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Influence of the uterine environment on the development of in vitro-produced equine embryos

Katrien Smits (UGent) , Jan Govaere (UGent) , Luc Peelman (UGent) , Karen Goossens, Dirk de Graaf (UGent) , Dries Vercauteren (UGent) , Leen Vandaele (UGent) , Maarten Hoogewijs (UGent) , Eline Wydooghe (UGent) , Torn Stout, et al.
(2012) REPRODUCTION. 143(2). p.173-181
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Abstract
The necessity for early interaction between the embryo and the oviductal and/or uterine environment in the horse is reflected by several striking differences between equine embryos that develop in vivo and those produced in vitro. Better understanding of the salient interactions may help to improve the efficiency of in vitro equine embryo production. In an initial experiment, cleavage-stage in vitro-produced (IVP) equine embryos were transferred into the uterus of recipient mares that had ovulated recently to determine whether premature placement in this in vivo environment would improve subsequent development. In a second experiment, an important element of the uterine environment was mimicked by adding uterocalin, a major component of the endometrial secretions during early pregnancy, to the culture medium. Intrauterine transfer of cleavage-stage IVP equine embryos yielded neither ultrasonographically detectable pregnancies nor day 7 blastocysts, indicating that the uterus is not a suitable environment for pre-compact morula stage horse embryos. By contrast, exposure to uterocalin during IVP improved capsule formation, although it did not measurably affect the development or expression of a panel of genes known to differ between in vivo and in vitro embryos. Further studies are required to evaluate whether uterocalin serves purely as a carrier protein or more directly promotes improved capsule development.
Keywords
DAY-4 EMBRYOS, BLASTOCYST CAPSULE, MARE, VIVO, CONCEPTUS, PROTEIN, UTERUS, UTEROCALIN, MORPHOLOGY, PREGNANCY

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Chicago
Smits, Katrien, Jan Govaere, Luc Peelman, Karen Goossens, Dirk de Graaf, Dries Vercauteren, Leen Vandaele, et al. 2012. “Influence of the Uterine Environment on the Development of in Vitro-produced Equine Embryos.” Reproduction 143 (2): 173–181.
APA
Smits, Katrien, Govaere, J., Peelman, L., Goossens, K., de Graaf, D., Vercauteren, D., Vandaele, L., et al. (2012). Influence of the uterine environment on the development of in vitro-produced equine embryos. REPRODUCTION, 143(2), 173–181.
Vancouver
1.
Smits K, Govaere J, Peelman L, Goossens K, de Graaf D, Vercauteren D, et al. Influence of the uterine environment on the development of in vitro-produced equine embryos. REPRODUCTION. 2012;143(2):173–81.
MLA
Smits, Katrien, Jan Govaere, Luc Peelman, et al. “Influence of the Uterine Environment on the Development of in Vitro-produced Equine Embryos.” REPRODUCTION 143.2 (2012): 173–181. Print.
@article{2082117,
  abstract     = {The necessity for early interaction between the embryo and the oviductal and/or uterine environment in the horse is reflected by several striking differences between equine embryos that develop in vivo and those produced in vitro. Better understanding of the salient interactions may help to improve the efficiency of in vitro equine embryo production. In an initial experiment, cleavage-stage in vitro-produced (IVP) equine embryos were transferred into the uterus of recipient mares that had ovulated recently to determine whether premature placement in this in vivo environment would improve subsequent development. In a second experiment, an important element of the uterine environment was mimicked by adding uterocalin, a major component of the endometrial secretions during early pregnancy, to the culture medium. Intrauterine transfer of cleavage-stage IVP equine embryos yielded neither ultrasonographically detectable pregnancies nor day 7 blastocysts, indicating that the uterus is not a suitable environment for pre-compact morula stage horse embryos. By contrast, exposure to uterocalin during IVP improved capsule formation, although it did not measurably affect the development or expression of a panel of genes known to differ between in vivo and in vitro embryos. Further studies are required to evaluate whether uterocalin serves purely as a carrier protein or more directly promotes improved capsule development.},
  author       = {Smits, Katrien and Govaere, Jan and Peelman, Luc and Goossens, Karen and de Graaf, Dirk and Vercauteren, Dries and Vandaele, Leen and Hoogewijs, Maarten and Wydooghe, Eline and Stout, Torn and Van Soom, Ann},
  issn         = {1470-1626},
  journal      = {REPRODUCTION},
  language     = {eng},
  number       = {2},
  pages        = {173--181},
  title        = {Influence of the uterine environment on the development of in vitro-produced equine embryos},
  url          = {http://dx.doi.org/10.1530/REP-11-0217},
  volume       = {143},
  year         = {2012},
}

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