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The OPFOS microscopy family: high-resolution optical-sectioning of biomedical specimens

Jan AN Buytaert, Emilie Descamps UGent, Dominique Adriaens UGent and Joris JJ Dirckx (2012) ANATOMY RESEARCH INTERNATIONAL. 2012.
abstract
We report on the recently emerging (laser) light-sheet-based fluorescence microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects nondestructively in high resolution through virtual optical sectioning with sheets of laser light. Fluorescence originating in the cross-section of the sheet and sample is recorded orthogonally with a camera. In this paper, the first implementation of LSFM to image biomedical tissue in three dimensions—orthogonal-plane fluorescence optical sectioning microscopy (OPFOS)—is discussed. Since then many similar and derived methods have surfaced, (SPIM, ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM, etc.) which we all briefly discuss. All these optical sectioning methods create images showing histological detail. We illustrate the applicability of LSFM on several specimen types with application in biomedical and life sciences.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (review)
publication status
published
subject
journal title
ANATOMY RESEARCH INTERNATIONAL
Anatomy Research International
volume
2012
article_number
206238
pages
9 pages
ISSN
2090-2743
DOI
10.1155/2012/206238
language
English
UGent publication?
yes
classification
A2
copyright statement
I have retained and own the full copyright for this publication
id
2052759
handle
http://hdl.handle.net/1854/LU-2052759
alternative location
http://www.evomorph.ugent.be/Publications/Publ100.pdf
date created
2012-02-29 16:40:54
date last changed
2012-04-04 12:55:34
@article{2052759,
  abstract     = {We report on the recently emerging (laser) light-sheet-based fluorescence microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects nondestructively in high resolution through virtual optical sectioning with sheets of laser light. Fluorescence originating in the cross-section of the sheet and sample is recorded orthogonally with a camera.
In this paper, the first implementation of LSFM to image biomedical tissue in three dimensions---orthogonal-plane fluorescence optical sectioning microscopy (OPFOS)---is discussed. Since then many similar and derived methods have surfaced, (SPIM, ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM, etc.) which we all briefly discuss. All these optical sectioning methods create images showing histological detail. We illustrate the applicability of LSFM on several specimen types with application in biomedical and life sciences.},
  articleno    = {206238},
  author       = {Buytaert, Jan AN and Descamps, Emilie and Adriaens, Dominique and Dirckx, Joris JJ},
  issn         = {2090-2743},
  journal      = {ANATOMY RESEARCH INTERNATIONAL},
  language     = {eng},
  pages        = {9},
  title        = {The OPFOS microscopy family: high-resolution optical-sectioning of biomedical specimens},
  url          = {http://dx.doi.org/10.1155/2012/206238},
  volume       = {2012},
  year         = {2012},
}

Chicago
Buytaert, Jan AN, Emilie Descamps, Dominique Adriaens, and Joris JJ Dirckx. 2012. “The OPFOS Microscopy Family: High-resolution Optical-sectioning of Biomedical Specimens.” Anatomy Research International 2012.
APA
Buytaert, J. A., Descamps, E., Adriaens, D., & Dirckx, J. J. (2012). The OPFOS microscopy family: high-resolution optical-sectioning of biomedical specimens. ANATOMY RESEARCH INTERNATIONAL, 2012.
Vancouver
1.
Buytaert JA, Descamps E, Adriaens D, Dirckx JJ. The OPFOS microscopy family: high-resolution optical-sectioning of biomedical specimens. ANATOMY RESEARCH INTERNATIONAL. 2012;2012.
MLA
Buytaert, Jan AN, Emilie Descamps, Dominique Adriaens, et al. “The OPFOS Microscopy Family: High-resolution Optical-sectioning of Biomedical Specimens.” ANATOMY RESEARCH INTERNATIONAL 2012 (2012): n. pag. Print.