Ghent University Academic Bibliography

Advanced

Changes in the pre-parasitic developmental stage of Globodera rostochiensis in response to green manures

Yirina Valdés Vázquez UGent, Nicole Viaene, Vivian Blok, Juan E Palomares-Rius and Maurice Moens UGent (2012) NEMATOLOGY. 14. p.925-932
abstract
The sequence of events during the pre-parasitic and parasitic stages of the life cycle of Globodera rostochiensis, starting from hatching until host invasion and infection, is of major importance. Many of the physiological changes are initiated in the preparasitic stage in response to the influences exerted by the root diffusates of the host plant. The objectives of this study were to investigate whether root diffusates and extracts of green manures from Brassicaceae, in particular yellow mustard, fodder radish and rapeseed, have a direct effect on the pre-parasitic stage of G. rostochiensis. Unhatched second-stage juveniles (J2) were exposed to root diffusates and extracts from roots or above-ground parts of the green manures to assess: i) changes in eggshell permeability; ii) changes in diameter of the dorsal gland nucleolus; and iii) the activation of transcription in the dorsal and subventral pharyngeal glands. The 24 h exposure of unhatched J2 to the green manures did not indicate an alteration in eggshell permeability. The diameter of the dorsal gland nucleolus increased significantly after exposure to diffusates but not to extracts of the green manures, although the size in that case still did not exceed the measurements in J2 exposed to tomato root diffusates. The expression of the gene IVg9 in the dorsal gland was not detected after any treatment. However, transcriptional activation in the subventral pharyngeal glands was observed. Although the maximum expression of the cellulase gene was found in J2 exposed to tomato root diffusates, the expression in J2 exposed to root extracts of green manures was higher than in J2 exposed to root diffusates and extracts of above-ground plant parts. The potential of the investigated brassicaceous green manures to influence the pre-parasitic stage of G. rostochiensis is discussed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
root diffusate, transcription, potato cyst nematode, plant extracts, pharyngeal gland, IVg9 gene, hatching, eggshell permeability, cellulase gene, POTATO CYST-NEMATODE, HETERODERA-GLYCINES EGGS, GENE-EXPRESSION, ROOT DIFFUSATE, PALLIDA, IDENTIFICATION, JUVENILES, SPP.
journal title
NEMATOLOGY
Nematology
volume
14
pages
925 - 932
Web of Science type
Article
Web of Science id
000312612200003
JCR category
ZOOLOGY
JCR impact factor
0.914 (2012)
JCR rank
86/149 (2012)
JCR quartile
3 (2012)
ISSN
1388-5545
DOI
10.1163/156854112X635869
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2086950
handle
http://hdl.handle.net/1854/LU-2086950
date created
2012-04-16 09:45:20
date last changed
2013-07-12 11:57:04
@article{2086950,
  abstract     = {The sequence of events during the pre-parasitic and parasitic stages of the life cycle of Globodera rostochiensis, starting from hatching until host invasion and infection, is of major importance. Many of the physiological changes are initiated in the preparasitic stage in response to the influences exerted by the root diffusates of the host plant. The objectives of this study were to investigate whether root diffusates and extracts of green manures from Brassicaceae, in particular yellow mustard, fodder radish and rapeseed, have a direct effect on the pre-parasitic stage of G. rostochiensis. Unhatched second-stage juveniles (J2) were exposed to root diffusates and extracts from roots or above-ground parts of the green manures to assess: i) changes in eggshell permeability; ii) changes in diameter of the dorsal gland nucleolus; and iii) the activation of transcription in the dorsal and subventral pharyngeal glands. The 24 h exposure of unhatched J2 to the green manures did not indicate an alteration in eggshell permeability. The diameter of the dorsal gland nucleolus increased significantly after exposure to diffusates but not to extracts of the green manures, although the size in that case still did not exceed the measurements in J2 exposed to tomato root diffusates. The expression of the gene IVg9 in the dorsal gland was not detected after any treatment. However, transcriptional activation in the subventral pharyngeal glands was observed. Although the maximum expression of the cellulase gene was found in J2 exposed to tomato root diffusates, the expression in J2 exposed to root extracts of green manures was higher than in J2 exposed to root diffusates and extracts of above-ground plant parts. The potential of the investigated brassicaceous green manures to influence the pre-parasitic stage of G. rostochiensis is discussed.},
  author       = {Vald{\'e}s V{\'a}zquez, Yirina and Viaene, Nicole and Blok, Vivian and Palomares-Rius, Juan E and Moens, Maurice},
  issn         = {1388-5545},
  journal      = {NEMATOLOGY},
  keyword      = {root diffusate,transcription,potato cyst nematode,plant extracts,pharyngeal gland,IVg9 gene,hatching,eggshell permeability,cellulase gene,POTATO CYST-NEMATODE,HETERODERA-GLYCINES EGGS,GENE-EXPRESSION,ROOT DIFFUSATE,PALLIDA,IDENTIFICATION,JUVENILES,SPP.},
  language     = {eng},
  pages        = {925--932},
  title        = {Changes in the pre-parasitic developmental stage of Globodera rostochiensis in response to green manures},
  url          = {http://dx.doi.org/10.1163/156854112X635869},
  volume       = {14},
  year         = {2012},
}

Chicago
Valdés Vázquez, Yirina, Nicole Viaene, Vivian Blok, Juan E Palomares-Rius, and Maurice Moens. 2012. “Changes in the Pre-parasitic Developmental Stage of Globodera Rostochiensis in Response to Green Manures.” Nematology 14: 925–932.
APA
Valdés Vázquez, Y., Viaene, N., Blok, V., Palomares-Rius, J. E., & Moens, M. (2012). Changes in the pre-parasitic developmental stage of Globodera rostochiensis in response to green manures. NEMATOLOGY, 14, 925–932.
Vancouver
1.
Valdés Vázquez Y, Viaene N, Blok V, Palomares-Rius JE, Moens M. Changes in the pre-parasitic developmental stage of Globodera rostochiensis in response to green manures. NEMATOLOGY. 2012;14:925–32.
MLA
Valdés Vázquez, Yirina, Nicole Viaene, Vivian Blok, et al. “Changes in the Pre-parasitic Developmental Stage of Globodera Rostochiensis in Response to Green Manures.” NEMATOLOGY 14 (2012): 925–932. Print.