Ghent University Academic Bibliography

@article{2085481,
  abstract     = {Molecular mimetics of the caspase activator second mitochondria-derived activator of caspase (SMAC) are being investigated for use in cancer therapy, but an understanding of in vivo effects remains incomplete. In this study, we offer evidence that SMAC mimetics elicit a proinflammatory cell death in cancer cells that engages an adaptive antitumor immune response. Cancer cells of different histologic origin underwent apoptosis when transduced with lentiviral vectors encoding a cytosolic form of the SMAC mimetic LV-tSMAC. Strikingly, treatment of tumor-bearing mice with LV-tSMAC resulted in the induction of apoptosis, activation of antitumor immunity, and enhanced survival. Antitumor immunity was accompanied by an increase of tumor-infiltrating lymphocytes displaying low PD-1 expression, high Lytic capacity, and high levels of IFN-gamma when stimulated. We also noted in vivo a decrease in regulatory T cells along with in vitro activation of tumor-specific CD8(+) T cells by dendritic cells (DC) isolated from tumor draining lymph nodes. Last, tumor-specific cytotoxic T cells were also found to be activated in vivo. Mechanistic analyses showed that transduction of cancer cells with LV-tSMAC resulted in exposure of calreticulin but not release of HMGB1 or ATP. Nevertheless, DCs were activated upon engulfment of dying cancer cells. Further validation of these findings was obtained by their extension in a model of human melanoma using transcriptionally targeted LV-tSMAC. Together, our findings suggest that SMAC mimetics can elicit a proinflammatory cell death that is sufficient to activate adaptive antitumor immune responses in cancer.},
  author       = {Emeagi, Perpetua U and Van Lint, Sandra and Goyvaerts, Cleo and Maenhout, Sarah and Cauwels, Anje and McNeish, Lain A and Bos, Tomas and Heirman, Carlo and Thielennans, Kris and Aerts, Joeri L and Breckpot, Karine},
  issn         = {0008-5472},
  journal      = {CANCER RESEARCH},
  keyword      = {REGULATORY T-CELLS,OVARIAN-CARCINOMA CELLS,DENDRITIC CELLS,EXPRESSION,APOPTOSIS,PRO-SMAC,TGF-BETA,TUMOR-CELLS,CANCER-CELLS,LENTIVIRAL VECTORS},
  language     = {eng},
  number       = {6},
  pages        = {1342--1352},
  title        = {Proinflammatory characteristics of SMAC/DIABLO-induced cell death in antitumor therapy},
  url          = {http://dx.doi.org/10.1158/0008-5472.CAN-11-2400},
  volume       = {72},
  year         = {2012},
}

Chicago

Emeagi, Perpetua U, Sandra Van Lint, Cleo Goyvaerts, Sarah Maenhout, Anje Cauwels, Lain A McNeish, Tomas Bos, et al. 2012. “Proinflammatory Characteristics of SMAC/DIABLO-induced Cell Death in Antitumor Therapy.” Cancer Research 72 (6): 1342–1352.

APA

Emeagi, P. U., Van Lint, S., Goyvaerts, C., Maenhout, S., Cauwels, A., McNeish, L. A., Bos, T., et al. (2012). Proinflammatory characteristics of SMAC/DIABLO-induced cell death in antitumor therapy. CANCER RESEARCH, 72(6), 1342–1352.

Vancouver

1.

Emeagi PU, Van Lint S, Goyvaerts C, Maenhout S, Cauwels A, McNeish LA, et al. Proinflammatory characteristics of SMAC/DIABLO-induced cell death in antitumor therapy. CANCER RESEARCH. 2012;72(6):1342–52.

MLA

Emeagi, Perpetua U, Sandra Van Lint, Cleo Goyvaerts, et al. “Proinflammatory Characteristics of SMAC/DIABLO-induced Cell Death in Antitumor Therapy.” CANCER RESEARCH 72.6 (2012): 1342–1352. Print.