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Fast quantification of THCA using microwave-accelerated derivatization and GC-MS/MS analysis

Nik De Brabanter (UGent) , Wim Van Gansbeke (UGent) , Fiona Hooghe (UGent) and Peter Van Eenoo (UGent)
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Abstract
A rapid and sensitive determination of cannabinoids in urine is important in many fields, from workplace drug testing over toxicology to the fight against doping. The detection of cannabis abuse is normally based on the quantification of the most important metabolite 11-nor-∆9-tetrahydrocannabinol-9-carbolxylic acid (THCA) in urine. In most fields THCA needs to be present at a concentration of exceeding 15 ng/mL before a positive result can be reported. In this paper a fast confirmation method is described to quantify THCA in 1 mL of urine. This method combines a 4 min GC-QqQ-MS method with a fast sample preparation procedure using microwave assisted derivatisation in order to complete the quantification of THCA in urine in 30 min. The method is selective, linear over the range 5 – 100 ng/mL and shows excellent precision and trueness and hence, the estimated measurement uncertainty at the threshold level is small. The method also complies with applicable criteria for mass spectrometry and chromatography. Therefore the method can be used for rapid screening and confirmatory purposes.
Keywords
doping, GC-MS/MS, 11-nor-∆9-tetrahydrocannabinol-9-carbolxylic acid (THCA), microwave-assisted derivatisation (MAD), urine

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Chicago
De Brabanter, Nik, Wim Van Gansbeke, Fiona Hooghe, and Peter Van Eenoo. 2012. “Fast Quantification of THCA Using Microwave-accelerated Derivatization and GC-MS/MS Analysis.” In Recent Advances in Doping Analysis. Vol. 20. Sportverlag Strauss.
APA
De Brabanter, N., Van Gansbeke, W., Hooghe, F., & Van Eenoo, P. (2012). Fast quantification of THCA using microwave-accelerated derivatization and GC-MS/MS analysis. Recent Advances in Doping Analysis (Vol. 20). Presented at the 30th Cologne workshop on Dope Analysis (Manfred Donike workshop), Sportverlag Strauss.
Vancouver
1.
De Brabanter N, Van Gansbeke W, Hooghe F, Van Eenoo P. Fast quantification of THCA using microwave-accelerated derivatization and GC-MS/MS analysis. Recent Advances in Doping Analysis. Sportverlag Strauss; 2012.
MLA
De Brabanter, Nik, Wim Van Gansbeke, Fiona Hooghe, et al. “Fast Quantification of THCA Using Microwave-accelerated Derivatization and GC-MS/MS Analysis.” Recent Advances in Doping Analysis. Vol. 20. Sportverlag Strauss, 2012. Print.
@inproceedings{2051321,
  abstract     = {A rapid and sensitive determination of cannabinoids in urine is important in many fields, from workplace drug testing over toxicology to the fight against doping. The detection of cannabis abuse is normally based on the quantification of the most important metabolite 11-nor-\unmatched{2206}9-tetrahydrocannabinol-9-carbolxylic acid (THCA) in urine. In most fields THCA needs to be present at a concentration of exceeding 15 ng/mL before a positive result can be reported. 
In this paper a fast confirmation method is described to quantify THCA in 1 mL of urine. This method combines a 4 min GC-QqQ-MS method with a fast sample preparation procedure using microwave assisted derivatisation in order to complete the quantification of THCA in urine in 30 min. 
The method is selective, linear over the range 5 -- 100 ng/mL and shows excellent precision and trueness and hence, the estimated measurement uncertainty at the threshold level is small. The method also complies with applicable criteria for mass spectrometry and chromatography. Therefore the method can be used for rapid screening and confirmatory purposes.},
  author       = {De Brabanter, Nik and Van Gansbeke, Wim and Hooghe, Fiona and Van Eenoo, Peter},
  booktitle    = {Recent Advances in Doping Analysis},
  isbn         = {9783868840384},
  keyword      = {doping,GC-MS/MS,11-nor-\unmatched{2206}9-tetrahydrocannabinol-9-carbolxylic acid (THCA),microwave-assisted derivatisation (MAD),urine},
  language     = {eng},
  location     = {Cologne, Germany},
  publisher    = {Sportverlag Strauss},
  title        = {Fast quantification of THCA using microwave-accelerated derivatization and GC-MS/MS analysis},
  volume       = {20},
  year         = {2012},
}