Ghent University Academic Bibliography

Advanced

Diatomaceous earth as a protective vehicle for bacteria applied for self-healing concrete

Jianyun Wang UGent, Nele De Belie UGent and Willy Verstraete UGent (2012) JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY. 39(4). p.567-577
abstract
Crack repair is crucial since cracks are the main cause for the decreased service life of concrete structures. An original and promising way to repair cracks is to pre-incorporate healing agents inside the concrete matrix to heal cracks the moment they appear. Thus, the concrete obtains self-healing properties. The goal of our research is to apply bacterially precipitated CaCO3 to heal cracks in concrete since the microbial calcium carbonate is more compatible with the concrete matrix and more environmentally friendly relative to the normally used polymeric materials. Diatomaceous earth (DE) was used in this study to protect bacteria from the high-pH environment of concrete. The experimental results showed that DE had a very good protective effect for bacteria. DE immobilized bacteria had much higher ureolytic activity (12-17 g/l urea was decomposed within 3 days) than that of un-immobilized bacteria (less than 1 g/l urea was decomposed within the same time span) in cement slurry. The optimal concentration of DE for immobilization was 60% (w/v, weight of DE/volume of bacterial suspension). Self-healing in cracked specimens was visualized under light microscopy. The images showed that cracks with a width ranging from 0.15 to 0.17 mm in the specimens containing DE immobilized bacteria were completely filled by the precipitation. Scanning electron microscopy (SEM) and energy dispersive spectrometry (EDS) were used to characterize the precipitation around the crack wall, which was confirmed to be calcium carbonate. The result from a capillary water absorption test showed that the specimens with DE immobilized bacteria had the lowest water absorption (30% of the reference ones), which indicated that the precipitation inside the cracks increased the water penetration resistance of the cracked specimens.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Bacillus sphaericus, Ureolytic activity, Microbial CaCO3, Carrier, Crack repair, CARBONATE PRECIPITATION, LIMESTONE, CEMENT, SYSTEM
journal title
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
J. Ind. Microbiol. Biotechnol.
volume
39
issue
4
pages
567 - 577
Web of Science type
Article
Web of Science id
000302075300006
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
2.321 (2012)
JCR rank
67/157 (2012)
JCR quartile
2 (2012)
ISSN
1367-5435
DOI
10.1007/s10295-011-1037-1
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2095780
handle
http://hdl.handle.net/1854/LU-2095780
date created
2012-04-27 10:36:57
date last changed
2014-05-26 10:02:42
@article{2095780,
  abstract     = {Crack repair is crucial since cracks are the main cause for the decreased service life of concrete structures. An original and promising way to repair cracks is to pre-incorporate healing agents inside the concrete matrix to heal cracks the moment they appear. Thus, the concrete obtains self-healing properties. The goal of our research is to apply bacterially precipitated CaCO3 to heal cracks in concrete since the microbial calcium carbonate is more compatible with the concrete matrix and more environmentally friendly relative to the normally used polymeric materials. Diatomaceous earth (DE) was used in this study to protect bacteria from the high-pH environment of concrete. The experimental results showed that DE had a very good protective effect for bacteria. DE immobilized bacteria had much higher ureolytic activity (12-17 g/l urea was decomposed within 3 days) than that of un-immobilized bacteria (less than 1 g/l urea was decomposed within the same time span) in cement slurry. The optimal concentration of DE for immobilization was 60\% (w/v, weight of DE/volume of bacterial suspension). Self-healing in cracked specimens was visualized under light microscopy. The images showed that cracks with a width ranging from 0.15 to 0.17 mm in the specimens containing DE immobilized bacteria were completely filled by the precipitation. Scanning electron microscopy (SEM) and energy dispersive spectrometry (EDS) were used to characterize the precipitation around the crack wall, which was confirmed to be calcium carbonate. The result from a capillary water absorption test showed that the specimens with DE immobilized bacteria had the lowest water absorption (30\% of the reference ones), which indicated that the precipitation inside the cracks increased the water penetration resistance of the cracked specimens.},
  author       = {Wang, Jianyun and De Belie, Nele and Verstraete, Willy},
  issn         = {1367-5435},
  journal      = {JOURNAL OF INDUSTRIAL MICROBIOLOGY \& BIOTECHNOLOGY},
  keyword      = {Bacillus sphaericus,Ureolytic activity,Microbial CaCO3,Carrier,Crack repair,CARBONATE PRECIPITATION,LIMESTONE,CEMENT,SYSTEM},
  language     = {eng},
  number       = {4},
  pages        = {567--577},
  title        = {Diatomaceous earth as a protective vehicle for bacteria applied for self-healing concrete},
  url          = {http://dx.doi.org/10.1007/s10295-011-1037-1},
  volume       = {39},
  year         = {2012},
}

Chicago
Wang, Jianyun, Nele De Belie, and Willy Verstraete. 2012. “Diatomaceous Earth as a Protective Vehicle for Bacteria Applied for Self-healing Concrete.” Journal of Industrial Microbiology & Biotechnology 39 (4): 567–577.
APA
Wang, Jianyun, De Belie, N., & Verstraete, W. (2012). Diatomaceous earth as a protective vehicle for bacteria applied for self-healing concrete. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 39(4), 567–577.
Vancouver
1.
Wang J, De Belie N, Verstraete W. Diatomaceous earth as a protective vehicle for bacteria applied for self-healing concrete. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY. 2012;39(4):567–77.
MLA
Wang, Jianyun, Nele De Belie, and Willy Verstraete. “Diatomaceous Earth as a Protective Vehicle for Bacteria Applied for Self-healing Concrete.” JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 39.4 (2012): 567–577. Print.