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Complete genome characterization of a East European Type 1 subtype 3 porcine reproductive and respiratory syndrome virus

(2012) VIRUS GENES. 44(1). p.51-54
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Abstract
Porcine reproductive and respiratory syndrome (PRRS) is a swine disease of major economic importance that causes reproductive and respiratory problems in pigs. PRRSV strains are divided into European (Type 1) and North-American (Type 2) genotypes. Within the European PRRSV genotype, three subtypes have been delineated. Full genome sequences for North American and European subtype 1 strains have been described. Here, the first complete genomic characterization of a European subtype 3 strain (Lena) is described. Amplification of Orf1a and Orf1b fragments was achieved using a set of degenerate oligonucleotides. Using RT-PCR with Lena-specific primers, the full length sequence (15001 nt) was obtained. Alignment of Lena with European subtype 1 reference strain Lelystad showed variation over the entire length (84% identity/89% similarity at amino acid level) with the most variation in Orf1a (Nsp2/NSP2) with a deletion of 29 amino acids. Phylogenetic relationships using different Orfs supported Lena's genetic distinction from European subtype 1 strains. The availability of the European subtype 3 PRRSV full genome may be important for the understanding of PRRSV evolution and the more pronounced pathogenic nature of Lena.
Keywords
Phylogenetic analysis, LELYSTAD VIRUS, Full genome sequencing, RT-PCR, PRRSV, STRAINS, DEFINITION, DIVERSITY, PROTEIN, ISOLATE

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Chicago
Van Doorsselaere, Jan, MS Brar, M Shi, Uladzimir Karniychuk, FCC Leung, and Hans Nauwynck. 2012. “Complete Genome Characterization of a East European Type 1 Subtype 3 Porcine Reproductive and Respiratory Syndrome Virus.” Virus Genes 44 (1): 51–54.
APA
Van Doorsselaere, Jan, Brar, M., Shi, M., Karniychuk, U., Leung, F., & Nauwynck, H. (2012). Complete genome characterization of a East European Type 1 subtype 3 porcine reproductive and respiratory syndrome virus. VIRUS GENES, 44(1), 51–54.
Vancouver
1.
Van Doorsselaere J, Brar M, Shi M, Karniychuk U, Leung F, Nauwynck H. Complete genome characterization of a East European Type 1 subtype 3 porcine reproductive and respiratory syndrome virus. VIRUS GENES. 2012;44(1):51–4.
MLA
Van Doorsselaere, Jan, MS Brar, M Shi, et al. “Complete Genome Characterization of a East European Type 1 Subtype 3 Porcine Reproductive and Respiratory Syndrome Virus.” VIRUS GENES 44.1 (2012): 51–54. Print.
@article{2064332,
  abstract     = {Porcine reproductive and respiratory syndrome (PRRS) is a swine disease of major economic importance that causes reproductive and respiratory problems in pigs. PRRSV strains are divided into European (Type 1) and North-American (Type 2) genotypes. Within the European PRRSV genotype, three subtypes have been delineated. Full genome sequences for North American and European subtype 1 strains have been described. Here, the first complete genomic characterization of a European subtype 3 strain (Lena) is described. Amplification of Orf1a and Orf1b fragments was achieved using a set of degenerate oligonucleotides. Using RT-PCR with Lena-specific primers, the full length sequence (15001 nt) was obtained. Alignment of Lena with European subtype 1 reference strain Lelystad showed variation over the entire length (84\% identity/89\% similarity at amino acid level) with the most variation in Orf1a (Nsp2/NSP2) with a deletion of 29 amino acids. Phylogenetic relationships using different Orfs supported Lena's genetic distinction from European subtype 1 strains. The availability of the European subtype 3 PRRSV full genome may be important for the understanding of PRRSV evolution and the more pronounced pathogenic nature of Lena.},
  author       = {Van Doorsselaere, Jan and Brar, MS and Shi, M and Karniychuk, Uladzimir and Leung, FCC and Nauwynck, Hans},
  issn         = {0920-8569},
  journal      = {VIRUS GENES},
  keyword      = {Phylogenetic analysis,LELYSTAD VIRUS,Full genome sequencing,RT-PCR,PRRSV,STRAINS,DEFINITION,DIVERSITY,PROTEIN,ISOLATE},
  language     = {eng},
  number       = {1},
  pages        = {51--54},
  title        = {Complete genome characterization of a East European Type 1 subtype 3 porcine reproductive and respiratory syndrome virus},
  url          = {http://dx.doi.org/10.1007/s11262-011-0665-x},
  volume       = {44},
  year         = {2012},
}

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