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Effect of challenge of pigs previously immunised with inactivated vaccines containing homologous and heterologous Mycoplasma hyopneumoniae strains

Iris Villarreal UGent, Katleen Vranckx UGent, Dries Calus UGent, Frank Pasmans UGent, Freddy Haesebrouck UGent and Dominiek Maes UGent (2012) BMC VETERINARY RESEARCH. 8.
abstract
Background: Mycoplasma hyopneumoniae is the primary cause of enzootic pneumonia in pigs. Although vaccination is an important control tool, the results observed under field conditions are variable. This may be due to antigenic differences between the strains circulating in pig herds and the vaccine strain. This study compared the protective efficacy of four bacterins against challenge infection with a highly virulent field strain of M. hyopneumoniae. Seventy eight, one-week old piglets were randomly assigned to five treatment groups (A, B, C, D, E), 14 piglets each, and a negative control group (F) consisting of 8 piglets. All pigs were injected at 1 (D7) and 4 weeks of age (D28), with 2 ml of either a placebo or a bacterin based on selected M. hyopneumoniae strains, namely A (F7.2C), B (F20.1L), C (B2V1W20 1A-F), D (J strain), E (placebo; positive control), F (placebo; negative control). At D56, all pigs except those of group F were challenged intratracheally with 7 ml culture medium containing 107 CCU/ml of M. hyopneumoniae strain F7.2C. All pigs were euthanized and necropsied at D84. The severity of coughing and pneumonia lesions were the main parameters. Immunofluorescence (IF) testing, nested PCR testing of bronchoalveolar lavage (BAL) fluid and serology for M. hyopneumoniae were also performed. Results: The different bacterins only slightly improved clinical symptoms (average 0.38 in vaccinated groups vs. 0.45 in group E) and histopathological lung lesions (average 3.20 in vaccinated groups vs. 3.45 in group E), but did not improve macroscopic lung lesions (score 4.30 vs. 4.03 in group E). None of the vaccines was significantly and/or consistently better or worse than the other ones. All bacterins evoked a serological response in the vaccinated animals. All pigs, except those from group F, were positive with nPCR in BAL fluid at D84. Conclusion: The bacterins did not induce a clear overall protection against challenge infection, and there were no significant differences in protective efficacy between bacterins containing homologous and heterologous M. hyopneumoniae strains. Further research is necessary to better characterize the antigens involved in protection and to elucidate the protective immunity responses following M. hyopneumoniae vaccination and/or infection.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ENZOOTIC PNEUMONIA, RESPIRATORY SYNDROME VIRUS, SUIPNEUMONIAE, INFECTIONS, PROTECTION, RESPONSES, TYLOSIN
journal title
BMC VETERINARY RESEARCH
BMC Vet. Res.
volume
8
article_number
2
pages
7 pages
Web of Science type
Article
Web of Science id
000300297300001
JCR category
VETERINARY SCIENCES
JCR impact factor
1.861 (2012)
JCR rank
18/142 (2012)
JCR quartile
1 (2012)
ISSN
1746-6148
DOI
10.1186/1746-6148-8-2
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2065319
handle
http://hdl.handle.net/1854/LU-2065319
date created
2012-03-13 13:51:15
date last changed
2012-04-05 14:35:50
@article{2065319,
  abstract     = {Background: Mycoplasma hyopneumoniae is the primary cause of enzootic pneumonia in pigs. Although vaccination is an important control tool, the results observed under field conditions are variable. This may be due to antigenic differences between the strains circulating in pig herds and the vaccine strain. This study compared the protective efficacy of four bacterins against challenge infection with a highly virulent field strain of M. hyopneumoniae. 
Seventy eight, one-week old piglets were randomly assigned to five treatment groups (A, B, C, D, E), 14 piglets each, and a negative control group (F) consisting of 8 piglets. All pigs were injected at 1 (D7) and 4 weeks of age (D28), with 2 ml of either a placebo or a bacterin based on selected M. hyopneumoniae strains, namely A (F7.2C), B (F20.1L), C (B2V1W20 1A-F), D (J strain), E (placebo; positive control), F (placebo; negative control). At D56, all pigs except those of group F were challenged intratracheally with 7 ml culture medium containing 107 CCU/ml of M. hyopneumoniae strain F7.2C. All pigs were euthanized and necropsied at D84. The severity of coughing and pneumonia lesions were the main parameters. Immunofluorescence (IF) testing, nested PCR testing of bronchoalveolar lavage (BAL) fluid and serology for M. hyopneumoniae were also performed. 
Results: The different bacterins only slightly improved clinical symptoms (average 0.38 in vaccinated groups vs. 0.45 in group E) and histopathological lung lesions (average 3.20 in vaccinated groups vs. 3.45 in group E), but did not improve macroscopic lung lesions (score 4.30 vs. 4.03 in group E). None of the vaccines was significantly and/or consistently better or worse than the other ones. All bacterins evoked a serological response in the vaccinated animals. All pigs, except those from group F, were positive with nPCR in BAL fluid at D84. 
Conclusion: The bacterins did not induce a clear overall protection against challenge infection, and there were no significant differences in protective efficacy between bacterins containing homologous and heterologous M. hyopneumoniae strains. Further research is necessary to better characterize the antigens involved in protection and to elucidate the protective immunity responses following M. hyopneumoniae vaccination and/or infection.},
  articleno    = {2},
  author       = {Villarreal, Iris and Vranckx, Katleen and Calus, Dries and Pasmans, Frank and Haesebrouck, Freddy and Maes, Dominiek},
  issn         = {1746-6148},
  journal      = {BMC VETERINARY RESEARCH},
  keyword      = {ENZOOTIC PNEUMONIA,RESPIRATORY SYNDROME VIRUS,SUIPNEUMONIAE,INFECTIONS,PROTECTION,RESPONSES,TYLOSIN},
  language     = {eng},
  pages        = {7},
  title        = {Effect of challenge of pigs previously immunised with inactivated vaccines containing homologous and heterologous Mycoplasma hyopneumoniae strains},
  url          = {http://dx.doi.org/10.1186/1746-6148-8-2},
  volume       = {8},
  year         = {2012},
}

Chicago
Villarreal, Iris, Katleen Vranckx, Dries Calus, Frank Pasmans, Freddy Haesebrouck, and Dominiek Maes. 2012. “Effect of Challenge of Pigs Previously Immunised with Inactivated Vaccines Containing Homologous and Heterologous Mycoplasma Hyopneumoniae Strains.” Bmc Veterinary Research 8.
APA
Villarreal, I., Vranckx, K., Calus, D., Pasmans, F., Haesebrouck, F., & Maes, D. (2012). Effect of challenge of pigs previously immunised with inactivated vaccines containing homologous and heterologous Mycoplasma hyopneumoniae strains. BMC VETERINARY RESEARCH, 8.
Vancouver
1.
Villarreal I, Vranckx K, Calus D, Pasmans F, Haesebrouck F, Maes D. Effect of challenge of pigs previously immunised with inactivated vaccines containing homologous and heterologous Mycoplasma hyopneumoniae strains. BMC VETERINARY RESEARCH. 2012;8.
MLA
Villarreal, Iris, Katleen Vranckx, Dries Calus, et al. “Effect of Challenge of Pigs Previously Immunised with Inactivated Vaccines Containing Homologous and Heterologous Mycoplasma Hyopneumoniae Strains.” BMC VETERINARY RESEARCH 8 (2012): n. pag. Print.