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Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women

Mambo-Claude Muvunyi UGent, LAURENS CLAEYS UGent, Tineke De Sutter UGent, Petra De Sutter UGent, Marleen Temmerman UGent, Lieve Van Renterghem, Geert Claeys UGent and Elizaveta Padalko UGent (2012) JOURNAL OF INFECTION IN DEVELOPING COUNTRIES. 6(5). p.396-402
abstract
Introduction: Chlamydia antibody testing (CAT) in serum has been introduced as a screening method in the infertility workup. We evaluated the test characteristics of two ELISA tests compared to micro-immunofluorescence tests (MIFs). MIFs are considered the gold standard in the C. trachomatis IgG antibodies detection. We also compared the accuracy of all CAT tests in predicting tubal subfertility, using laparoscopy as a reference. Methodology: Four commercial serological methods were used to analyse 101 serum samples for the presence of C. trachomatis IgG antibodies from patients at the Infertility Clinic of Ghent University Hospital. The diagnostic utility for prediction of tubal infertility of serological methods was evaluated based on patients' medical records. Results: A comparison of the serological assays showed little difference in the major performance characteristics: the sensitivities of all MIFs and ELISAs were 100% for all assays (except the ELISA Vircell, with a sensitivity of 90%), and the specificities ranged from 92% for MIF Ani Labsystems to 98% for the MIF Focus and ELISA Vircell. As compared to laparoscopy data, CAT positivity in subfertile women with tubal damage (n=40) did not significantly differ from that of subfertile women without tubal damage (n=61): Positive predictive values (PPV) of CAT ranged from 53% to 60% and negative predictive values (NPV) ranged from 62% to 64%. Conclusion: evaluated ELISAs are comparable to MIFs in the detection of C. trachomatis IgG antibodies and should be preferred for large serological studies, especially in resource poor settings.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Chlamydia trachomatis, antibody, serology, subfertility, TUBAL FACTOR SUBFERTILITY, MICROIMMUNOFLUORESCENCE ASSAY, IMMUNOGLOBULIN-G, ANTIBODY-TITERS, INFERTILE WOMEN, TESTS, INFECTION, DAMAGE, IGG, METAANALYSIS
journal title
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
J. Infect. Dev. Ctries.
volume
6
issue
5
pages
396 - 402
Web of Science type
Article
Web of Science id
000305911500004
JCR category
INFECTIOUS DISEASES
JCR impact factor
0.996 (2012)
JCR rank
63/69 (2012)
JCR quartile
4 (2012)
ISSN
1972-2680
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2076832
handle
http://hdl.handle.net/1854/LU-2076832
date created
2012-03-29 12:37:54
date last changed
2012-09-20 10:58:45
@article{2076832,
  abstract     = {Introduction: Chlamydia antibody testing (CAT) in serum has been introduced as a screening method in the infertility workup. We evaluated the test characteristics of two ELISA tests compared to micro-immunofluorescence tests (MIFs). MIFs are considered the gold standard in the C. trachomatis IgG antibodies detection. We also compared the accuracy of all CAT tests in predicting tubal subfertility, using laparoscopy as a reference. 
Methodology: Four commercial serological methods were used to analyse 101 serum samples for the presence of C. trachomatis IgG antibodies from patients at the Infertility Clinic of Ghent University Hospital. The diagnostic utility for prediction of tubal infertility of serological methods was evaluated based on patients' medical records. 
Results: A comparison of the serological assays showed little difference in the major performance characteristics: the sensitivities of all MIFs and ELISAs were 100\% for all assays (except the ELISA Vircell, with a sensitivity of 90\%), and the specificities ranged from 92\% for MIF Ani Labsystems to 98\% for the MIF Focus and ELISA Vircell. As compared to laparoscopy data, CAT positivity in subfertile women with tubal damage (n=40) did not significantly differ from that of subfertile women without tubal damage (n=61): Positive predictive values (PPV) of CAT ranged from 53\% to 60\% and negative predictive values (NPV) ranged from 62\% to 64\%. 
Conclusion: evaluated ELISAs are comparable to MIFs in the detection of C. trachomatis IgG antibodies and should be preferred for large serological studies, especially in resource poor settings.},
  author       = {Muvunyi, Mambo-Claude and CLAEYS, LAURENS and De Sutter, Tineke and De Sutter, Petra and Temmerman, Marleen and Van Renterghem, Lieve and Claeys, Geert and Padalko, Elizaveta},
  issn         = {1972-2680},
  journal      = {JOURNAL OF INFECTION IN DEVELOPING COUNTRIES},
  keyword      = {Chlamydia trachomatis,antibody,serology,subfertility,TUBAL FACTOR SUBFERTILITY,MICROIMMUNOFLUORESCENCE ASSAY,IMMUNOGLOBULIN-G,ANTIBODY-TITERS,INFERTILE WOMEN,TESTS,INFECTION,DAMAGE,IGG,METAANALYSIS},
  language     = {eng},
  number       = {5},
  pages        = {396--402},
  title        = {Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women},
  volume       = {6},
  year         = {2012},
}

Chicago
Muvunyi, Mambo-Claude, LAURENS CLAEYS, Tineke De Sutter, Petra De Sutter, Marleen Temmerman, Lieve Van Renterghem, Geert Claeys, and ELIZAVETA PADALKO. 2012. “Comparison of Four Serological Assays for the Diagnosis of Chlamydia Trachomatis in Subfertile Women.” Journal of Infection in Developing Countries 6 (5): 396–402.
APA
Muvunyi, M.-C., CLAEYS, L., De Sutter, T., De Sutter, P., Temmerman, M., Van Renterghem, L., Claeys, G., et al. (2012). Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women. JOURNAL OF INFECTION IN DEVELOPING COUNTRIES, 6(5), 396–402.
Vancouver
1.
Muvunyi M-C, CLAEYS L, De Sutter T, De Sutter P, Temmerman M, Van Renterghem L, et al. Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women. JOURNAL OF INFECTION IN DEVELOPING COUNTRIES. 2012;6(5):396–402.
MLA
Muvunyi, Mambo-Claude, LAURENS CLAEYS, Tineke De Sutter, et al. “Comparison of Four Serological Assays for the Diagnosis of Chlamydia Trachomatis in Subfertile Women.” JOURNAL OF INFECTION IN DEVELOPING COUNTRIES 6.5 (2012): 396–402. Print.