutagenesis of the susceptible variety B4362, showed a post-haustorial hypersensitive response (HR)-mediated resistance to the pathogen and was used to identify genes differentially expressed in response to P. melanocephala via suppression subtractive hybridization (SSH). Tester cDNA was derived from the brown rust-resistant mutant after inoculation with P. melanocephala, while driver cDNAs were obtained from the non-inoculated resistant mutant and the inoculated susceptible donor variety B4362. Database comparisons of the sequences of the SSH recombinant clones revealed that, of a subset of 89 non-redundant sequences, 88\% had similarity to known functional genes, while 12\% were of unknown function. Thirteen genes were selected for transcript profiling in the resistant mutant and the susceptible donor variety. Genes involved in glycolysis and C4 carbon fixation were up-regulated in both interactions probably due to disturbance of sugarcane carbon metabolism by the pathogen. Genes related with the nascent polypeptide associated complex, post-translational proteome modulation and autophagy were transcribed at higher levels in the compatible interaction. Up-regulation of a putative l-isoaspartyl O-methyltransferase S-adenosylmethionine gene in the compatible interaction may point to fungal manipulation of the cytoplasmatic methionine cycle. Genes coding for a putative no apical meristem protein, S-adenosylmethionine decarboxylase, non-specific lipid transfer protein, and GDP-l-galactose phosphorylase involved in ascorbic acid biosynthesis were up-regulated in the incompatible interaction at the onset of haustorium formation, and may contribute to the HR-mediated defense response in the rust-resistant mutant.},
  author       = {Oloriz Ortega, Mar{\'i}a Ileana and Gil, V{\'i}ctor and Rojas, Luis and Portal, Orelvis and Izquierdo, Yovanny and Jim{\'e}nez, Elio and H{\"o}fte, Monica},
  issn         = {0721-7714},
  journal      = {PLANT CELL REPORTS},
  keyword      = {Sugarcane brown rust,Suppression subtractive hybridization,Hypersensitive response,POWDERY MILDEW,AMINO-ACIDS,RUST,ARABIDOPSIS,RESISTANCE,PROTEINS,WHEAT,POLYAMINES,DEFENSE,CELL},
  language     = {eng},
  number       = {5},
  pages        = {955--969},
  title        = {Sugarcane genes differentially expressed in response to Puccinia melanocephala infection: identification and transcript profiling},
  url          = {http://dx.doi.org/10.1007/s00299-011-1216-6},
  volume       = {31},
  year         = {2012},
}

Chicago
Oloriz Ortega, María Ileana, Víctor Gil, Luis Rojas, Orelvis Portal, Yovanny Izquierdo, Elio Jiménez, and Monica Höfte. 2012. “Sugarcane Genes Differentially Expressed in Response to Puccinia Melanocephala Infection: Identification and Transcript Profiling.” Plant Cell Reports 31 (5): 955–969.
APA
Oloriz Ortega, M. I., Gil, V., Rojas, L., Portal, O., Izquierdo, Y., Jiménez, E., & Höfte, M. (2012). Sugarcane genes differentially expressed in response to Puccinia melanocephala infection: identification and transcript profiling. PLANT CELL REPORTS, 31(5), 955–969.
Vancouver
1.
Oloriz Ortega MI, Gil V, Rojas L, Portal O, Izquierdo Y, Jiménez E, et al. Sugarcane genes differentially expressed in response to Puccinia melanocephala infection: identification and transcript profiling. PLANT CELL REPORTS. 2012;31(5):955–69.
MLA
Oloriz Ortega, María Ileana, Víctor Gil, Luis Rojas, et al. “Sugarcane Genes Differentially Expressed in Response to Puccinia Melanocephala Infection: Identification and Transcript Profiling.” PLANT CELL REPORTS 31.5 (2012): 955–969. Print.