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Root gravitropism is regulated by a transient lateral auxin gradient controlled by a tipping-point mechanism

Leah R Band, Darren M Wells, Antoine Larrieu, Jianyong Sun, Alistair M Middleton, Andrew P French, Géraldine Brunoud, Ethel Mendocilla Sato, Michael H Wilson, Benjamin Péret, et al. (2012) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 109(12). p.4668-4673
abstract
Gravity profoundly influences plant growth and development. Plants respond to changes in orientation by using gravitropic responses to modify their growth. Cholodny and Went hypothesized over 80 years ago that plants bend in response to a gravity stimulus by generating a lateral gradient of a growth regulator at an organ's apex, later found to be auxin. Auxin regulates root growth by targeting Aux/IAA repressor proteins for degradation. We used an Aux/IAA-based reporter, domain II (DII)-VENUS, in conjunction with a mathematical model to quantify auxin redistribution following a gravity stimulus. Our multidisciplinary approach revealed that auxin is rapidly redistributed to the lower side of the root within minutes of a 90 degrees gravity stimulus. Unexpectedly, auxin asymmetry was rapidly lost as bending root tips reached an angle of 40 degrees to the horizontal. We hypothesize roots use a "tipping point" mechanism that operates to reverse the asymmetric auxin flow at the midpoint of root bending. These mechanistic insights illustrate the scientific value of developing quantitative reporters such as DII-VENUS in conjunction with parameterized mathematical models to provide high-resolution kinetics of hormone redistribution.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARABIDOPSIS, TRANSPORT, GRAVITY, GENE-EXPRESSION, AUX/IAA PROTEINS, ENDOPLASMIC-RETICULUM, SEDIMENTATION KINETICS, BOX PROTEIN TIR1, GROWTH, CELLS
journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Proc. Natl. Acad. Sci. USA
volume
109
issue
12
pages
4668 - 4673
Web of Science type
Article
Web of Science id
000301712600064
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
9.737 (2012)
JCR rank
4/56 (2012)
JCR quartile
1 (2012)
ISSN
0027-8424
DOI
10.1073/pnas.1201498109
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2087419
handle
http://hdl.handle.net/1854/LU-2087419
date created
2012-04-16 15:24:41
date last changed
2016-12-19 15:38:12
@article{2087419,
  abstract     = {Gravity profoundly influences plant growth and development. Plants respond to changes in orientation by using gravitropic responses to modify their growth. Cholodny and Went hypothesized over 80 years ago that plants bend in response to a gravity stimulus by generating a lateral gradient of a growth regulator at an organ's apex, later found to be auxin. Auxin regulates root growth by targeting Aux/IAA repressor proteins for degradation. We used an Aux/IAA-based reporter, domain II (DII)-VENUS, in conjunction with a mathematical model to quantify auxin redistribution following a gravity stimulus. Our multidisciplinary approach revealed that auxin is rapidly redistributed to the lower side of the root within minutes of a 90 degrees gravity stimulus. Unexpectedly, auxin asymmetry was rapidly lost as bending root tips reached an angle of 40 degrees to the horizontal. We hypothesize roots use a {\textacutedbl}tipping point{\textacutedbl} mechanism that operates to reverse the asymmetric auxin flow at the midpoint of root bending. These mechanistic insights illustrate the scientific value of developing quantitative reporters such as DII-VENUS in conjunction with parameterized mathematical models to provide high-resolution kinetics of hormone redistribution.},
  author       = {Band, Leah R and Wells, Darren M and Larrieu, Antoine and Sun, Jianyong and Middleton, Alistair M and French, Andrew P and Brunoud, G{\'e}raldine and Sato, Ethel Mendocilla and Wilson, Michael H and P{\'e}ret, Benjamin and Oliva, Marina and Swarup, Ranjan and Sairanen, Ilkka and Parry, Geraint and Ljung, Karin and Beeckman, Tom and Garibaldi, Jonathan M and Estelle, Mark and Owen, Markus R and Vissenberg, Kris and Hodgman, T Charlie and Pridmore, Tony P and King, John R and Vernoux, Teva and Bennett, Malcolm J},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {ARABIDOPSIS,TRANSPORT,GRAVITY,GENE-EXPRESSION,AUX/IAA PROTEINS,ENDOPLASMIC-RETICULUM,SEDIMENTATION KINETICS,BOX PROTEIN TIR1,GROWTH,CELLS},
  language     = {eng},
  number       = {12},
  pages        = {4668--4673},
  title        = {Root gravitropism is regulated by a transient lateral auxin gradient controlled by a tipping-point mechanism},
  url          = {http://dx.doi.org/10.1073/pnas.1201498109},
  volume       = {109},
  year         = {2012},
}

Chicago
Band, Leah R, Darren M Wells, Antoine Larrieu, Jianyong Sun, Alistair M Middleton, Andrew P French, Géraldine Brunoud, et al. 2012. “Root Gravitropism Is Regulated by a Transient Lateral Auxin Gradient Controlled by a Tipping-point Mechanism.” Proceedings of the National Academy of Sciences of the United States of America 109 (12): 4668–4673.
APA
Band, L. R., Wells, D. M., Larrieu, A., Sun, J., Middleton, A. M., French, A. P., Brunoud, G., et al. (2012). Root gravitropism is regulated by a transient lateral auxin gradient controlled by a tipping-point mechanism. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(12), 4668–4673.
Vancouver
1.
Band LR, Wells DM, Larrieu A, Sun J, Middleton AM, French AP, et al. Root gravitropism is regulated by a transient lateral auxin gradient controlled by a tipping-point mechanism. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2012;109(12):4668–73.
MLA
Band, Leah R, Darren M Wells, Antoine Larrieu, et al. “Root Gravitropism Is Regulated by a Transient Lateral Auxin Gradient Controlled by a Tipping-point Mechanism.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109.12 (2012): 4668–4673. Print.