Ghent University Academic Bibliography

Advanced

Speciation analysis of bromine-containing drug metabolites in feces samples from a human in vivo study by means of HPLC/ICP-MS combined with on-line isotope dilution

Björn Meermann UGent, Marc Bockx, Aline Laenen, Cis Van Looveren, Filip Cuyckens and Frank Vanhaecke UGent (2012) ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 402(1). p.439-448
abstract
The aim of this work was speciation analysis of metabolites in feces samples collected within a clinical study during which a bromine-containing anti-tuberculosis drug (TMC207) was administered to patients with multi-drug resistant tuberculosis infection. Owing to slow elimination of the drug, no (14)C label was used within this study. Quantification of the bromine species was accomplished using high performance liquid chromatography coupled to inductively coupled plasma-mass spectrometry (HPLC/ICP-MS) in combination with on-line isotope dilution (on-line ID), while structural elucidation of the species was performed using HPLC coupled to electrospray ionization-mass spectrometry. The ICP-MS-based method developed shows a good intra- and inter-day reproducibility (relative standard deviation = 3.5%, N = 9); the limit of detection (1.5 mg TMC207 L(-1)) is of the same order of magnitude as that for HPLC/radiodetection; the dynamic range of the method covers more than two orders of magnitude. Furthermore, the column recovery was demonstrated to be quantitative (recoveries between 90.6% and 99.5%). Based on the excellent figures of merit, the "cold" HPLC/ICP-MS approach could be deployed for the actual human in vivo metabolism study, such that exposure of the human volunteers to the (14)C radiolabel was avoided.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HPLC/ICP-MS, Post-column on-line isotope dilution, Bromine speciation analysis, ESI-MS, Human in vivo study, PLASMA-MASS SPECTROMETRY, LIQUID-CHROMATOGRAPHY, ICP-MS, QUANTIFICATION, HYPHENATION
journal title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Anal. Bioanal. Chem.
volume
402
issue
1
pages
439 - 448
Web of Science type
Article
Web of Science id
000298749300048
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.659 (2012)
JCR rank
9/74 (2012)
JCR quartile
1 (2012)
ISSN
1618-2642
DOI
10.1007/s00216-011-5339-y
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2070263
handle
http://hdl.handle.net/1854/LU-2070263
date created
2012-03-20 14:56:21
date last changed
2015-06-17 09:59:46
@article{2070263,
  abstract     = {The aim of this work was speciation analysis of metabolites in feces samples collected within a clinical study during which a bromine-containing anti-tuberculosis drug (TMC207) was administered to patients with multi-drug resistant tuberculosis infection. Owing to slow elimination of the drug, no (14)C label was used within this study. Quantification of the bromine species was accomplished using high performance liquid chromatography coupled to inductively coupled plasma-mass spectrometry (HPLC/ICP-MS) in combination with on-line isotope dilution (on-line ID), while structural elucidation of the species was performed using HPLC coupled to electrospray ionization-mass spectrometry. The ICP-MS-based method developed shows a good intra- and inter-day reproducibility (relative standard deviation = 3.5\%, N = 9); the limit of detection (1.5 mg TMC207 L(-1)) is of the same order of magnitude as that for HPLC/radiodetection; the dynamic range of the method covers more than two orders of magnitude. Furthermore, the column recovery was demonstrated to be quantitative (recoveries between 90.6\% and 99.5\%). Based on the excellent figures of merit, the {\textacutedbl}cold{\textacutedbl} HPLC/ICP-MS approach could be deployed for the actual human in vivo metabolism study, such that exposure of the human volunteers to the (14)C radiolabel was avoided.},
  author       = {Meermann, Bj{\"o}rn and Bockx, Marc and Laenen, Aline and Van Looveren, Cis and Cuyckens, Filip and Vanhaecke, Frank},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {HPLC/ICP-MS,Post-column on-line isotope dilution,Bromine speciation analysis,ESI-MS,Human in vivo study,PLASMA-MASS SPECTROMETRY,LIQUID-CHROMATOGRAPHY,ICP-MS,QUANTIFICATION,HYPHENATION},
  language     = {eng},
  number       = {1},
  pages        = {439--448},
  title        = {Speciation analysis of bromine-containing drug metabolites in feces samples from a human in vivo study by means of HPLC/ICP-MS combined with on-line isotope dilution},
  url          = {http://dx.doi.org/10.1007/s00216-011-5339-y},
  volume       = {402},
  year         = {2012},
}

Chicago
Meermann, Björn, Marc Bockx, Aline Laenen, Cis Van Looveren, Filip Cuyckens, and Frank Vanhaecke. 2012. “Speciation Analysis of Bromine-containing Drug Metabolites in Feces Samples from a Human in Vivo Study by Means of HPLC/ICP-MS Combined with On-line Isotope Dilution.” Analytical and Bioanalytical Chemistry 402 (1): 439–448.
APA
Meermann, B., Bockx, M., Laenen, A., Van Looveren, C., Cuyckens, F., & Vanhaecke, F. (2012). Speciation analysis of bromine-containing drug metabolites in feces samples from a human in vivo study by means of HPLC/ICP-MS combined with on-line isotope dilution. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 402(1), 439–448.
Vancouver
1.
Meermann B, Bockx M, Laenen A, Van Looveren C, Cuyckens F, Vanhaecke F. Speciation analysis of bromine-containing drug metabolites in feces samples from a human in vivo study by means of HPLC/ICP-MS combined with on-line isotope dilution. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2012;402(1):439–48.
MLA
Meermann, Björn, Marc Bockx, Aline Laenen, et al. “Speciation Analysis of Bromine-containing Drug Metabolites in Feces Samples from a Human in Vivo Study by Means of HPLC/ICP-MS Combined with On-line Isotope Dilution.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 402.1 (2012): 439–448. Print.