Ghent University Academic Bibliography

Advanced

L-selectin and beta(2)-integrin expression on circulating bovine polymorphonuclear leukocytes during endotoxin mastitis

Araceli Diez Fraile, Evelyne Meyer UGent, Luc Duchateau UGent and Christian Burvenich UGent (2003) JOURNAL OF DAIRY SCIENCE. 86(7). p.2334-2342
abstract
The aim of this in vivo study was to examine the effect of intramammarily administered endotoxin (lipopolysaccharide, LPS) on the expression of L-selectin (CD62L) and the beta(2)-integrin subunits CD11b and CD18 on circulating bovine PMN. Six early lactating cows were infused with Escherichia coli LPS. The adhesion molecules under study were stained at the cell surface and analyzed flow cytometrically. In addition, some of the clinical parameters associated with adhesion molecule mobilization such as fever, blood cortisol levels, somatic cell count (SCC), and total and differential blood leukocyte count were measured. In analogy with observations during clinical coliform mastitis, a progressive decrease of CD62L expression levels was observed early after LPS infusion, concomitantly with a continuous rise of CD11b and CD18 density. However, no correlation was found between the kinetics of CD11b and CD18 density. The initial changes in adhesion molecule expression paralleled the decrease in blood PMN numbers, together with the increase in rectal temperature, cortisol levels, SCC, and number of circulating immature PMN. In conclusion, intramammarily administered LPS seems to play an important role in modulating adhesion receptor expression on circulating bovine PMN. Interestingly, in contrast to coliform mastitis, the net CD18 variation is not principally influenced by CD11b upregulation during endotoxin administration. The knowledge of adhesion molecule kinetics in relation to the different parameters evaluated in the present study contributes to an improved understanding of the inflammatory reaction.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
adhesion molecule, endotoxin mastitis, polymorphonuclear leukocyte, bovine, ESCHERICHIA-COLI MASTITIS, TUMOR-NECROSIS-FACTOR, ADHESION MOLECULES, STAPHYLOCOCCUS-AUREUS, CYTOKINE PRODUCTION, SURFACE EXPRESSION, MAMMARY-GLAND, DAIRY-COWS, NEUTROPHILS, CORTISOL
journal title
JOURNAL OF DAIRY SCIENCE
J. Dairy Sci.
volume
86
issue
7
pages
2334-2342 pages
Web of Science type
Article
Web of Science id
000183657100009
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
2.139 (2003)
JCR rank
4/94 (2003)
JCR quartile
1 (2003)
ISSN
0022-0302
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
209750
handle
http://hdl.handle.net/1854/LU-209750
alternative location
http://jds.fass.org/cgi/reprint/86/7/2334
date created
2004-04-08 17:43:00
date last changed
2016-12-19 15:38:52
@article{209750,
  abstract     = {The aim of this in vivo study was to examine the effect of intramammarily administered endotoxin (lipopolysaccharide, LPS) on the expression of L-selectin (CD62L) and the beta(2)-integrin subunits CD11b and CD18 on circulating bovine PMN. Six early lactating cows were infused with Escherichia coli LPS. The adhesion molecules under study were stained at the cell surface and analyzed flow cytometrically. In addition, some of the clinical parameters associated with adhesion molecule mobilization such as fever, blood cortisol levels, somatic cell count (SCC), and total and differential blood leukocyte count were measured. In analogy with observations during clinical coliform mastitis, a progressive decrease of CD62L expression levels was observed early after LPS infusion, concomitantly with a continuous rise of CD11b and CD18 density. However, no correlation was found between the kinetics of CD11b and CD18 density. The initial changes in adhesion molecule expression paralleled the decrease in blood PMN numbers, together with the increase in rectal temperature, cortisol levels, SCC, and number of circulating immature PMN. In conclusion, intramammarily administered LPS seems to play an important role in modulating adhesion receptor expression on circulating bovine PMN. Interestingly, in contrast to coliform mastitis, the net CD18 variation is not principally influenced by CD11b upregulation during endotoxin administration. The knowledge of adhesion molecule kinetics in relation to the different parameters evaluated in the present study contributes to an improved understanding of the inflammatory reaction.},
  author       = {Diez Fraile, Araceli and Meyer, Evelyne and Duchateau, Luc and Burvenich, Christian},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keyword      = {adhesion molecule,endotoxin mastitis,polymorphonuclear leukocyte,bovine,ESCHERICHIA-COLI MASTITIS,TUMOR-NECROSIS-FACTOR,ADHESION MOLECULES,STAPHYLOCOCCUS-AUREUS,CYTOKINE PRODUCTION,SURFACE EXPRESSION,MAMMARY-GLAND,DAIRY-COWS,NEUTROPHILS,CORTISOL},
  language     = {eng},
  number       = {7},
  pages        = {2334--2342},
  title        = {L-selectin and beta(2)-integrin expression on circulating bovine polymorphonuclear leukocytes during endotoxin mastitis},
  url          = {http://jds.fass.org/cgi/reprint/86/7/2334},
  volume       = {86},
  year         = {2003},
}

Chicago
Diez Fraile, Araceli, Evelyne Meyer, Luc Duchateau, and Christian Burvenich. 2003. “L-selectin and Beta(2)-integrin Expression on Circulating Bovine Polymorphonuclear Leukocytes During Endotoxin Mastitis.” Journal of Dairy Science 86 (7): 2334–2342.
APA
Diez Fraile, A., Meyer, E., Duchateau, L., & Burvenich, C. (2003). L-selectin and beta(2)-integrin expression on circulating bovine polymorphonuclear leukocytes during endotoxin mastitis. JOURNAL OF DAIRY SCIENCE, 86(7), 2334–2342.
Vancouver
1.
Diez Fraile A, Meyer E, Duchateau L, Burvenich C. L-selectin and beta(2)-integrin expression on circulating bovine polymorphonuclear leukocytes during endotoxin mastitis. JOURNAL OF DAIRY SCIENCE. 2003;86(7):2334–42.
MLA
Diez Fraile, Araceli, Evelyne Meyer, Luc Duchateau, et al. “L-selectin and Beta(2)-integrin Expression on Circulating Bovine Polymorphonuclear Leukocytes During Endotoxin Mastitis.” JOURNAL OF DAIRY SCIENCE 86.7 (2003): 2334–2342. Print.