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Predicting drug penetration across the blood-brain barrier: comparison of micellar liquid chromatography and immobilized artificial membrane liquid chromatography

Mike De Vrieze (UGent) , Frederic Lynen (UGent) and Patrick Sandra (UGent)
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Abstract
Several in vitro methods have been investigated for mimicking drug penetration across the blood-brain barrier (BBB) into the central nervous system (CNS). Both micellar liquid chromatography (MLC) and immobilized artificial membrane (IAM) liquid chromatography were tested in this contribution in order to construct models for BBB transfer prediction. MLC measurements were performed on a C18-column with sodium dodecyl sulfate (SDS), polyoxyethylene (23) lauryl ether (Brij35) or sodium deoxycholate (SDC) as surfactants in the micellar mobile phase. IAM liquid chromatography measurements were performed with a Dulbecco’s phosphate-buffered saline (DPBS) and a certain percentage of methanol as organic modifier in the mobile phase. This study aimed to obtain a high correlation between in vivo and predicted log BB values (= concentration of the drug molecule in the brain to concentration in the blood).
Keywords
MLC, BBB, IAM

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Chicago
De Vrieze, Mike, Frederic Lynen, and Patrick Sandra. 2012. “Predicting Drug Penetration Across the Blood-brain Barrier: Comparison of Micellar Liquid Chromatography and Immobilized Artificial Membrane Liquid Chromatography.” In 12th International Symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers (HTC-12) and 2nd International Symposium on Hyphenated Techniques for Sample Preparation (HTSP-2), Book of Abstracts, ed. Etienne Jooken. Koninklijke Vlaamse Chemische Vereniging (KVCV).
APA
De Vrieze, M., Lynen, F., & Sandra, P. (2012). Predicting drug penetration across the blood-brain barrier: comparison of micellar liquid chromatography and immobilized artificial membrane liquid chromatography. In E. Jooken (Ed.), 12th International symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers (HTC-12) and 2nd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-2), Book of abstracts. Presented at the 12th International symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers (HTC-12) ; 2nd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-2), Koninklijke Vlaamse Chemische Vereniging (KVCV).
Vancouver
1.
De Vrieze M, Lynen F, Sandra P. Predicting drug penetration across the blood-brain barrier: comparison of micellar liquid chromatography and immobilized artificial membrane liquid chromatography. In: Jooken E, editor. 12th International symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers (HTC-12) and 2nd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-2), Book of abstracts. Koninklijke Vlaamse Chemische Vereniging (KVCV); 2012.
MLA
De Vrieze, Mike, Frederic Lynen, and Patrick Sandra. “Predicting Drug Penetration Across the Blood-brain Barrier: Comparison of Micellar Liquid Chromatography and Immobilized Artificial Membrane Liquid Chromatography.” 12th International Symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers (HTC-12) and 2nd International Symposium on Hyphenated Techniques for Sample Preparation (HTSP-2), Book of Abstracts. Ed. Etienne Jooken. Koninklijke Vlaamse Chemische Vereniging (KVCV), 2012. Print.
@inproceedings{2076726,
  abstract     = {Several in vitro methods have been investigated for mimicking drug penetration across the blood-brain barrier (BBB) into the central nervous system (CNS). Both micellar liquid chromatography (MLC) and immobilized artificial membrane (IAM) liquid chromatography were tested in this contribution in order to construct models for BBB transfer prediction. MLC measurements were performed on a C18-column with sodium dodecyl sulfate (SDS), polyoxyethylene (23) lauryl ether (Brij35) or sodium deoxycholate (SDC) as surfactants in the micellar mobile phase. IAM liquid chromatography measurements were performed with a Dulbecco{\textquoteright}s phosphate-buffered saline (DPBS) and a certain percentage of methanol as organic modifier in the mobile phase. This study aimed to obtain a high correlation between in vivo and predicted log BB values (= concentration of the drug molecule in the brain to concentration in the blood).},
  author       = {De Vrieze, Mike and Lynen, Frederic and Sandra, Patrick},
  booktitle    = {12th International symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers (HTC-12) and 2nd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-2), Book of abstracts},
  editor       = {Jooken, Etienne},
  isbn         = {9789081124843},
  keyword      = {MLC,BBB,IAM},
  language     = {eng},
  location     = {Brugge, Belgium},
  publisher    = {Koninklijke Vlaamse Chemische Vereniging (KVCV)},
  title        = {Predicting drug penetration across the blood-brain barrier: comparison of micellar liquid chromatography and immobilized artificial membrane liquid chromatography},
  year         = {2012},
}