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Vaccination reduces macrophage infiltration in bronchus-associated lymphoid tissue in pigs infected with a highly virulent Mycoplasma hyopneumoniae strain

Katleen Vranckx UGent, Dominiek Maes UGent, Silvana Beutinger Marchioro UGent, Iris Villarreal UGent, Koen Chiers UGent, Frank Pasmans UGent and Freddy Haesebrouck UGent (2012) BMC VETERINARY RESEARCH. 8.
abstract
Background : Mycoplasma hyopneumoniae is the causative agent of enzootic pneumonia and is responsible for significant economic losses to the pig industry. To better understand the mode of action of a commercial, adjuvanted, inactivated whole cell vaccine and the influence of diversity on the efficacy of vaccination, we investigated samples from vaccinated and non-vaccinated pigs experimentally infected with either a low (LV) or a highly virulent (HV) M. hyopneumoniae strain. Non-vaccinated and sham-infected control groups were included. Lung tissue samples collected at 4 and 8 weeks post infection (PI) were immunohistochemically tested for the presence of T-lymphocytes, B-lymphocytes and macrophages in the bronchus-associated lymphoid tissue (BALT). The number of M. hyopneumoniae organisms in bronchoalveolar lavage (BAL) fluid was determined using quantitative PCR at 4 and 8 weeks PI. Serum antibodies against M. hyopneumoniae were determined at 0, 2, 4, 6 and 8 weeks PI. Results : The immunostaining revealed a lower density of macrophages in the BALT of the vaccinated groups compared to the non-vaccinated groups. The highest number of M. hyopneumoniae organisms in the BAL fluid was measured at 4 weeks PI for the HV strain and at 8 weeks PI for the LV strain. Vaccination reduced the number of organisms non-significantly, though for the HV strain the reduction was clinically more relevant than for the LV strain. At the level of the individual pigs, a higher lung lesion score was associated with more M. hyopneumoniae organisms in the lungs and a higher density of the investigated immune cells in the BALT. Conclusions : In conclusion, the infiltration of macrophages after infection with M. hyopneumoniae is reduced by vaccination. The M. hyopneumoniae replication in the lungs is also reduced in vaccinated pigs, though the HV strain is inhibited more than the LV strain.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SERUM, IMMUNE-RESPONSES, IDENTIFICATION, INTERLEUKIN-6, PROTECTION, VACCINES, SYSTEM, REGION, SWINE
journal title
BMC VETERINARY RESEARCH
BMC Vet. Res.
volume
8
article_number
24
pages
7 pages
Web of Science type
Article
Web of Science id
000304030600001
JCR category
VETERINARY SCIENCES
JCR impact factor
1.861 (2012)
JCR rank
18/142 (2012)
JCR quartile
1 (2012)
ISSN
1746-6148
DOI
10.1186/1746-6148-8-24
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2088375
handle
http://hdl.handle.net/1854/LU-2088375
date created
2012-04-17 14:02:10
date last changed
2012-09-11 16:23:52
@article{2088375,
  abstract     = {Background : Mycoplasma hyopneumoniae is the causative agent of enzootic pneumonia and is responsible for significant economic losses to the pig industry. To better understand the mode of action of a commercial, adjuvanted, inactivated whole cell vaccine and the influence of diversity on the efficacy of vaccination, we investigated samples from vaccinated and non-vaccinated pigs experimentally infected with either a low (LV) or a highly virulent (HV) M. hyopneumoniae strain. Non-vaccinated and sham-infected control groups were included. Lung tissue samples collected at 4 and 8 weeks post infection (PI) were immunohistochemically tested for the presence of T-lymphocytes, B-lymphocytes and macrophages in the bronchus-associated lymphoid tissue (BALT). The number of M. hyopneumoniae organisms in bronchoalveolar lavage (BAL) fluid was determined using quantitative PCR at 4 and 8 weeks PI. Serum antibodies against M. hyopneumoniae were determined at 0, 2, 4, 6 and 8 weeks PI.
Results : The immunostaining revealed a lower density of macrophages in the BALT of the vaccinated groups compared to the non-vaccinated groups. The highest number of M. hyopneumoniae organisms in the BAL fluid was measured at 4 weeks PI for the HV strain and at 8 weeks PI for the LV strain. Vaccination reduced the number of organisms non-significantly, though for the HV strain the reduction was clinically more relevant than for the LV strain. At the level of the individual pigs, a higher lung lesion score was associated with more M. hyopneumoniae organisms in the lungs and a higher density of the investigated immune cells in the BALT.
Conclusions : In conclusion, the infiltration of macrophages after infection with M. hyopneumoniae is reduced by vaccination. The M. hyopneumoniae replication in the lungs is also reduced in vaccinated pigs, though the HV strain is inhibited more than the LV strain.},
  articleno    = {24},
  author       = {Vranckx, Katleen and Maes, Dominiek and Beutinger Marchioro, Silvana and Villarreal, Iris and Chiers, Koen and Pasmans, Frank and Haesebrouck, Freddy},
  issn         = {1746-6148},
  journal      = {BMC VETERINARY RESEARCH},
  keyword      = {SERUM,IMMUNE-RESPONSES,IDENTIFICATION,INTERLEUKIN-6,PROTECTION,VACCINES,SYSTEM,REGION,SWINE},
  language     = {eng},
  pages        = {7},
  title        = {Vaccination reduces macrophage infiltration in bronchus-associated lymphoid tissue in pigs infected with a highly virulent Mycoplasma hyopneumoniae strain},
  url          = {http://dx.doi.org/10.1186/1746-6148-8-24},
  volume       = {8},
  year         = {2012},
}

Chicago
Vranckx, Katleen, Dominiek Maes, Silvana Beutinger Marchioro, Iris Villarreal, Koen Chiers, Frank Pasmans, and Freddy Haesebrouck. 2012. “Vaccination Reduces Macrophage Infiltration in Bronchus-associated Lymphoid Tissue in Pigs Infected with a Highly Virulent Mycoplasma Hyopneumoniae Strain.” Bmc Veterinary Research 8.
APA
Vranckx, K., Maes, D., Beutinger Marchioro, S., Villarreal, I., Chiers, K., Pasmans, F., & Haesebrouck, F. (2012). Vaccination reduces macrophage infiltration in bronchus-associated lymphoid tissue in pigs infected with a highly virulent Mycoplasma hyopneumoniae strain. BMC VETERINARY RESEARCH, 8.
Vancouver
1.
Vranckx K, Maes D, Beutinger Marchioro S, Villarreal I, Chiers K, Pasmans F, et al. Vaccination reduces macrophage infiltration in bronchus-associated lymphoid tissue in pigs infected with a highly virulent Mycoplasma hyopneumoniae strain. BMC VETERINARY RESEARCH. 2012;8.
MLA
Vranckx, Katleen, Dominiek Maes, Silvana Beutinger Marchioro, et al. “Vaccination Reduces Macrophage Infiltration in Bronchus-associated Lymphoid Tissue in Pigs Infected with a Highly Virulent Mycoplasma Hyopneumoniae Strain.” BMC VETERINARY RESEARCH 8 (2012): n. pag. Print.