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Tracking the progression of the human inner cell mass during embryonic stem cell derivation

Thomas O'Leary UGent, Björn Heindryckx UGent, Sylvie Lierman UGent, David van Bruggen, Jelle J Goeman, Mado Vandewoestyne UGent, Dieter Deforce UGent, Susana M Chuva de Sousa Lopes and Petra De Sutter UGent (2012) NATURE BIOTECHNOLOGY. 30(3). p.278-282
abstract
The different pluripotent states of mouse embryonic stem cells (ESCs) in vitro have been shown to correspond to stages of mouse embryonic development(1-6). For human cells, little is known about the events that precede the generation of ESCs or whether they correlate with in vivo developmental stages. Here we investigate the cellular and molecular changes that occur during the transition from the human inner cell mass (ICM) to ESCs in vitro. We demonstrate that human ESCs originate from a post-ICM intermediate (PICMI), a transient epiblast-like structure that has undergone X-inactivation in female cells and is both necessary and sufficient for ESC derivation. The PICMI is the result of progressive and defined ICM organization in vitro and has a distinct state of cell signaling. The PICMI can be cryopreserved without compromising ESC derivation capacity. As a closer progenitor of ESCs than the ICM, the PICMI provides insight into the pluripotent state of human stem cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
STATES, LINES, INACTIVATION, EXPRESSION, EPIBLAST, PLURIPOTENCY, WNT/BETA-CATENIN, FATE DECISIONS, MOUSE EMBRYOS, PRIMORDIAL GERM-CELLS
journal title
NATURE BIOTECHNOLOGY
Nat. Biotechnol.
volume
30
issue
3
pages
278 - 282
Web of Science type
Article
Web of Science id
000301303800025
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
32.438 (2012)
JCR rank
2/157 (2012)
JCR quartile
1 (2012)
ISSN
1087-0156
DOI
10.1038/nbt.2135
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2096570
handle
http://hdl.handle.net/1854/LU-2096570
date created
2012-04-30 15:49:39
date last changed
2012-05-02 09:12:10
@article{2096570,
  abstract     = {The different pluripotent states of mouse embryonic stem cells (ESCs) in vitro have been shown to correspond to stages of mouse embryonic development(1-6). For human cells, little is known about the events that precede the generation of ESCs or whether they correlate with in vivo developmental stages. Here we investigate the cellular and molecular changes that occur during the transition from the human inner cell mass (ICM) to ESCs in vitro. We demonstrate that human ESCs originate from a post-ICM intermediate (PICMI), a transient epiblast-like structure that has undergone X-inactivation in female cells and is both necessary and sufficient for ESC derivation. The PICMI is the result of progressive and defined ICM organization in vitro and has a distinct state of cell signaling. The PICMI can be cryopreserved without compromising ESC derivation capacity. As a closer progenitor of ESCs than the ICM, the PICMI provides insight into the pluripotent state of human stem cells.},
  author       = {O'Leary, Thomas and Heindryckx, Bj{\"o}rn and Lierman, Sylvie and van Bruggen, David  and Goeman, Jelle J and Vandewoestyne, Mado and Deforce, Dieter and Chuva de Sousa Lopes, Susana M and De Sutter, Petra},
  issn         = {1087-0156},
  journal      = {NATURE BIOTECHNOLOGY},
  keyword      = {STATES,LINES,INACTIVATION,EXPRESSION,EPIBLAST,PLURIPOTENCY,WNT/BETA-CATENIN,FATE DECISIONS,MOUSE EMBRYOS,PRIMORDIAL GERM-CELLS},
  language     = {eng},
  number       = {3},
  pages        = {278--282},
  title        = {Tracking the progression of the human inner cell mass during embryonic stem cell derivation},
  url          = {http://dx.doi.org/10.1038/nbt.2135},
  volume       = {30},
  year         = {2012},
}

Chicago
O’Leary, Thomas, Björn Heindryckx, Sylvie Lierman, David van Bruggen, Jelle J Goeman, Mado Vandewoestyne, Dieter Deforce, Susana M Chuva de Sousa Lopes, and Petra De Sutter. 2012. “Tracking the Progression of the Human Inner Cell Mass During Embryonic Stem Cell Derivation.” Nature Biotechnology 30 (3): 278–282.
APA
O’Leary, T., Heindryckx, B., Lierman, S., van Bruggen, D., Goeman, J. J., Vandewoestyne, M., Deforce, D., et al. (2012). Tracking the progression of the human inner cell mass during embryonic stem cell derivation. NATURE BIOTECHNOLOGY, 30(3), 278–282.
Vancouver
1.
O’Leary T, Heindryckx B, Lierman S, van Bruggen D, Goeman JJ, Vandewoestyne M, et al. Tracking the progression of the human inner cell mass during embryonic stem cell derivation. NATURE BIOTECHNOLOGY. 2012;30(3):278–82.
MLA
O’Leary, Thomas, Björn Heindryckx, Sylvie Lierman, et al. “Tracking the Progression of the Human Inner Cell Mass During Embryonic Stem Cell Derivation.” NATURE BIOTECHNOLOGY 30.3 (2012): 278–282. Print.