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A pilot study on the use of laser ablation-ICP-mass spectrometry for assessing/mapping the distribution of a drug and its metabolites across the body compartments of rats

Andrei Izmer UGent, Deepti Sahebrao Gholap UGent, Kathleen De Houwer, Filip Cuyckens and Frank Vanhaecke UGent (2012) JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 27(3). p.413-418
abstract
Application of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) has been studied as an alternative to radioluminography (RLG) for assessing the distribution of a novel anti-tuberculosis compound containing bromine as a "hetero-element" and its metabolites over the body compartments of rat, used as a test animal. In contrast to RLG, LA-ICP-MS does not require labeling of the drug compound with a radionuclide. After administration of the Br-containing drug and a sufficient delay time to allow drug uptake and distribution, the sacrificed animal was frozen and embedded in carboxymethyl cellulose (CMC), after which thin sections were obtained using a microtome. For quantification purposes, hardened gelatin films on a glass support were used as "matrix-matched" Br standards. The limit of detection (LOD) for Br estimated using one of these in-house made standards was 0.1 mu g g(-1), which is sufficiently low to allow visualization of Br in the main organ of interest (lung). LA-ICP-MS analysis via single spot drilling with a wider laser beam diameter on the organ of interest was performed as well. In this case, quantification was accomplished via external calibration versus droplets of standards containing the pure drug that were co-embedded with the rat. Figures of merit achievable with LA-ICP-MS were critically evaluated and compared to those typical for the traditional technique, based on the use of radionuclides.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
AUTORADIOGRAPHY, ADME, DISCOVERY, SECTIONS, RADIOLUMINOGRAPHY, MS, MYCOBACTERIUM-TUBERCULOSIS
journal title
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
J. Anal. At. Spectrom.
volume
27
issue
3
pages
413 - 418
Web of Science type
Article
Web of Science id
000300316200004
JCR category
SPECTROSCOPY
JCR impact factor
3.155 (2012)
JCR rank
7/43 (2012)
JCR quartile
1 (2012)
ISSN
0267-9477
DOI
10.1039/c2ja10343e
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2070310
handle
http://hdl.handle.net/1854/LU-2070310
date created
2012-03-20 15:06:06
date last changed
2012-04-10 11:37:30
@article{2070310,
  abstract     = {Application of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) has been studied as an alternative to radioluminography (RLG) for assessing the distribution of a novel anti-tuberculosis compound containing bromine as a {\textacutedbl}hetero-element{\textacutedbl} and its metabolites over the body compartments of rat, used as a test animal. In contrast to RLG, LA-ICP-MS does not require labeling of the drug compound with a radionuclide. After administration of the Br-containing drug and a sufficient delay time to allow drug uptake and distribution, the sacrificed animal was frozen and embedded in carboxymethyl cellulose (CMC), after which thin sections were obtained using a microtome. For quantification purposes, hardened gelatin films on a glass support were used as {\textacutedbl}matrix-matched{\textacutedbl} Br standards. The limit of detection (LOD) for Br estimated using one of these in-house made standards was 0.1 mu g g(-1), which is sufficiently low to allow visualization of Br in the main organ of interest (lung). LA-ICP-MS analysis via single spot drilling with a wider laser beam diameter on the organ of interest was performed as well. In this case, quantification was accomplished via external calibration versus droplets of standards containing the pure drug that were co-embedded with the rat. Figures of merit achievable with LA-ICP-MS were critically evaluated and compared to those typical for the traditional technique, based on the use of radionuclides.},
  author       = {Izmer, Andrei and Gholap, Deepti Sahebrao and De Houwer, Kathleen and Cuyckens, Filip and Vanhaecke, Frank},
  issn         = {0267-9477},
  journal      = {JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY},
  keyword      = {AUTORADIOGRAPHY,ADME,DISCOVERY,SECTIONS,RADIOLUMINOGRAPHY,MS,MYCOBACTERIUM-TUBERCULOSIS},
  language     = {eng},
  number       = {3},
  pages        = {413--418},
  title        = {A pilot study on the use of laser ablation-ICP-mass spectrometry for assessing/mapping the distribution of a drug and its metabolites across the body compartments of rats},
  url          = {http://dx.doi.org/10.1039/c2ja10343e},
  volume       = {27},
  year         = {2012},
}

Chicago
Izmer, Andrei, Deepti Sahebrao Gholap, Kathleen De Houwer, Filip Cuyckens, and Frank Vanhaecke. 2012. “A Pilot Study on the Use of Laser ablation-ICP-mass Spectrometry for Assessing/mapping the Distribution of a Drug and Its Metabolites Across the Body Compartments of Rats.” Journal of Analytical Atomic Spectrometry 27 (3): 413–418.
APA
Izmer, A., Gholap, D. S., De Houwer, K., Cuyckens, F., & Vanhaecke, F. (2012). A pilot study on the use of laser ablation-ICP-mass spectrometry for assessing/mapping the distribution of a drug and its metabolites across the body compartments of rats. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 27(3), 413–418.
Vancouver
1.
Izmer A, Gholap DS, De Houwer K, Cuyckens F, Vanhaecke F. A pilot study on the use of laser ablation-ICP-mass spectrometry for assessing/mapping the distribution of a drug and its metabolites across the body compartments of rats. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 2012;27(3):413–8.
MLA
Izmer, Andrei, Deepti Sahebrao Gholap, Kathleen De Houwer, et al. “A Pilot Study on the Use of Laser ablation-ICP-mass Spectrometry for Assessing/mapping the Distribution of a Drug and Its Metabolites Across the Body Compartments of Rats.” JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 27.3 (2012): 413–418. Print.