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Tracking the progression of the human inner cell mass during embryonic stem cell derivation

(2012) NATURE BIOTECHNOLOGY. 30(3). p.278-282
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Abstract
The different pluripotent states of mouse embryonic stem cells (ESCs) in vitro have been shown to correspond to stages of mouse embryonic development(1-6). For human cells, little is known about the events that precede the generation of ESCs or whether they correlate with in vivo developmental stages. Here we investigate the cellular and molecular changes that occur during the transition from the human inner cell mass (ICM) to ESCs in vitro. We demonstrate that human ESCs originate from a post-ICM intermediate (PICMI), a transient epiblast-like structure that has undergone X-inactivation in female cells and is both necessary and sufficient for ESC derivation. The PICMI is the result of progressive and defined ICM organization in vitro and has a distinct state of cell signaling. The PICMI can be cryopreserved without compromising ESC derivation capacity. As a closer progenitor of ESCs than the ICM, the PICMI provides insight into the pluripotent state of human stem cells.
Keywords
STATES, LINES, INACTIVATION, EXPRESSION, EPIBLAST, PLURIPOTENCY, WNT/BETA-CATENIN, FATE DECISIONS, MOUSE EMBRYOS, PRIMORDIAL GERM-CELLS

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Citation

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Chicago
O’Leary, Thomas, Björn Heindryckx, Sylvie Lierman, David van Bruggen, Jelle J Goeman, Mado Vandewoestyne, Dieter Deforce, Susana M Chuva de Sousa Lopes, and Petra De Sutter. 2012. “Tracking the Progression of the Human Inner Cell Mass During Embryonic Stem Cell Derivation.” Nature Biotechnology 30 (3): 278–282.
APA
O’Leary, T., Heindryckx, B., Lierman, S., van Bruggen, D., Goeman, J. J., Vandewoestyne, M., Deforce, D., et al. (2012). Tracking the progression of the human inner cell mass during embryonic stem cell derivation. NATURE BIOTECHNOLOGY, 30(3), 278–282.
Vancouver
1.
O’Leary T, Heindryckx B, Lierman S, van Bruggen D, Goeman JJ, Vandewoestyne M, et al. Tracking the progression of the human inner cell mass during embryonic stem cell derivation. NATURE BIOTECHNOLOGY. 2012;30(3):278–82.
MLA
O’Leary, Thomas, Björn Heindryckx, Sylvie Lierman, et al. “Tracking the Progression of the Human Inner Cell Mass During Embryonic Stem Cell Derivation.” NATURE BIOTECHNOLOGY 30.3 (2012): 278–282. Print.
@article{2096570,
  abstract     = {The different pluripotent states of mouse embryonic stem cells (ESCs) in vitro have been shown to correspond to stages of mouse embryonic development(1-6). For human cells, little is known about the events that precede the generation of ESCs or whether they correlate with in vivo developmental stages. Here we investigate the cellular and molecular changes that occur during the transition from the human inner cell mass (ICM) to ESCs in vitro. We demonstrate that human ESCs originate from a post-ICM intermediate (PICMI), a transient epiblast-like structure that has undergone X-inactivation in female cells and is both necessary and sufficient for ESC derivation. The PICMI is the result of progressive and defined ICM organization in vitro and has a distinct state of cell signaling. The PICMI can be cryopreserved without compromising ESC derivation capacity. As a closer progenitor of ESCs than the ICM, the PICMI provides insight into the pluripotent state of human stem cells.},
  author       = {O'Leary, Thomas and Heindryckx, Bj{\"o}rn and Lierman, Sylvie and van Bruggen, David  and Goeman, Jelle J and Vandewoestyne, Mado and Deforce, Dieter and Chuva de Sousa Lopes, Susana M and De Sutter, Petra},
  issn         = {1087-0156},
  journal      = {NATURE BIOTECHNOLOGY},
  keyword      = {STATES,LINES,INACTIVATION,EXPRESSION,EPIBLAST,PLURIPOTENCY,WNT/BETA-CATENIN,FATE DECISIONS,MOUSE EMBRYOS,PRIMORDIAL GERM-CELLS},
  language     = {eng},
  number       = {3},
  pages        = {278--282},
  title        = {Tracking the progression of the human inner cell mass during embryonic stem cell derivation},
  url          = {http://dx.doi.org/10.1038/nbt.2135},
  volume       = {30},
  year         = {2012},
}

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