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Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from water

Viridiana Sistek, Andrée F Maheux, Maurice Boissinot, Kathy A Bernard, Philippe Cantin, Ilse Cleenwerck UGent, Paul De Vos UGent and Michel G Bergeron (2012) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 62. p.1314-1320
abstract
Three enterococcal isolates, CCRI-16620, CCRI-16986(T) and CCRI-16985(T), originating from water were characterized using morphological, biochemical and molecular taxonomic methods. 16S rRNA gene sequence analysis classified all three strains in the Enterococcus faecalis species group. The phylogenetic tree of 16S rRNA gene sequences showed that the three isolates form two separate branches. The first branch is represented by strains CCRI-16620 and CCRI-16986(T) and the second branch by strain CCRI-16985(T). Further sequence analysis of the housekeeping genes rpoA (encoding RNA polymerase alpha subunit), pheS (phenylalanyl-tRNA synthase), tufA (elongation factor Tu) and atpD (ATP synthase beta-subunit) as well as the results of amplified fragment length polymorphism (AFLP) DNA fingerprinting and DNA-DNA hybridization experiments confirmed the distinct status of these strains. Moreover, biochemical tests allowed phenotypic differentiation of the strains from the other species of the E. faecalis species group. On the basis of the results obtained, the names Enterococcus ureasiticus sp. nov. (type strain CCRI-16986(T)=CCUG 59304(T)=DSM 23328(T)=LMG 26304(T)) and Enterococcus quebecensis sp. nov. (type strain CCRI-16985(T)=CCUG 59306(T)=DSM 23327(T)=LMG 26306(T)) are proposed for the two hitherto undescribed species.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HUMAN CLINICAL SPECIMENS, Enterococcus sp. nov., IDENTIFICATION, PCR, DNA, HYBRIDIZATION, THAILAND, GENES
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
62
pages
1314 - 1320
Web of Science type
Article
Web of Science id
000306502100016
JCR category
MICROBIOLOGY
JCR impact factor
2.112 (2012)
JCR rank
68/116 (2012)
JCR quartile
3 (2012)
ISSN
1466-5026
DOI
10.1099/ijs.0.029033-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2050941
handle
http://hdl.handle.net/1854/LU-2050941
date created
2012-02-28 21:53:52
date last changed
2012-10-03 17:03:24
@article{2050941,
  abstract     = {Three enterococcal isolates, CCRI-16620, CCRI-16986(T) and CCRI-16985(T), originating from water were characterized using morphological, biochemical and molecular taxonomic methods. 16S rRNA gene sequence analysis classified all three strains in the Enterococcus faecalis species group. The phylogenetic tree of 16S rRNA gene sequences showed that the three isolates form two separate branches. The first branch is represented by strains CCRI-16620 and CCRI-16986(T) and the second branch by strain CCRI-16985(T). Further sequence analysis of the housekeeping genes rpoA (encoding RNA polymerase alpha subunit), pheS (phenylalanyl-tRNA synthase), tufA (elongation factor Tu) and atpD (ATP synthase beta-subunit) as well as the results of amplified fragment length polymorphism (AFLP) DNA fingerprinting and DNA-DNA hybridization experiments confirmed the distinct status of these strains. Moreover, biochemical tests allowed phenotypic differentiation of the strains from the other species of the E. faecalis species group. On the basis of the results obtained, the names Enterococcus ureasiticus sp. nov. (type strain CCRI-16986(T)=CCUG 59304(T)=DSM 23328(T)=LMG 26304(T)) and Enterococcus quebecensis sp. nov. (type strain CCRI-16985(T)=CCUG 59306(T)=DSM 23327(T)=LMG 26306(T)) are proposed for the two hitherto undescribed species.},
  author       = {Sistek, Viridiana and Maheux, Andr{\'e}e F and Boissinot, Maurice and Bernard, Kathy A and Cantin, Philippe and Cleenwerck, Ilse and De Vos, Paul and Bergeron, Michel G},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {HUMAN CLINICAL SPECIMENS,Enterococcus sp. nov.,IDENTIFICATION,PCR,DNA,HYBRIDIZATION,THAILAND,GENES},
  language     = {eng},
  pages        = {1314--1320},
  title        = {Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from water},
  url          = {http://dx.doi.org/10.1099/ijs.0.029033-0},
  volume       = {62},
  year         = {2012},
}

Chicago
Sistek, Viridiana, Andrée F Maheux, Maurice Boissinot, Kathy A Bernard, Philippe Cantin, Ilse Cleenwerck, Paul De Vos, and Michel G Bergeron. 2012. “Enterococcus Ureasiticus Sp. Nov. and Enterococcus Quebecensis Sp. Nov., Isolated from Water.” International Journal of Systematic and Evolutionary Microbiology 62: 1314–1320.
APA
Sistek, V., Maheux, A. F., Boissinot, M., Bernard, K. A., Cantin, P., Cleenwerck, I., De Vos, P., et al. (2012). Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from water. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 62, 1314–1320.
Vancouver
1.
Sistek V, Maheux AF, Boissinot M, Bernard KA, Cantin P, Cleenwerck I, et al. Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from water. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2012;62:1314–20.
MLA
Sistek, Viridiana, Andrée F Maheux, Maurice Boissinot, et al. “Enterococcus Ureasiticus Sp. Nov. and Enterococcus Quebecensis Sp. Nov., Isolated from Water.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 62 (2012): 1314–1320. Print.