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Characterization of Tetragenococcus strains from sugar thick juice reveals a novel species, Tetragenococcus osmophilus sp. nov., and divides Tetragenococcus halophilus into two subspecies, T. halophilus subsp. halophilus subsp. nov. and T. halophilus subsp. flandriensis subsp. nov.

Annelies Justé, Stefanie Van Trappen UGent, Christel Verreth, Ilse Cleenwerck UGent, Paul De Vos UGent, Bart Lievens and Kris A Willems (2012) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 62(1). p.129-137
abstract
Most bacteria recovered so far from sugar thick juice during storage represent strains of the species Tetragenococcus halophilus. Recently, several Gram-positive, non-motile, non-sporeforming cocci with other physiological and genetic traits were isolated from sugar thick juice samples from different origins. In this study, representative isolates were investigated using a polyphasic taxonomic approach. The 16S rRNA gene sequence similarity between these isolates and their closest relative, Tetragenococcus muriaticus, was 97.4 %. The level of DNA–DNA relatedness between isolate T1T, representing the newly found Tetragenococcus isolates, and T. muriaticus was 57 %. Isolate T1T had a DNA G+C content of 36.7 mol%. Phylogenetic data and genomic and phenotypic features demonstrated that the isolates represent a novel species, for which the name Tetragenococcus osmophilus sp. nov. is proposed with T1T as the type strain (5LMG 26041T 5DSM 23765T). Additionally, T. halophilus isolates from high-salt and highsugar environments showed clear differences in several physiological and genetic characteristics like RAPD fingerprints and 16S rRNA gene sequences. DNA–DNA hybridizations, however, showed 79 to 80% relatedness between osmophilic and halophilic T. halophilus isolates, demonstrating that the different strains belong to the same species. Based on the phenotypic and genotypic differences observed, as well as the different origins of the strains and the industrial relevance of thick juice degradation, two subspecies of T. halophilus are described in this manuscript: T. halophilus subsp. halophilus subsp. nov. for the strains isolated from salt media and T. halophilus subsp. flandriensis subsp. nov. for the strains isolated from sugar-rich environments, which were first isolated in Flanders, Belgium. The type strains for the subspecies are IAM 1676T (5LMG 11490T 5DSM 20339T) and T5T (5LMG 26042T 5DSM 23766T), respectively.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DEOXYRIBONUCLEIC-ACID, PHYSIOLOGICAL DIVERSITY, HYBRIDIZATION, DEGRADATION, BACTERIUM
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
62
issue
1
pages
129 - 137
Web of Science type
Article
Web of Science id
000300136200023
JCR category
MICROBIOLOGY
JCR impact factor
2.112 (2012)
JCR rank
68/116 (2012)
JCR quartile
3 (2012)
ISSN
1466-5026
DOI
10.1099/ijs.0.029157-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2050921
handle
http://hdl.handle.net/1854/LU-2050921
date created
2012-02-28 21:41:52
date last changed
2012-04-03 16:20:48
@article{2050921,
  abstract     = {Most bacteria recovered so far from sugar thick juice during storage represent strains of the species Tetragenococcus halophilus. Recently, several Gram-positive, non-motile, non-sporeforming cocci with other physiological and genetic traits were isolated from sugar thick juice samples from different origins. In this study, representative isolates were investigated using a polyphasic taxonomic approach. The 16S rRNA gene sequence similarity between these isolates and their closest relative, Tetragenococcus muriaticus, was 97.4 \%. The level of DNA--DNA relatedness between isolate T1T, representing the newly found Tetragenococcus isolates, and T. muriaticus was 57 \%. Isolate T1T had a DNA G+C content of 36.7 mol\%. Phylogenetic data and genomic and phenotypic features demonstrated that the isolates represent a novel species, for which the name Tetragenococcus osmophilus sp. nov. is proposed with T1T as the type strain (5LMG 26041T 5DSM 23765T). Additionally, T. halophilus isolates from high-salt and highsugar environments showed clear differences in several physiological and genetic characteristics like RAPD fingerprints and 16S rRNA gene sequences. DNA--DNA hybridizations, however, showed 79 to 80\% relatedness between osmophilic and halophilic T. halophilus isolates, demonstrating that the different strains belong to the same species. Based on the phenotypic and genotypic differences observed, as well as the different origins of the strains and the industrial relevance of thick juice degradation, two subspecies of T. halophilus are described in this manuscript: T. halophilus subsp. halophilus subsp. nov. for the strains isolated from salt media and T. halophilus subsp. flandriensis subsp. nov. for the strains isolated from sugar-rich environments, which were first isolated in Flanders, Belgium. The type strains for the subspecies are IAM 1676T (5LMG 11490T 5DSM 20339T) and T5T (5LMG 26042T 5DSM 23766T), respectively.},
  author       = {Just{\'e}, Annelies and Van Trappen, Stefanie and Verreth, Christel and Cleenwerck, Ilse and De Vos, Paul and Lievens, Bart and Willems, Kris A},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {DEOXYRIBONUCLEIC-ACID,PHYSIOLOGICAL DIVERSITY,HYBRIDIZATION,DEGRADATION,BACTERIUM},
  language     = {eng},
  number       = {1},
  pages        = {129--137},
  title        = {Characterization of Tetragenococcus strains from sugar thick juice reveals a novel species, Tetragenococcus osmophilus sp. nov., and divides Tetragenococcus halophilus into two subspecies, T. halophilus subsp. halophilus subsp. nov. and T. halophilus subsp. flandriensis subsp. nov.},
  url          = {http://dx.doi.org/10.1099/ijs.0.029157-0},
  volume       = {62},
  year         = {2012},
}

Chicago
Justé, Annelies, Stefanie Van Trappen, Christel Verreth, Ilse Cleenwerck, Paul De Vos, Bart Lievens, and Kris A Willems. 2012. “Characterization of Tetragenococcus Strains from Sugar Thick Juice Reveals a Novel Species, Tetragenococcus Osmophilus Sp. Nov., and Divides Tetragenococcus Halophilus into Two Subspecies, T. Halophilus Subsp. Halophilus Subsp. Nov. and T. Halophilus Subsp. Flandriensis Subsp. Nov.” International Journal of Systematic and Evolutionary Microbiology 62 (1): 129–137.
APA
Justé, A., Van Trappen, S., Verreth, C., Cleenwerck, I., De Vos, P., Lievens, B., & Willems, K. A. (2012). Characterization of Tetragenococcus strains from sugar thick juice reveals a novel species, Tetragenococcus osmophilus sp. nov., and divides Tetragenococcus halophilus into two subspecies, T. halophilus subsp. halophilus subsp. nov. and T. halophilus subsp. flandriensis subsp. nov. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 62(1), 129–137.
Vancouver
1.
Justé A, Van Trappen S, Verreth C, Cleenwerck I, De Vos P, Lievens B, et al. Characterization of Tetragenococcus strains from sugar thick juice reveals a novel species, Tetragenococcus osmophilus sp. nov., and divides Tetragenococcus halophilus into two subspecies, T. halophilus subsp. halophilus subsp. nov. and T. halophilus subsp. flandriensis subsp. nov. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2012;62(1):129–37.
MLA
Justé, Annelies, Stefanie Van Trappen, Christel Verreth, et al. “Characterization of Tetragenococcus Strains from Sugar Thick Juice Reveals a Novel Species, Tetragenococcus Osmophilus Sp. Nov., and Divides Tetragenococcus Halophilus into Two Subspecies, T. Halophilus Subsp. Halophilus Subsp. Nov. and T. Halophilus Subsp. Flandriensis Subsp. Nov.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 62.1 (2012): 129–137. Print.