Ghent University Academic Bibliography

Advanced

Proliferative response of the stem cell system during regeneration of the rostrum in Macrostomum lignano (Platyhelminthes)

Freija Verdoodt UGent, Wim Bert UGent, Marjolein Couvreur UGent, Katrien De Mulder UGent and Maxime Willems UGent (2012) CELL AND TISSUE RESEARCH. 347(2). p.397-406
abstract
Macrostomum lignano (Platyhelminthes) possesses pluripotent stem cells, also called neoblasts, which power its extraordinary regeneration capacity. We have examined the cellular dynamics of neoblasts during regeneration of the rostrum in M. lignano. First, using live squeeze observations, the growth curve of the rostrum was determined. Second, neoblasts were labelled with 5-bromo-2'-deoxyuridine (BrdU) and an anti-phospho-histone H3 mitosis marker (anti-phos-H3) to analyze their proliferative response to amputation. During the regeneration process, both S-and M-phase cells were present anterior to the eyes, a region that is devoid of proliferating cells during homeostasis. Furthermore, BrdU pulse experiments revealed a biphasic S-phase pattern, different from the pattern known to occur during regeneration of the tail plate in M. lignano. During a first systemic phase, S-phase numbers significantly increased, both in the region adjacent to the wound (the anterior segment) and the region far from the wound (the posterior segment). During the second, spatially restricted phase, S-phase numbers in the anterior segment rose to a peak at 3 to 5 days post-amputation (p-a), while in the posterior segment, S-phase activity approached control values again. A blastema, characterized as a build-up of S- and M-phase cells, was formed 1 day p-a. Altogether, our data present new insights into the cellular response of the neoblast system upon amputation, clearly demonstrating important differences from the situation known to occur during regeneration of the tail plate. Furthermore, the presence of proliferating cells in the region anterior to the eyes shows a clear alteration in stem cell regulation during regeneration.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Regeneration, PLANARIAN REGENERATION, S-phase, Platyhelminthes, Neoblasts, Macrostomum lignano, BLASTEMA FORMATION, PATTERN-FORMATION, DIFFERENTIATION, DYNAMICS, RHABDITOPHORA, MITOSIS, INTACT, MODEL
journal title
CELL AND TISSUE RESEARCH
Cell Tissue Res.
volume
347
issue
2
pages
397 - 406
Web of Science type
Article
Web of Science id
000302138300009
JCR category
CELL BIOLOGY
JCR impact factor
3.677 (2012)
JCR rank
78/181 (2012)
JCR quartile
2 (2012)
ISSN
0302-766X
DOI
10.1007/s00441-011-1299-y
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2097555
handle
http://hdl.handle.net/1854/LU-2097555
date created
2012-05-02 21:16:55
date last changed
2012-05-03 11:56:58
@article{2097555,
  abstract     = {Macrostomum lignano (Platyhelminthes) possesses pluripotent stem cells, also called neoblasts, which power its extraordinary regeneration capacity. We have examined the cellular dynamics of neoblasts during regeneration of the rostrum in M. lignano. First, using live squeeze observations, the growth curve of the rostrum was determined. Second, neoblasts were labelled with 5-bromo-2'-deoxyuridine (BrdU) and an anti-phospho-histone H3 mitosis marker (anti-phos-H3) to analyze their proliferative response to amputation. During the regeneration process, both S-and M-phase cells were present anterior to the eyes, a region that is devoid of proliferating cells during homeostasis. Furthermore, BrdU pulse experiments revealed a biphasic S-phase pattern, different from the pattern known to occur during regeneration of the tail plate in M. lignano. During a first systemic phase, S-phase numbers significantly increased, both in the region adjacent to the wound (the anterior segment) and the region far from the wound (the posterior segment). During the second, spatially restricted phase, S-phase numbers in the anterior segment rose to a peak at 3 to 5 days post-amputation (p-a), while in the posterior segment, S-phase activity approached control values again. A blastema, characterized as a build-up of S- and M-phase cells, was formed 1 day p-a. 
Altogether, our data present new insights into the cellular response of the neoblast system upon amputation, clearly demonstrating important differences from the situation known to occur during regeneration of the tail plate. Furthermore, the presence of proliferating cells in the region anterior to the eyes shows a clear alteration in stem cell regulation during regeneration.},
  author       = {Verdoodt, Freija and Bert, Wim and Couvreur, Marjolein and De Mulder, Katrien and Willems, Maxime},
  issn         = {0302-766X},
  journal      = {CELL AND TISSUE RESEARCH},
  keyword      = {Regeneration,PLANARIAN REGENERATION,S-phase,Platyhelminthes,Neoblasts,Macrostomum lignano,BLASTEMA FORMATION,PATTERN-FORMATION,DIFFERENTIATION,DYNAMICS,RHABDITOPHORA,MITOSIS,INTACT,MODEL},
  language     = {eng},
  number       = {2},
  pages        = {397--406},
  title        = {Proliferative response of the stem cell system during regeneration of the rostrum in Macrostomum lignano (Platyhelminthes)},
  url          = {http://dx.doi.org/10.1007/s00441-011-1299-y},
  volume       = {347},
  year         = {2012},
}

Chicago
Verdoodt, Freija, Wim Bert, Marjolein Couvreur, Katrien De Mulder, and Maxime Willems. 2012. “Proliferative Response of the Stem Cell System During Regeneration of the Rostrum in Macrostomum Lignano (Platyhelminthes).” Cell and Tissue Research 347 (2): 397–406.
APA
Verdoodt, F., Bert, W., Couvreur, M., De Mulder, K., & Willems, M. (2012). Proliferative response of the stem cell system during regeneration of the rostrum in Macrostomum lignano (Platyhelminthes). CELL AND TISSUE RESEARCH, 347(2), 397–406.
Vancouver
1.
Verdoodt F, Bert W, Couvreur M, De Mulder K, Willems M. Proliferative response of the stem cell system during regeneration of the rostrum in Macrostomum lignano (Platyhelminthes). CELL AND TISSUE RESEARCH. 2012;347(2):397–406.
MLA
Verdoodt, Freija, Wim Bert, Marjolein Couvreur, et al. “Proliferative Response of the Stem Cell System During Regeneration of the Rostrum in Macrostomum Lignano (Platyhelminthes).” CELL AND TISSUE RESEARCH 347.2 (2012): 397–406. Print.