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Combined linkage and association mapping reveals CYCD5;1 as a quantitative trait gene for endoreduplication in Arabidopsis

Roel Sterken (UGent) , Raphaël Kiekens (UGent) , Joanna Boruc (UGent) , Fanghong Zhang (UGent) , Annelies Vercauteren (UGent) , Ilse Vercauteren (UGent) , Lien De Smet (UGent) , Stijn Dhondt (UGent) , Dirk Inzé (UGent) , Lieven De Veylder (UGent) , et al.
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Endoreduplication is the process where a cell replicates its genome without mitosis and cytokinesis, often followed by cell differentiation. This alternative cell cycle results in various levels of endoploidy, reaching 4x or higher one haploid set of chromosomes. Endoreduplication is found in animals and is widespread in plants, where it plays a major role in cellular differentiation and plant development. Here, we show that variation in endoreduplication between Arabidopsis thaliana accessions Columbia-0 and Kashmir is controlled by two major quantitative trait loci, ENDO-1 and ENDO-2. A local candidate gene association analysis in a set of 87 accessions, combined with expression analysis, identified CYCD5;1 as the most likely candidate gene underlying ENDO-2, operating as a rate-determining factor of endoreduplication. In accordance, both the overexpression and silencing of CYCD5;1 were effective in changing DNA ploidy levels, confirming CYCD5;1 to be a previously undescribed quantitative trait gene underlying endoreduplication in Arabidopsis.
Keywords
CYCLIN-DEPENDENT KINASE, regulatory haplotype, endopolyploidy, change-of-function allele, MAYS SSP PARVIGLUMIS, GENOME-WIDE ANALYSIS, CANDIDATE GENE, CELL-SIZE, PLANT DEVELOPMENT, LEAF DEVELOPMENT, ENDOCYCLE ONSET, ORGAN GROWTH, THALIANA

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Citation

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Chicago
Sterken, Roel, Raphaël Kiekens, Joanna Boruc, Fanghong Zhang, Annelies Vercauteren, Ilse Vercauteren, Lien De Smet, et al. 2012. “Combined Linkage and Association Mapping Reveals CYCD5;1 as a Quantitative Trait Gene for Endoreduplication in Arabidopsis.” Proceedings of the National Academy of Sciences of the United States of America 109 (12): 4678–4683.
APA
Sterken, R., Kiekens, R., Boruc, J., Zhang, F., Vercauteren, A., Vercauteren, I., De Smet, L., et al. (2012). Combined linkage and association mapping reveals CYCD5;1 as a quantitative trait gene for endoreduplication in Arabidopsis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(12), 4678–4683.
Vancouver
1.
Sterken R, Kiekens R, Boruc J, Zhang F, Vercauteren A, Vercauteren I, et al. Combined linkage and association mapping reveals CYCD5;1 as a quantitative trait gene for endoreduplication in Arabidopsis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2012;109(12):4678–83.
MLA
Sterken, Roel, Raphaël Kiekens, Joanna Boruc, et al. “Combined Linkage and Association Mapping Reveals CYCD5;1 as a Quantitative Trait Gene for Endoreduplication in Arabidopsis.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109.12 (2012): 4678–4683. Print.
@article{2070843,
  abstract     = {Endoreduplication is the process where a cell replicates its genome without mitosis and cytokinesis, often followed by cell differentiation. This alternative cell cycle results in various levels of endoploidy, reaching 4x or higher one haploid set of chromosomes. Endoreduplication is found in animals and is widespread in plants, where it plays a major role in cellular differentiation and plant development. Here, we show that variation in endoreduplication between Arabidopsis thaliana accessions Columbia-0 and Kashmir is controlled by two major quantitative trait loci, ENDO-1 and ENDO-2. A local candidate gene association analysis in a set of 87 accessions, combined with expression analysis, identified CYCD5;1 as the most likely candidate gene underlying ENDO-2, operating as a rate-determining factor of endoreduplication. In accordance, both the overexpression and silencing of CYCD5;1 were effective in changing DNA ploidy levels, confirming CYCD5;1 to be a previously undescribed quantitative trait gene underlying endoreduplication in Arabidopsis.},
  author       = {Sterken, Roel and Kiekens, Rapha{\"e}l and Boruc, Joanna and Zhang, Fanghong and Vercauteren, Annelies and Vercauteren, Ilse and De Smet, Lien and Dhondt, Stijn and Inz{\'e}, Dirk and De Veylder, Lieven and Russinova, Eugenia and Vuylsteke, Marnik},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {CYCLIN-DEPENDENT KINASE,regulatory haplotype,endopolyploidy,change-of-function allele,MAYS SSP PARVIGLUMIS,GENOME-WIDE ANALYSIS,CANDIDATE GENE,CELL-SIZE,PLANT DEVELOPMENT,LEAF DEVELOPMENT,ENDOCYCLE ONSET,ORGAN GROWTH,THALIANA},
  language     = {eng},
  number       = {12},
  pages        = {4678--4683},
  title        = {Combined linkage and association mapping reveals CYCD5;1 as a quantitative trait gene for endoreduplication in Arabidopsis},
  url          = {http://dx.doi.org/10.1073/pnas.1120811109},
  volume       = {109},
  year         = {2012},
}

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