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Influence of the uterine environment on the development of in vitro-produced equine embryos

Katrien Smits UGent, Jan Govaere UGent, Luc Peelman UGent, Karen Goossens UGent, Dirk de Graaf UGent, Dries Vercauteren UGent, Leen Vandaele UGent, Maarten Hoogewijs UGent, Eline Wydooghe UGent and Torn Stout, et al. (2012) REPRODUCTION. 143(2). p.173-181
abstract
The necessity for early interaction between the embryo and the oviductal and/or uterine environment in the horse is reflected by several striking differences between equine embryos that develop in vivo and those produced in vitro. Better understanding of the salient interactions may help to improve the efficiency of in vitro equine embryo production. In an initial experiment, cleavage-stage in vitro-produced (IVP) equine embryos were transferred into the uterus of recipient mares that had ovulated recently to determine whether premature placement in this in vivo environment would improve subsequent development. In a second experiment, an important element of the uterine environment was mimicked by adding uterocalin, a major component of the endometrial secretions during early pregnancy, to the culture medium. Intrauterine transfer of cleavage-stage IVP equine embryos yielded neither ultrasonographically detectable pregnancies nor day 7 blastocysts, indicating that the uterus is not a suitable environment for pre-compact morula stage horse embryos. By contrast, exposure to uterocalin during IVP improved capsule formation, although it did not measurably affect the development or expression of a panel of genes known to differ between in vivo and in vitro embryos. Further studies are required to evaluate whether uterocalin serves purely as a carrier protein or more directly promotes improved capsule development.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DAY-4 EMBRYOS, BLASTOCYST CAPSULE, MARE, VIVO, CONCEPTUS, PROTEIN, UTERUS, UTEROCALIN, MORPHOLOGY, PREGNANCY
journal title
REPRODUCTION
Reproduction
volume
143
issue
2
pages
173 - 181
Web of Science type
Article
Web of Science id
000301396500004
JCR category
REPRODUCTIVE BIOLOGY
JCR impact factor
3.555 (2012)
JCR rank
6/28 (2012)
JCR quartile
1 (2012)
ISSN
1470-1626
DOI
10.1530/REP-11-0217
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2082117
handle
http://hdl.handle.net/1854/LU-2082117
date created
2012-04-06 14:53:04
date last changed
2012-04-11 15:27:58
@article{2082117,
  abstract     = {The necessity for early interaction between the embryo and the oviductal and/or uterine environment in the horse is reflected by several striking differences between equine embryos that develop in vivo and those produced in vitro. Better understanding of the salient interactions may help to improve the efficiency of in vitro equine embryo production. In an initial experiment, cleavage-stage in vitro-produced (IVP) equine embryos were transferred into the uterus of recipient mares that had ovulated recently to determine whether premature placement in this in vivo environment would improve subsequent development. In a second experiment, an important element of the uterine environment was mimicked by adding uterocalin, a major component of the endometrial secretions during early pregnancy, to the culture medium. Intrauterine transfer of cleavage-stage IVP equine embryos yielded neither ultrasonographically detectable pregnancies nor day 7 blastocysts, indicating that the uterus is not a suitable environment for pre-compact morula stage horse embryos. By contrast, exposure to uterocalin during IVP improved capsule formation, although it did not measurably affect the development or expression of a panel of genes known to differ between in vivo and in vitro embryos. Further studies are required to evaluate whether uterocalin serves purely as a carrier protein or more directly promotes improved capsule development.},
  author       = {Smits, Katrien and Govaere, Jan and Peelman, Luc and Goossens, Karen and de Graaf, Dirk and Vercauteren, Dries and Vandaele, Leen and Hoogewijs, Maarten and Wydooghe, Eline and Stout, Torn and Van Soom, Ann},
  issn         = {1470-1626},
  journal      = {REPRODUCTION},
  keyword      = {DAY-4 EMBRYOS,BLASTOCYST CAPSULE,MARE,VIVO,CONCEPTUS,PROTEIN,UTERUS,UTEROCALIN,MORPHOLOGY,PREGNANCY},
  language     = {eng},
  number       = {2},
  pages        = {173--181},
  title        = {Influence of the uterine environment on the development of in vitro-produced equine embryos},
  url          = {http://dx.doi.org/10.1530/REP-11-0217},
  volume       = {143},
  year         = {2012},
}

Chicago
Smits, Katrien, Jan Govaere, Luc Peelman, Karen Goossens, Dirk de Graaf, Dries Vercauteren, Leen Vandaele, et al. 2012. “Influence of the Uterine Environment on the Development of in Vitro-produced Equine Embryos.” Reproduction 143 (2): 173–181.
APA
Smits, Katrien, Govaere, J., Peelman, L., Goossens, K., de Graaf, D., Vercauteren, D., Vandaele, L., et al. (2012). Influence of the uterine environment on the development of in vitro-produced equine embryos. REPRODUCTION, 143(2), 173–181.
Vancouver
1.
Smits K, Govaere J, Peelman L, Goossens K, de Graaf D, Vercauteren D, et al. Influence of the uterine environment on the development of in vitro-produced equine embryos. REPRODUCTION. 2012;143(2):173–81.
MLA
Smits, Katrien, Jan Govaere, Luc Peelman, et al. “Influence of the Uterine Environment on the Development of in Vitro-produced Equine Embryos.” REPRODUCTION 143.2 (2012): 173–181. Print.