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High frequency of single-copy T-DNA transformants produced after floral dip in CRE-expressing Arabidopsis plants

Annelies De Paepe UGent, Sylvie De Buck UGent, Jonah Nolf UGent and Anna Depicker UGent (2012) Transgenic plants : methods and protocols. In Methods in Molecular Biology 847. p.317-333
abstract
Transgenic plants that harbor a single copy of the introduced transgene are preferable to those with multiple transgene copies because multiple T-DNA copies correlate with expression variability and susceptibility to silencing. Especially after the commonly used fl oral-dip Agrobacterium -mediated transformation method, the frequency of single-copy transformants is low. The CRE/ loxP recombinase-based strategy to resolve complex T-DNA loci has proven to be successful to effi ciently obtain single-copy T-DNA transformants by directly transforming loxP -containing T-DNA vectors in CRE -expressing Arabidopsis thaliana plants. This chapter describes in detail how to transform three available loxP -containing T-DNA vectors into CRE - producing Arabidopsis C24 plants and subsequently how to analyze the transgenic plants for the T-DNA locus structure.
Please use this url to cite or link to this publication:
author
organization
year
type
bookChapter
publication status
published
subject
book title
Transgenic plants : methods and protocols
editor
Jim M Dunwell and Andy C Wetten
series title
Methods in Molecular Biology
volume
847
edition
2nd ed.
pages
317 - 333
publisher
Springer
place of publication
New York, NY, USA
ISBN
9781617795572
9781617795589
DOI
10.1007/978-1-61779-558-9_26
language
English
UGent publication?
yes
classification
B2
copyright statement
I have transferred the copyright for this publication to the publisher
id
2094448
handle
http://hdl.handle.net/1854/LU-2094448
date created
2012-04-25 15:14:37
date last changed
2012-04-26 09:16:30
@incollection{2094448,
  abstract     = {Transgenic plants that harbor a single copy of the introduced transgene are preferable to those with multiple transgene copies because multiple T-DNA copies correlate with expression variability and susceptibility to silencing. Especially after the commonly used fl oral-dip Agrobacterium -mediated transformation method, the frequency of single-copy transformants is low. The CRE/ loxP recombinase-based strategy to resolve complex T-DNA loci has proven to be successful to effi ciently obtain single-copy T-DNA transformants by directly transforming loxP -containing T-DNA vectors in CRE -expressing Arabidopsis thaliana plants. This chapter describes in detail how to transform three available loxP -containing T-DNA vectors into CRE - producing Arabidopsis C24 plants and subsequently how to analyze the transgenic plants for the T-DNA locus structure.},
  author       = {De Paepe, Annelies and De Buck, Sylvie and Nolf, Jonah and Depicker, Anna},
  booktitle    = {Transgenic plants : methods and protocols},
  editor       = {Dunwell, Jim  M and Wetten, Andy C},
  isbn         = {9781617795572},
  language     = {eng},
  pages        = {317--333},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {High frequency of single-copy T-DNA transformants produced after floral dip in CRE-expressing Arabidopsis plants},
  url          = {http://dx.doi.org/10.1007/978-1-61779-558-9\_26},
  volume       = {847},
  year         = {2012},
}

Chicago
De Paepe, Annelies, Sylvie De Buck, Jonah Nolf, and Anna Depicker. 2012. “High Frequency of Single-copy T-DNA Transformants Produced After Floral Dip in CRE-expressing Arabidopsis Plants.” In Transgenic Plants : Methods and Protocols, ed. Jim  M Dunwell and Andy C Wetten, 847:317–333. 2nd ed. New York, NY, USA: Springer.
APA
De Paepe, Annelies, De Buck, S., Nolf, J., & Depicker, A. (2012). High frequency of single-copy T-DNA transformants produced after floral dip in CRE-expressing Arabidopsis plants. In J. M. Dunwell & A. C. Wetten (Eds.), Transgenic plants : methods and protocols (2nd ed., Vol. 847, pp. 317–333). New York, NY, USA: Springer.
Vancouver
1.
De Paepe A, De Buck S, Nolf J, Depicker A. High frequency of single-copy T-DNA transformants produced after floral dip in CRE-expressing Arabidopsis plants. In: Dunwell JM, Wetten AC, editors. Transgenic plants : methods and protocols. 2nd ed. New York, NY, USA: Springer; 2012. p. 317–33.
MLA
De Paepe, Annelies, Sylvie De Buck, Jonah Nolf, et al. “High Frequency of Single-copy T-DNA Transformants Produced After Floral Dip in CRE-expressing Arabidopsis Plants.” Transgenic Plants : Methods and Protocols. 2nd ed. Ed. Jim  M Dunwell & Andy C Wetten. Vol. 847. New York, NY, USA: Springer, 2012. 317–333. Print.