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Successful hydraulic strategies to start up OLAND sequencing batch reactors at lab scale

Thomas Schaubroeck UGent, Samik Bagchi, Haydée De Clippeleir UGent, Marta Carballa, Willy Verstraete UGent and Siegfried Vlaeminck UGent (2012) MICROBIAL BIOTECHNOLOGY. 5(3). p.403-414
abstract
Oxygen-limited autotrophic nitrification/denitrification (OLAND) is a one-stage combination of partial nitritation and anammox, which can have a challenging process start-up. In this study, start-up strategies were tested for sequencing batch reactors (SBR), varying hydraulic parameters, i.e. volumetric exchange ratio (VER) and feeding regime, and salinity. Two sequential tests with two parallel SBR were performed, and stable removal rates > 0.4 g N l-1 day-1 with minimal nitrite and nitrate accumulation were considered a successful start-up. SBR A and B were operated at 50% VER with 3 g NaCl l-1 in the influent, and the influent was fed over 8% and 82% of the cycle time respectively. SBR B started up in 24 days, but SBR A achieved no start-up in 39 days. SBR C and D were fed over 65% of the cycle time at 25% VER, and salt was added only to the influent of SBR D (5 g NaCl l-1). Start-up of both SBR C and D was successful in 9 and 32 days respectively. Reactor D developed a higher proportion of small aggregates (0.100.25 mm), with a high nitritation to anammox rate ratio, likely the cause of the observed nitrite accumulation. The latter was overcome by temporarily including an anoxic period at the end of the reaction phase. All systems achieved granulation and similar biomass-specific nitrogen removal rates (141220 mg N g-1 VSS day-1). FISH revealed a close juxtapositioning of aerobic and anoxic ammonium-oxidizing bacteria (AerAOB and AnAOB), also in small aggregates. DGGE showed that AerAOB communities had a lower evenness than Planctomycetes communities. A higher richness of the latter seemed to be correlated with better reactor performance. Overall, the fast start-up of SBR B, C and D suggests that stable hydraulic conditions are beneficial for OLAND while increased salinity at the tested levels is not needed for good reactor performance.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ANAMMOX PROCESS, DEAMMONIFICATION, GRANULAR SLUDGE, WASTE-WATER, NITROGEN-REMOVAL, ROTATING BIOLOGICAL CONTACTOR, STAGE PARTIAL NITRITATION, NITRIFICATION, BIOFILM, SYSTEM
journal title
MICROBIAL BIOTECHNOLOGY
Microb. Biotechnol.
volume
5
issue
3
pages
403 - 414
Web of Science type
Article
Web of Science id
000302858900010
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.214 (2012)
JCR rank
41/157 (2012)
JCR quartile
2 (2012)
ISSN
1751-7907
DOI
10.1111/j.1751-7915.2011.00326.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2081654
handle
http://hdl.handle.net/1854/LU-2081654
date created
2012-04-05 17:21:34
date last changed
2013-01-16 14:38:05
@article{2081654,
  abstract     = {Oxygen-limited autotrophic nitrification/denitrification (OLAND) is a one-stage combination of partial nitritation and anammox, which can have a challenging process start-up. In this study, start-up strategies were tested for sequencing batch reactors (SBR), varying hydraulic parameters, i.e. volumetric exchange ratio (VER) and feeding regime, and salinity. Two sequential tests with two parallel SBR were performed, and stable removal rates {\textrangle} 0.4 g N l-1 day-1 with minimal nitrite and nitrate accumulation were considered a successful start-up. SBR A and B were operated at 50\% VER with 3 g NaCl l-1 in the influent, and the influent was fed over 8\% and 82\% of the cycle time respectively. SBR B started up in 24 days, but SBR A achieved no start-up in 39 days. SBR C and D were fed over 65\% of the cycle time at 25\% VER, and salt was added only to the influent of SBR D (5 g NaCl l-1). Start-up of both SBR C and D was successful in 9 and 32 days respectively. Reactor D developed a higher proportion of small aggregates (0.100.25 mm), with a high nitritation to anammox rate ratio, likely the cause of the observed nitrite accumulation. The latter was overcome by temporarily including an anoxic period at the end of the reaction phase. All systems achieved granulation and similar biomass-specific nitrogen removal rates (141220 mg N g-1 VSS day-1). FISH revealed a close juxtapositioning of aerobic and anoxic ammonium-oxidizing bacteria (AerAOB and AnAOB), also in small aggregates. DGGE showed that AerAOB communities had a lower evenness than Planctomycetes communities. A higher richness of the latter seemed to be correlated with better reactor performance. Overall, the fast start-up of SBR B, C and D suggests that stable hydraulic conditions are beneficial for OLAND while increased salinity at the tested levels is not needed for good reactor performance.},
  author       = {Schaubroeck, Thomas and Bagchi, Samik and De Clippeleir, Hayd{\'e}e and Carballa, Marta and Verstraete, Willy and Vlaeminck, Siegfried},
  issn         = {1751-7907},
  journal      = {MICROBIAL BIOTECHNOLOGY},
  keyword      = {ANAMMOX PROCESS,DEAMMONIFICATION,GRANULAR SLUDGE,WASTE-WATER,NITROGEN-REMOVAL,ROTATING BIOLOGICAL CONTACTOR,STAGE PARTIAL NITRITATION,NITRIFICATION,BIOFILM,SYSTEM},
  language     = {eng},
  number       = {3},
  pages        = {403--414},
  title        = {Successful hydraulic strategies to start up OLAND sequencing batch reactors at lab scale},
  url          = {http://dx.doi.org/10.1111/j.1751-7915.2011.00326.x},
  volume       = {5},
  year         = {2012},
}

Chicago
Schaubroeck, Thomas, Samik Bagchi, Haydée De Clippeleir, Marta Carballa, Willy Verstraete, and Siegfried Vlaeminck. 2012. “Successful Hydraulic Strategies to Start up OLAND Sequencing Batch Reactors at Lab Scale.” Microbial Biotechnology 5 (3): 403–414.
APA
Schaubroeck, T., Bagchi, S., De Clippeleir, H., Carballa, M., Verstraete, W., & Vlaeminck, S. (2012). Successful hydraulic strategies to start up OLAND sequencing batch reactors at lab scale. MICROBIAL BIOTECHNOLOGY, 5(3), 403–414.
Vancouver
1.
Schaubroeck T, Bagchi S, De Clippeleir H, Carballa M, Verstraete W, Vlaeminck S. Successful hydraulic strategies to start up OLAND sequencing batch reactors at lab scale. MICROBIAL BIOTECHNOLOGY. 2012;5(3):403–14.
MLA
Schaubroeck, Thomas, Samik Bagchi, Haydée De Clippeleir, et al. “Successful Hydraulic Strategies to Start up OLAND Sequencing Batch Reactors at Lab Scale.” MICROBIAL BIOTECHNOLOGY 5.3 (2012): 403–414. Print.