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Combined linkage and association mapping reveals CYCD5;1 as a quantitative trait gene for endoreduplication in Arabidopsis

Roel Sterken UGent, Raphaël Kiekens UGent, Joanna Boruc UGent, Fanghong Zhang UGent, Annelies Vercauteren UGent, Ilse Vercauteren UGent, Lien De Smet UGent, Stijn Dhondt UGent, Dirk Inzé UGent and Lieven De Veylder UGent, et al. (2012) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 109(12). p.4678-4683
abstract
Endoreduplication is the process where a cell replicates its genome without mitosis and cytokinesis, often followed by cell differentiation. This alternative cell cycle results in various levels of endoploidy, reaching 4x or higher one haploid set of chromosomes. Endoreduplication is found in animals and is widespread in plants, where it plays a major role in cellular differentiation and plant development. Here, we show that variation in endoreduplication between Arabidopsis thaliana accessions Columbia-0 and Kashmir is controlled by two major quantitative trait loci, ENDO-1 and ENDO-2. A local candidate gene association analysis in a set of 87 accessions, combined with expression analysis, identified CYCD5;1 as the most likely candidate gene underlying ENDO-2, operating as a rate-determining factor of endoreduplication. In accordance, both the overexpression and silencing of CYCD5;1 were effective in changing DNA ploidy levels, confirming CYCD5;1 to be a previously undescribed quantitative trait gene underlying endoreduplication in Arabidopsis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CYCLIN-DEPENDENT KINASE, regulatory haplotype, endopolyploidy, change-of-function allele, MAYS SSP PARVIGLUMIS, GENOME-WIDE ANALYSIS, CANDIDATE GENE, CELL-SIZE, PLANT DEVELOPMENT, LEAF DEVELOPMENT, ENDOCYCLE ONSET, ORGAN GROWTH, THALIANA
journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Proc. Natl. Acad. Sci. USA
volume
109
issue
12
pages
4678 - 4683
Web of Science type
Article
Web of Science id
000301712600066
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
9.737 (2012)
JCR rank
4/56 (2012)
JCR quartile
1 (2012)
ISSN
0027-8424
DOI
10.1073/pnas.1120811109
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2070843
handle
http://hdl.handle.net/1854/LU-2070843
date created
2012-03-21 10:19:41
date last changed
2014-05-26 10:02:02
@article{2070843,
  abstract     = {Endoreduplication is the process where a cell replicates its genome without mitosis and cytokinesis, often followed by cell differentiation. This alternative cell cycle results in various levels of endoploidy, reaching 4x or higher one haploid set of chromosomes. Endoreduplication is found in animals and is widespread in plants, where it plays a major role in cellular differentiation and plant development. Here, we show that variation in endoreduplication between Arabidopsis thaliana accessions Columbia-0 and Kashmir is controlled by two major quantitative trait loci, ENDO-1 and ENDO-2. A local candidate gene association analysis in a set of 87 accessions, combined with expression analysis, identified CYCD5;1 as the most likely candidate gene underlying ENDO-2, operating as a rate-determining factor of endoreduplication. In accordance, both the overexpression and silencing of CYCD5;1 were effective in changing DNA ploidy levels, confirming CYCD5;1 to be a previously undescribed quantitative trait gene underlying endoreduplication in Arabidopsis.},
  author       = {Sterken, Roel and Kiekens, Rapha{\"e}l and Boruc, Joanna and Zhang, Fanghong and Vercauteren, Annelies and Vercauteren, Ilse and De Smet, Lien and Dhondt, Stijn and Inz{\'e}, Dirk and De Veylder, Lieven and Russinova, Eugenia and Vuylsteke, Marnik},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {CYCLIN-DEPENDENT KINASE,regulatory haplotype,endopolyploidy,change-of-function allele,MAYS SSP PARVIGLUMIS,GENOME-WIDE ANALYSIS,CANDIDATE GENE,CELL-SIZE,PLANT DEVELOPMENT,LEAF DEVELOPMENT,ENDOCYCLE ONSET,ORGAN GROWTH,THALIANA},
  language     = {eng},
  number       = {12},
  pages        = {4678--4683},
  title        = {Combined linkage and association mapping reveals CYCD5;1 as a quantitative trait gene for endoreduplication in Arabidopsis},
  url          = {http://dx.doi.org/10.1073/pnas.1120811109},
  volume       = {109},
  year         = {2012},
}

Chicago
Sterken, Roel, Raphaël Kiekens, Joanna Boruc, Fanghong Zhang, Annelies Vercauteren, Ilse Vercauteren, Lien De Smet, et al. 2012. “Combined Linkage and Association Mapping Reveals CYCD5;1 as a Quantitative Trait Gene for Endoreduplication in Arabidopsis.” Proceedings of the National Academy of Sciences of the United States of America 109 (12): 4678–4683.
APA
Sterken, R., Kiekens, R., Boruc, J., Zhang, F., Vercauteren, A., Vercauteren, I., De Smet, L., et al. (2012). Combined linkage and association mapping reveals CYCD5;1 as a quantitative trait gene for endoreduplication in Arabidopsis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(12), 4678–4683.
Vancouver
1.
Sterken R, Kiekens R, Boruc J, Zhang F, Vercauteren A, Vercauteren I, et al. Combined linkage and association mapping reveals CYCD5;1 as a quantitative trait gene for endoreduplication in Arabidopsis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2012;109(12):4678–83.
MLA
Sterken, Roel, Raphaël Kiekens, Joanna Boruc, et al. “Combined Linkage and Association Mapping Reveals CYCD5;1 as a Quantitative Trait Gene for Endoreduplication in Arabidopsis.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109.12 (2012): 4678–4683. Print.