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Induced heat shock protein production protects Artemia against vibriosis

Sri Kartik Baruah UGent (2012)
abstract
In this thesis, the effects of heat shock protein (Hsp) 70s and their inducers to control vibriosis were determined using Artemia franciscana as model organism. Results showed that feeding recombinant Artemia Hsp70 or E. coli DnaK protein (both has a high degree of structural conservation) protected Vibrio-challenged Artemia to a similar extent. Comparison of the peptide sequence of the two proteins showed a large degree of homology, especially in the peptide-binding domain (PBD) suggesting that the protective capacity of these Hsp70s might reside within this domain. Artemia fed with these Hsp70s and challenged with V. campbellii had enhanced prophenoloxidase (proPO) expression and activity levels, suggesting that Artemia were primed against Vibrio by Hsp70s. The assumption that the protective capacity of the Hsp70s resides within their PBD was also verified. Results showed that the C-terminal end (containing the PBD) of Artemia Hsp70 or DnaK is required for Hsp70-mediated protection of Vibrio-challenged Artemia. It was also observed that when Artemia were challenged with V. campbellii, prior feeding of the truncated Hsp70s significantly enhanced the proPO expression and activity levels, indicating that the epitope responsible for priming the proPO system, consequently boosting Artemia survival, might be located within this domain. In a further study, an extract from the prickly pear fruit Opuntia ficus indica named Pro-Tex® (containing the active molecule Tex-OE®), an Hsp inducer, was tested for its protective ability against abiotic and biotic stressors. Artemia pretreated with Tex-OE® (152 ppb) for 1 h accumulated endogenous Hsp70 and exhibited enhanced survival when subjected to thermal, hyperosmotic or Vibrio challenge. Tex-OE® pretreatment elicited the proPO at both mRNA and protein activity levels, and also induced nitric oxide level, indicating that Tex-OE® is undoubtedly beneficial for eliciting protective immunity in Artemia against V. campbellii and thus can potentially be a useful preventive treatment modality for vibrios.
Please use this url to cite or link to this publication:
author
promoter
UGent
organization
alternative title
Inductie van hitteschokproteïnen beschermt Artemia tegen vibriosis
year
type
dissertation (monograph)
subject
keyword
vibriosis, artemia, heat shock
pages
VIII, 209 pages
publisher
Ghent University. Faculty of Bioscience Engineering
place of publication
Ghent, Belgium
defense location
Gent : Faculteit Bio-ingenieurswetenschappen (A0.030)
defense date
2012-05-15 16:00
ISBN
9789059895225
language
English
UGent publication?
yes
classification
D1
additional info
dissertation consists of copyrighted material
copyright statement
I have transferred the copyright for this publication to the publisher
id
2100179
handle
http://hdl.handle.net/1854/LU-2100179
date created
2012-05-07 14:43:45
date last changed
2012-05-08 09:02:38
@phdthesis{2100179,
  abstract     = {In this thesis, the effects of heat shock protein (Hsp) 70s and their inducers to control vibriosis were determined using Artemia franciscana as model organism. Results showed that feeding recombinant Artemia Hsp70 or E. coli DnaK protein (both has a high degree of structural conservation) protected Vibrio-challenged Artemia to a similar extent. Comparison of the peptide sequence of the two proteins showed a large degree of homology, especially in the peptide-binding domain (PBD) suggesting that the protective capacity of these Hsp70s might reside within this domain. Artemia fed with these Hsp70s and challenged with V. campbellii had enhanced prophenoloxidase (proPO) expression and activity levels, suggesting that Artemia were primed against Vibrio by Hsp70s. The assumption that the protective capacity of the Hsp70s resides within their PBD was also verified. Results showed that the C-terminal end (containing the PBD) of Artemia Hsp70 or DnaK is required for Hsp70-mediated protection of Vibrio-challenged Artemia. It was also observed that when Artemia were challenged with V. campbellii, prior feeding of the truncated Hsp70s significantly enhanced the proPO expression and activity levels, indicating that the epitope responsible for priming the proPO system, consequently boosting Artemia survival, might be located within this domain.
In a further study, an extract from the prickly pear fruit Opuntia ficus indica named Pro-Tex{\textregistered} (containing the active molecule Tex-OE{\textregistered}), an Hsp inducer, was tested for its protective ability against abiotic and biotic stressors. Artemia pretreated with Tex-OE{\textregistered} (152 ppb) for 1 h accumulated endogenous Hsp70 and exhibited enhanced survival when subjected to thermal, hyperosmotic or Vibrio challenge. Tex-OE{\textregistered} pretreatment elicited the proPO at both mRNA and protein activity levels, and also induced nitric oxide level, indicating that Tex-OE{\textregistered} is undoubtedly beneficial for eliciting protective immunity in Artemia against V. campbellii and thus can potentially be a useful preventive treatment modality for vibrios.},
  author       = {Baruah, Sri Kartik},
  isbn         = {9789059895225},
  keyword      = {vibriosis,artemia,heat shock},
  language     = {eng},
  pages        = {VIII, 209},
  publisher    = {Ghent University. Faculty of Bioscience Engineering},
  school       = {Ghent University},
  title        = {Induced heat shock protein production protects Artemia against vibriosis},
  year         = {2012},
}

Chicago
Baruah, Sri Kartik. 2012. “Induced Heat Shock Protein Production Protects Artemia Against Vibriosis”. Ghent, Belgium: Ghent University. Faculty of Bioscience Engineering.
APA
Baruah, S. K. (2012). Induced heat shock protein production protects Artemia against vibriosis. Ghent University. Faculty of Bioscience Engineering, Ghent, Belgium.
Vancouver
1.
Baruah SK. Induced heat shock protein production protects Artemia against vibriosis. [Ghent, Belgium]: Ghent University. Faculty of Bioscience Engineering; 2012.
MLA
Baruah, Sri Kartik. “Induced Heat Shock Protein Production Protects Artemia Against Vibriosis.” 2012 : n. pag. Print.