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Hypericin-based photodynamic therapy induces surface exposure of damage-associated molecular patterns like HSP70 and calreticulin

Abhishek D Garg, Dmitri Krysko UGent, Peter Vandenabeele UGent and Patrizia Agostinis (2012) CANCER IMMUNOLOGY IMMUNOTHERAPY. 61(2). p.215-221
abstract
Surface-exposed HSP70 and calreticulin are damage-associated molecular patterns (DAMPs) crucially involved in modulating the success of cancer therapy. Photodynamic therapy (PDT) involves the administration of a photosensitising (PTS) agent followed by visible light-irradiation. The reactive oxygen species that are thus generated directly kill tumours by damaging their microvasculature and inducing a local inflammatory reaction. PDT with the PTS photofrin is associated with DAMPs exposure, but the same is not true for other PTSs. Here, we show that when cancer cells are treated with hypericin-based PDT (Hyp-PDT), they surface-expose both HSP70 and calreticulin (CRT). Induction of CRT exposure was not accompanied by co-exposure of ERp57, but this did not compromise the ability of the exposed CRT to regulate the phagocytosis of Hyp-PDT-treated cancer cells by dendritic cells. Interestingly, we found that Hyp-PDT-induced CRT exposure (in contrast to anthracycline-induced CRT exposure) was independent of the presence of ERp57. Our results indicate that Hyp-PDT is a potential anti-cancer immunogenic modality.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
EXPRESSION, TRANSLOCATION, IMMUNOGENICITY, APOPTOTIC-CELLS, TUMOR-CELL DEATH, Cancer, Hypericin, Photodynamic therapy, HSP70, Calreticulin, DAMPs, IMMUNITY, CANCER, PHAGOCYTOSIS, INVOLVEMENT, ACTIVATION
journal title
CANCER IMMUNOLOGY IMMUNOTHERAPY
Cancer Immunol. Immunother.
volume
61
issue
2
pages
215 - 221
Web of Science type
Article
Web of Science id
000300662200007
JCR category
ONCOLOGY
JCR impact factor
3.637 (2012)
JCR rank
60/196 (2012)
JCR quartile
2 (2012)
ISSN
0340-7004
DOI
10.1007/s00262-011-1184-2
project
Ghent researchers on unfolded proteins in inflammatory disease (GROUP-ID)
project
Ghent researchers on unfolded proteins in inflammatory disease (GROUP-ID)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2067218
handle
http://hdl.handle.net/1854/LU-2067218
date created
2012-03-15 13:19:01
date last changed
2014-05-12 10:59:04
@article{2067218,
  abstract     = {Surface-exposed HSP70 and calreticulin are damage-associated molecular patterns (DAMPs) crucially involved in modulating the success of cancer therapy. Photodynamic therapy (PDT) involves the administration of a photosensitising (PTS) agent followed by visible light-irradiation. The reactive oxygen species that are thus generated directly kill tumours by damaging their microvasculature and inducing a local inflammatory reaction. PDT with the PTS photofrin is associated with DAMPs exposure, but the same is not true for other PTSs. Here, we show that when cancer cells are treated with hypericin-based PDT (Hyp-PDT), they surface-expose both HSP70 and calreticulin (CRT). Induction of CRT exposure was not accompanied by co-exposure of ERp57, but this did not compromise the ability of the exposed CRT to regulate the phagocytosis of Hyp-PDT-treated cancer cells by dendritic cells. Interestingly, we found that Hyp-PDT-induced CRT exposure (in contrast to anthracycline-induced CRT exposure) was independent of the presence of ERp57. Our results indicate that Hyp-PDT is a potential anti-cancer immunogenic modality.},
  author       = {Garg, Abhishek D and Krysko, Dmitri and Vandenabeele, Peter and Agostinis, Patrizia},
  issn         = {0340-7004},
  journal      = {CANCER IMMUNOLOGY IMMUNOTHERAPY},
  keyword      = {EXPRESSION,TRANSLOCATION,IMMUNOGENICITY,APOPTOTIC-CELLS,TUMOR-CELL DEATH,Cancer,Hypericin,Photodynamic therapy,HSP70,Calreticulin,DAMPs,IMMUNITY,CANCER,PHAGOCYTOSIS,INVOLVEMENT,ACTIVATION},
  language     = {eng},
  number       = {2},
  pages        = {215--221},
  title        = {Hypericin-based photodynamic therapy induces surface exposure of damage-associated molecular patterns like HSP70 and calreticulin},
  url          = {http://dx.doi.org/10.1007/s00262-011-1184-2},
  volume       = {61},
  year         = {2012},
}

Chicago
Garg, Abhishek D, Dmitri Krysko, Peter Vandenabeele, and Patrizia Agostinis. 2012. “Hypericin-based Photodynamic Therapy Induces Surface Exposure of Damage-associated Molecular Patterns Like HSP70 and Calreticulin.” Cancer Immunology Immunotherapy 61 (2): 215–221.
APA
Garg, Abhishek D, Krysko, D., Vandenabeele, P., & Agostinis, P. (2012). Hypericin-based photodynamic therapy induces surface exposure of damage-associated molecular patterns like HSP70 and calreticulin. CANCER IMMUNOLOGY IMMUNOTHERAPY, 61(2), 215–221.
Vancouver
1.
Garg AD, Krysko D, Vandenabeele P, Agostinis P. Hypericin-based photodynamic therapy induces surface exposure of damage-associated molecular patterns like HSP70 and calreticulin. CANCER IMMUNOLOGY IMMUNOTHERAPY. 2012;61(2):215–21.
MLA
Garg, Abhishek D, Dmitri Krysko, Peter Vandenabeele, et al. “Hypericin-based Photodynamic Therapy Induces Surface Exposure of Damage-associated Molecular Patterns Like HSP70 and Calreticulin.” CANCER IMMUNOLOGY IMMUNOTHERAPY 61.2 (2012): 215–221. Print.