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A comprehensive analysis of genes encoding small secreted proteins identifies candidate effectors in Melampsora larici-populina (poplar leaf rust)

Stéphane Hacquard, David L Joly, Yao-Cheng Lin UGent, Emilie Tisserant, Nicolas Feau, Christine Delaruelle, Valérie Legué, Annegret Kohler, Philippe Tanguay and Benjamin Petre, et al. (2012) MOLECULAR PLANT-MICROBE INTERACTIONS. 25(3). p.279-293
abstract
The obligate biotrophic rust fungus Melampsora larici-populina is the most devastating and widespread pathogen of poplars. Studies over recent years have identified various small secreted proteins (SSP) from plant biotrophic filamentous pathogens and have highlighted their role as effectors in host-pathogen interactions. The recent analysis of the M. larici-populina genome sequence has revealed the presence of 1,184 SSP-encoding genes in this rust fungus. In the present study, the expression and evolutionary dynamics of these SSP were investigated to pinpoint the arsenal of putative effectors that could be involved in the interaction between the rust fungus and poplar. Similarity with effectors previously described in Melampsora spp., richness in cysteines, and organization in large families were extensively detailed and discussed. Positive selection analyses conducted over clusters of paralogous genes revealed fast-evolving candidate effectors. Transcript profiling of selected M. laricipopulina SSP showed a timely coordinated expression during leaf infection, and the accumulation of four candidate effectors in distinct rust infection structures was demonstrated by immunolocalization. This integrated and multifaceted approach helps to prioritize candidate effector genes for functional studies
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
LACCARIA-BICOLOR, POSITIVE SELECTION, OBLIGATE BIOTROPHY, AVIRULENCE PROTEINS, CLADOSPORIUM-FULVUM, PATHOGEN INTERACTIONS, MULTIPLE SEQUENCE ALIGNMENT, PLANT-CELLS, FLAX RUST, RESISTANCE GENES
journal title
MOLECULAR PLANT-MICROBE INTERACTIONS
Mol. Plant-Microbe Interact.
volume
25
issue
3
pages
279 - 293
Web of Science type
Article
Web of Science id
000300043400002
JCR category
PLANT SCIENCES
JCR impact factor
4.307 (2012)
JCR rank
17/193 (2012)
JCR quartile
1 (2012)
ISSN
0894-0282
DOI
10.1094/MPMI-09-11-0238
project
Bioinformatics: from nucleotids to networks (N2N)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2087559
handle
http://hdl.handle.net/1854/LU-2087559
date created
2012-04-16 15:42:51
date last changed
2013-05-06 11:10:00
@article{2087559,
  abstract     = {The obligate biotrophic rust fungus Melampsora larici-populina is the most devastating and widespread pathogen of poplars. Studies over recent years have identified various small secreted proteins (SSP) from plant biotrophic filamentous pathogens and have highlighted their role as effectors in host-pathogen interactions. The recent analysis of the M. larici-populina genome sequence has revealed the presence of 1,184 SSP-encoding genes in this rust fungus. In the present study, the expression and evolutionary dynamics of these SSP were investigated to pinpoint the arsenal of putative effectors that could be involved in the interaction between the rust fungus and poplar. Similarity with effectors previously described in Melampsora spp., richness in cysteines, and organization in large families were extensively detailed and discussed. Positive selection analyses conducted over clusters of paralogous genes revealed fast-evolving candidate effectors. Transcript profiling of selected M. laricipopulina SSP showed a timely coordinated expression during leaf infection, and the accumulation of four candidate effectors in distinct rust infection structures was demonstrated by immunolocalization. This integrated and multifaceted approach helps to prioritize candidate effector genes for functional studies},
  author       = {Hacquard, St{\'e}phane and Joly, David L and Lin, Yao-Cheng and Tisserant, Emilie and Feau, Nicolas and Delaruelle, Christine and Legu{\'e}, Val{\'e}rie and Kohler, Annegret and Tanguay, Philippe and Petre, Benjamin and Frey, Pascal and Van de Peer, Yves and Rouz{\'e}, Pierre and Martin, Francis and Hamelin, Richard C and Duplessis, S{\'e}bastien},
  issn         = {0894-0282},
  journal      = {MOLECULAR PLANT-MICROBE INTERACTIONS},
  keyword      = {LACCARIA-BICOLOR,POSITIVE SELECTION,OBLIGATE BIOTROPHY,AVIRULENCE PROTEINS,CLADOSPORIUM-FULVUM,PATHOGEN INTERACTIONS,MULTIPLE SEQUENCE ALIGNMENT,PLANT-CELLS,FLAX RUST,RESISTANCE GENES},
  language     = {eng},
  number       = {3},
  pages        = {279--293},
  title        = {A comprehensive analysis of genes encoding small secreted proteins identifies candidate effectors in Melampsora larici-populina (poplar leaf rust)},
  url          = {http://dx.doi.org/10.1094/MPMI-09-11-0238},
  volume       = {25},
  year         = {2012},
}

Chicago
Hacquard, Stéphane, David L Joly, Yao-Cheng Lin, Emilie Tisserant, Nicolas Feau, Christine Delaruelle, Valérie Legué, et al. 2012. “A Comprehensive Analysis of Genes Encoding Small Secreted Proteins Identifies Candidate Effectors in Melampsora Larici-populina (poplar Leaf Rust).” Molecular Plant-microbe Interactions 25 (3): 279–293.
APA
Hacquard, S., Joly, D. L., Lin, Y.-C., Tisserant, E., Feau, N., Delaruelle, C., Legué, V., et al. (2012). A comprehensive analysis of genes encoding small secreted proteins identifies candidate effectors in Melampsora larici-populina (poplar leaf rust). MOLECULAR PLANT-MICROBE INTERACTIONS, 25(3), 279–293.
Vancouver
1.
Hacquard S, Joly DL, Lin Y-C, Tisserant E, Feau N, Delaruelle C, et al. A comprehensive analysis of genes encoding small secreted proteins identifies candidate effectors in Melampsora larici-populina (poplar leaf rust). MOLECULAR PLANT-MICROBE INTERACTIONS. 2012;25(3):279–93.
MLA
Hacquard, Stéphane, David L Joly, Yao-Cheng Lin, et al. “A Comprehensive Analysis of Genes Encoding Small Secreted Proteins Identifies Candidate Effectors in Melampsora Larici-populina (poplar Leaf Rust).” MOLECULAR PLANT-MICROBE INTERACTIONS 25.3 (2012): 279–293. Print.