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The OPFOS microscopy family: high-resolution optical-sectioning of biomedical specimens

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Abstract
We report on the recently emerging (laser) light-sheet-based fluorescence microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects nondestructively in high resolution through virtual optical sectioning with sheets of laser light. Fluorescence originating in the cross-section of the sheet and sample is recorded orthogonally with a camera. In this paper, the first implementation of LSFM to image biomedical tissue in three dimensions—orthogonal-plane fluorescence optical sectioning microscopy (OPFOS)—is discussed. Since then many similar and derived methods have surfaced, (SPIM, ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM, etc.) which we all briefly discuss. All these optical sectioning methods create images showing histological detail. We illustrate the applicability of LSFM on several specimen types with application in biomedical and life sciences.

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Chicago
Buytaert, Jan AN, Emilie Descamps, Dominique Adriaens, and Joris JJ Dirckx. 2012. “The OPFOS Microscopy Family: High-resolution Optical-sectioning of Biomedical Specimens.” Anatomy Research International 2012.
APA
Buytaert, J. A., Descamps, E., Adriaens, D., & Dirckx, J. J. (2012). The OPFOS microscopy family: high-resolution optical-sectioning of biomedical specimens. ANATOMY RESEARCH INTERNATIONAL, 2012.
Vancouver
1.
Buytaert JA, Descamps E, Adriaens D, Dirckx JJ. The OPFOS microscopy family: high-resolution optical-sectioning of biomedical specimens. ANATOMY RESEARCH INTERNATIONAL. 2012;2012.
MLA
Buytaert, Jan AN, Emilie Descamps, Dominique Adriaens, et al. “The OPFOS Microscopy Family: High-resolution Optical-sectioning of Biomedical Specimens.” ANATOMY RESEARCH INTERNATIONAL 2012 (2012): n. pag. Print.
@article{2052759,
  abstract     = {We report on the recently emerging (laser) light-sheet-based fluorescence microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects nondestructively in high resolution through virtual optical sectioning with sheets of laser light. Fluorescence originating in the cross-section of the sheet and sample is recorded orthogonally with a camera.
In this paper, the first implementation of LSFM to image biomedical tissue in three dimensions---orthogonal-plane fluorescence optical sectioning microscopy (OPFOS)---is discussed. Since then many similar and derived methods have surfaced, (SPIM, ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM, etc.) which we all briefly discuss. All these optical sectioning methods create images showing histological detail. We illustrate the applicability of LSFM on several specimen types with application in biomedical and life sciences.},
  articleno    = {206238},
  author       = {Buytaert, Jan AN and Descamps, Emilie and Adriaens, Dominique and Dirckx, Joris JJ},
  issn         = {2090-2743},
  journal      = {ANATOMY RESEARCH INTERNATIONAL},
  language     = {eng},
  pages        = {9},
  title        = {The OPFOS microscopy family: high-resolution optical-sectioning of biomedical specimens},
  url          = {http://dx.doi.org/10.1155/2012/206238},
  volume       = {2012},
  year         = {2012},
}

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