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Influence of vasoactive intestinal polypeptide and NG-nitro-L-arginine methyl ester on cholinergic neurotransmission in the rat gastric fundus

(1992) EUROPEAN JOURNAL OF PHARMACOLOGY. 221(2-3). p.235-242
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Abstract
The possible modulating effect of vasoactive intestinal polypeptide (VIP) and nitric oxide (NO), on cholinergic neurotransmission was assessed in longitudinal muscle strips of rat gastric fundus. VIP and NO are the putative co-transmitters of the inhibitory non-adrenergic non-cholinergic (NANC) neurones in this tissue. VIP concentration dependently inhibited cholinergic contractions induced by 2-min transmural stimulation, relaxed_tissues, the tone of which was continuously raised by transmural stimulation, and shifted to the right the frequency-response curves for contraction induced by transmural stimulation with a cumulative increase of frequency. The same effect was found when contractions were induced with methacholine, suggesting that only functional antagonism at the postsynaptic smooth muscle cell level is involved. On 30-min incubations, 3 x 10(-4) M N(G)-nitro-L-arginine methyl ester (L-NAME) potentiated cholinergic responses to 20-s transmural stimulation, while not influencing contractions of similar amplitude evoked by methacholine; the cholinergic responses to 2-min transmural stimulation were also not influenced. The potentiating effect of L-NAME was prevented by L-arginine but not D-arginine. These results suggest that endogenous NO released from the inhibitory NANC neurones during short trains of transmural stimulation interferes with cholinergic neurotransmission either by functional antagonism of acetylcholine at the postsynaptic level or by presynaptic inhibition of acetylcholine release.
Keywords
VIP, TRANSMISSION, VIP (VASOACTIVE INTESTINAL POLYPEPTIDE), GASTRIC FUNDUS (RAT), NITRIC OXIDE (NO), CHOLINERGIC NEUROTRANSMISSION, RESPIRATORY-TRACT, TRACHEAL SMOOTH-MUSCLE, OXIDE, MODULATION, PEPTIDE, STIMULATION, INVITRO, MOTILITY

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Chicago
Lefebvre, Romain, An De Vriese, and Geert JM Smits. 1992. “Influence of Vasoactive Intestinal Polypeptide and NG-nitro-L-arginine Methyl Ester on Cholinergic Neurotransmission in the Rat Gastric Fundus.” European Journal of Pharmacology 221 (2-3): 235–242.
APA
Lefebvre, R., De Vriese, A., & Smits, G. J. (1992). Influence of vasoactive intestinal polypeptide and NG-nitro-L-arginine methyl ester on cholinergic neurotransmission in the rat gastric fundus. EUROPEAN JOURNAL OF PHARMACOLOGY, 221(2-3), 235–242.
Vancouver
1.
Lefebvre R, De Vriese A, Smits GJ. Influence of vasoactive intestinal polypeptide and NG-nitro-L-arginine methyl ester on cholinergic neurotransmission in the rat gastric fundus. EUROPEAN JOURNAL OF PHARMACOLOGY. 1992;221(2-3):235–42.
MLA
Lefebvre, Romain, An De Vriese, and Geert JM Smits. “Influence of Vasoactive Intestinal Polypeptide and NG-nitro-L-arginine Methyl Ester on Cholinergic Neurotransmission in the Rat Gastric Fundus.” EUROPEAN JOURNAL OF PHARMACOLOGY 221.2-3 (1992): 235–242. Print.
@article{207092,
  abstract     = {The possible modulating effect of vasoactive intestinal polypeptide (VIP) and nitric oxide (NO), on cholinergic neurotransmission was assessed in longitudinal muscle strips of rat gastric fundus. VIP and NO are the putative co-transmitters of the inhibitory non-adrenergic non-cholinergic (NANC) neurones in this tissue. VIP concentration dependently inhibited cholinergic contractions induced by 2-min transmural stimulation, relaxed\_tissues, the tone of which was continuously raised by transmural stimulation, and shifted to the right the frequency-response curves for contraction induced by transmural stimulation with a cumulative increase of frequency. The same effect was found when contractions were induced with methacholine, suggesting that only functional antagonism at the postsynaptic smooth muscle cell level is involved. On 30-min incubations, 3 x 10(-4) M N(G)-nitro-L-arginine methyl ester (L-NAME) potentiated cholinergic responses to 20-s transmural stimulation, while not influencing contractions of similar amplitude evoked by methacholine; the cholinergic responses to 2-min transmural stimulation were also not influenced. The potentiating effect of L-NAME was prevented by L-arginine but not D-arginine. These results suggest that endogenous NO released from the inhibitory NANC neurones during short trains of transmural stimulation interferes with cholinergic neurotransmission either by functional antagonism of acetylcholine at the postsynaptic level or by presynaptic inhibition of acetylcholine release.},
  author       = {Lefebvre, Romain and De Vriese, An and Smits, Geert JM},
  issn         = {0014-2999},
  journal      = {EUROPEAN JOURNAL OF PHARMACOLOGY},
  keyword      = {VIP,TRANSMISSION,VIP (VASOACTIVE INTESTINAL POLYPEPTIDE),GASTRIC FUNDUS (RAT),NITRIC OXIDE (NO),CHOLINERGIC NEUROTRANSMISSION,RESPIRATORY-TRACT,TRACHEAL SMOOTH-MUSCLE,OXIDE,MODULATION,PEPTIDE,STIMULATION,INVITRO,MOTILITY},
  language     = {eng},
  number       = {2-3},
  pages        = {235--242},
  title        = {Influence of vasoactive intestinal polypeptide and NG-nitro-L-arginine methyl ester on cholinergic neurotransmission in the rat gastric fundus},
  url          = {http://dx.doi.org/10.1016/0014-2999(92)90707-B},
  volume       = {221},
  year         = {1992},
}

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