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An Arabidopsis thaliana pectin acetylesterase gene is upregulated in nematode feeding sites induced by root-knot and cyst nematodes

Isabel Vercauteren, Janice de Almeida Engler UGent, Ruth De Groodt and Godelieve Gheysen UGent (2002) MOLECULAR PLANT-MICROBE INTERACTIONS. 15(4). p.404-407
abstract
By using differential display, gene expression was investigated in Arabidopsis thaliana roots shortly after nematode infection, and a putative pectin acetylesterase (PAE) homolog (DiDi 9C-12) was found to be upregulated. PAEs catalyze the deacetylation of pectin, a major compound of primary cell walls. mRNA in situ hybridization experiments showed that the expression of DiDi 9C-12 was enhanced very early after infection in initiating giant-cells and in cells surrounding the nematodes. Later on, the level of DiDi 9C-12 mRNA was lower in giant-cells and transcripts were mainly found in parenchyma, endodermis, and pericycle cells of the root gall. Twenty days after infection, DiDi 9C-12 transcripts could no longer be detected. DiDi 9C-12 transcripts were also found in young syncytia and in the cells surrounding the expanding syncytium. Our results suggest that plant parasitic nematodes can modulate the rapid growth of the feeding cells and the expansion of the root gall by triggering the expression of DiDi 9C-12. PAEs, which probably act together with a range of other pectin-degrading enzymes, could be involved in softening and loosening the primary cell wall in nematode-infected plant roots.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Meloidogyne incognita, Heterodera schachtii
journal title
MOLECULAR PLANT-MICROBE INTERACTIONS
Mol. Plant-Microbe Interact.
volume
15
issue
4
pages
404 - 407
Web of Science type
Article
Web of Science id
000174820200013
JCR category
PLANT SCIENCES
JCR impact factor
3.845 (2002)
JCR rank
9/134 (2002)
JCR quartile
1 (2002)
ISSN
0894-0282
DOI
10.1094/MPMI.2002.15.4.404
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
208123
handle
http://hdl.handle.net/1854/LU-208123
date created
2008-01-11 10:41:00
date last changed
2016-12-19 15:38:03
@article{208123,
  abstract     = {By using differential display, gene expression was investigated in Arabidopsis thaliana roots shortly after nematode infection, and a putative pectin acetylesterase (PAE) homolog (DiDi 9C-12) was found to be upregulated. PAEs catalyze the deacetylation of pectin, a major compound of primary cell walls. mRNA in situ hybridization experiments showed that the expression of DiDi 9C-12 was enhanced very early after infection in initiating giant-cells and in cells surrounding the nematodes. Later on, the level of DiDi 9C-12 mRNA was lower in giant-cells and transcripts were mainly found in parenchyma, endodermis, and pericycle cells of the root gall. Twenty days after infection, DiDi 9C-12 transcripts could no longer be detected. DiDi 9C-12 transcripts were also found in young syncytia and in the cells surrounding the expanding syncytium. Our results suggest that plant parasitic nematodes can modulate the rapid growth of the feeding cells and the expansion of the root gall by triggering the expression of DiDi 9C-12. PAEs, which probably act together with a range of other pectin-degrading enzymes, could be involved in softening and loosening the primary cell wall in nematode-infected plant roots.},
  author       = {Vercauteren, Isabel and de Almeida Engler, Janice and De Groodt, Ruth and Gheysen, Godelieve},
  issn         = {0894-0282},
  journal      = {MOLECULAR PLANT-MICROBE INTERACTIONS},
  keyword      = {Meloidogyne incognita,Heterodera schachtii},
  language     = {eng},
  number       = {4},
  pages        = {404--407},
  title        = {An Arabidopsis thaliana pectin acetylesterase gene is upregulated in nematode feeding sites induced by root-knot and cyst nematodes},
  url          = {http://dx.doi.org/10.1094/MPMI.2002.15.4.404},
  volume       = {15},
  year         = {2002},
}

Chicago
Vercauteren, Isabel, Janice de Almeida Engler, Ruth De Groodt, and Godelieve Gheysen. 2002. “An Arabidopsis Thaliana Pectin Acetylesterase Gene Is Upregulated in Nematode Feeding Sites Induced by Root-knot and Cyst Nematodes.” Molecular Plant-microbe Interactions 15 (4): 404–407.
APA
Vercauteren, Isabel, de Almeida Engler, J., De Groodt, R., & Gheysen, G. (2002). An Arabidopsis thaliana pectin acetylesterase gene is upregulated in nematode feeding sites induced by root-knot and cyst nematodes. MOLECULAR PLANT-MICROBE INTERACTIONS, 15(4), 404–407.
Vancouver
1.
Vercauteren I, de Almeida Engler J, De Groodt R, Gheysen G. An Arabidopsis thaliana pectin acetylesterase gene is upregulated in nematode feeding sites induced by root-knot and cyst nematodes. MOLECULAR PLANT-MICROBE INTERACTIONS. 2002;15(4):404–7.
MLA
Vercauteren, Isabel, Janice de Almeida Engler, Ruth De Groodt, et al. “An Arabidopsis Thaliana Pectin Acetylesterase Gene Is Upregulated in Nematode Feeding Sites Induced by Root-knot and Cyst Nematodes.” MOLECULAR PLANT-MICROBE INTERACTIONS 15.4 (2002): 404–407. Print.