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Complete genome characterization of a East European Type 1 subtype 3 porcine reproductive and respiratory syndrome virus

Jan Van Doorsselaere UGent, MS Brar, M Shi, Uladzimir Karniychuk UGent, FCC Leung and Hans Nauwynck UGent (2012) VIRUS GENES. 44(1). p.51-54
abstract
Porcine reproductive and respiratory syndrome (PRRS) is a swine disease of major economic importance that causes reproductive and respiratory problems in pigs. PRRSV strains are divided into European (Type 1) and North-American (Type 2) genotypes. Within the European PRRSV genotype, three subtypes have been delineated. Full genome sequences for North American and European subtype 1 strains have been described. Here, the first complete genomic characterization of a European subtype 3 strain (Lena) is described. Amplification of Orf1a and Orf1b fragments was achieved using a set of degenerate oligonucleotides. Using RT-PCR with Lena-specific primers, the full length sequence (15001 nt) was obtained. Alignment of Lena with European subtype 1 reference strain Lelystad showed variation over the entire length (84% identity/89% similarity at amino acid level) with the most variation in Orf1a (Nsp2/NSP2) with a deletion of 29 amino acids. Phylogenetic relationships using different Orfs supported Lena's genetic distinction from European subtype 1 strains. The availability of the European subtype 3 PRRSV full genome may be important for the understanding of PRRSV evolution and the more pronounced pathogenic nature of Lena.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Phylogenetic analysis, LELYSTAD VIRUS, Full genome sequencing, RT-PCR, PRRSV, STRAINS, DEFINITION, DIVERSITY, PROTEIN, ISOLATE
journal title
VIRUS GENES
Virus Genes
volume
44
issue
1
pages
51 - 54
Web of Science type
Article
Web of Science id
000299510900008
JCR category
GENETICS & HEREDITY
JCR impact factor
1.769 (2012)
JCR rank
118/161 (2012)
JCR quartile
3 (2012)
ISSN
0920-8569
DOI
10.1007/s11262-011-0665-x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2064332
handle
http://hdl.handle.net/1854/LU-2064332
date created
2012-03-12 11:55:16
date last changed
2012-04-06 16:20:24
@article{2064332,
  abstract     = {Porcine reproductive and respiratory syndrome (PRRS) is a swine disease of major economic importance that causes reproductive and respiratory problems in pigs. PRRSV strains are divided into European (Type 1) and North-American (Type 2) genotypes. Within the European PRRSV genotype, three subtypes have been delineated. Full genome sequences for North American and European subtype 1 strains have been described. Here, the first complete genomic characterization of a European subtype 3 strain (Lena) is described. Amplification of Orf1a and Orf1b fragments was achieved using a set of degenerate oligonucleotides. Using RT-PCR with Lena-specific primers, the full length sequence (15001 nt) was obtained. Alignment of Lena with European subtype 1 reference strain Lelystad showed variation over the entire length (84\% identity/89\% similarity at amino acid level) with the most variation in Orf1a (Nsp2/NSP2) with a deletion of 29 amino acids. Phylogenetic relationships using different Orfs supported Lena's genetic distinction from European subtype 1 strains. The availability of the European subtype 3 PRRSV full genome may be important for the understanding of PRRSV evolution and the more pronounced pathogenic nature of Lena.},
  author       = {Van Doorsselaere, Jan and Brar, MS and Shi, M and Karniychuk, Uladzimir and Leung, FCC and Nauwynck, Hans},
  issn         = {0920-8569},
  journal      = {VIRUS GENES},
  keyword      = {Phylogenetic analysis,LELYSTAD VIRUS,Full genome sequencing,RT-PCR,PRRSV,STRAINS,DEFINITION,DIVERSITY,PROTEIN,ISOLATE},
  language     = {eng},
  number       = {1},
  pages        = {51--54},
  title        = {Complete genome characterization of a East European Type 1 subtype 3 porcine reproductive and respiratory syndrome virus},
  url          = {http://dx.doi.org/10.1007/s11262-011-0665-x},
  volume       = {44},
  year         = {2012},
}

Chicago
Van Doorsselaere, Jan, MS Brar, M Shi, Uladzimir Karniychuk, FCC Leung, and Hans Nauwynck. 2012. “Complete Genome Characterization of a East European Type 1 Subtype 3 Porcine Reproductive and Respiratory Syndrome Virus.” Virus Genes 44 (1): 51–54.
APA
Van Doorsselaere, Jan, Brar, M., Shi, M., Karniychuk, U., Leung, F., & Nauwynck, H. (2012). Complete genome characterization of a East European Type 1 subtype 3 porcine reproductive and respiratory syndrome virus. VIRUS GENES, 44(1), 51–54.
Vancouver
1.
Van Doorsselaere J, Brar M, Shi M, Karniychuk U, Leung F, Nauwynck H. Complete genome characterization of a East European Type 1 subtype 3 porcine reproductive and respiratory syndrome virus. VIRUS GENES. 2012;44(1):51–4.
MLA
Van Doorsselaere, Jan, MS Brar, M Shi, et al. “Complete Genome Characterization of a East European Type 1 Subtype 3 Porcine Reproductive and Respiratory Syndrome Virus.” VIRUS GENES 44.1 (2012): 51–54. Print.