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Pulmonary lymphoid neogenesis in idiopathic pulmonary arterial hypertension

Frédèric Perros UGent, Peter Dorfmüller, David Montani, Hamida Hammad UGent, Wim Waelput, Barbara Girerd, Nicolas Raymond, Olaf Mercier, Sacha Mussot and Sylvia Cohen-Kaminsky, et al. (2012) AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE. 185(3). p.311-321
abstract
Rationale Patients with idiopathic pulmonary arterial hypertension (IPAH) present circulating autoantibodies against vascular wall components. Pathogenic antibodies may be generated in tertiary (ectopic) lymphoid tissues (tLTs). Objectives: To assess the frequency of tLTs in IPAH lungs, as compared with control subjects and flow-induced PAH in patients with Eisenmenger syndrome, and to identify local mechanisms responsible for their formation, perpetuation, and function. Methods: tLT composition and structure were studied by multiple immunostainings. Cytokine/chemokine and growth factor expression was quantified by real-time polymerase chain reaction and localized by immunofluorescence. The systemic mark of pulmonary lymphoid neogenesis was investigated by flow cytometry analyses of circulating lymphocytes. Measurements and Main Results: As opposed to lungs from control subjects and patients with Eisenmenger syndrome, IPAH lungs contained perivascular tLTs, comprising B- and T-cell areas with high endothelial venules and dendritic cells. Lymphocyte survival factors, such as IL-7 and platelet-derived growth factor-A, were expressed in tLTs as well as the lymphorganogenic cytokines/chemokines, lymphotoxin-alpha/-beta, CCL19, CCL20, CCL21, and CXCL13, which might explain the depletion of circulating CCR6(+) and CXCR5(+) lymphocytes. tLTs were connected with remodeled vessels via an ER-TR7(+) stromal network and supplied by lymphatic channels. The presence of germinal center centroblasts, follicular dendritic cells, activation-induced cytidine deaminase, and IL-21(+) PD1(+) follicular helper T cells in tLTs together with CD138(+) plasma cell accumulation around remodeled vessels in areas of immunoglobulin deposition argued for local immunoglobulin class switching and ongoing production. Conclusions: We highlight the main features of lymphoid neogenesis specifically in the lungs of patients with IPAH, providing new evidence of immunological mechanisms in this severe condition.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IMMUNOSUPPRESSIVE THERAPY, LOCAL IMMUNITY, T-CELLS, EXPRESSION, TISSUE, DISEASE, GROWTH, LUNG, PROLIFERATION, DENDRITIC CELL, tertiary lymphoid follicles, pulmonary arterial hypertension, lymphorganogenic chemokines, lymphatic vessel, autoantibody
journal title
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
Am. J. Respir. Crit. Care Med.
volume
185
issue
3
pages
311 - 321
Web of Science type
Article
Web of Science id
000299803000014
JCR category
RESPIRATORY SYSTEM
JCR impact factor
11.041 (2012)
JCR rank
1/50 (2012)
JCR quartile
1 (2012)
ISSN
1073-449X
DOI
10.1164/rccm.201105-0927OC
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2049913
handle
http://hdl.handle.net/1854/LU-2049913
date created
2012-02-28 17:21:50
date last changed
2015-06-17 09:58:33
@article{2049913,
  abstract     = {Rationale Patients with idiopathic pulmonary arterial hypertension (IPAH) present circulating autoantibodies against vascular wall components. Pathogenic antibodies may be generated in tertiary (ectopic) lymphoid tissues (tLTs). 
Objectives: To assess the frequency of tLTs in IPAH lungs, as compared with control subjects and flow-induced PAH in patients with Eisenmenger syndrome, and to identify local mechanisms responsible for their formation, perpetuation, and function. 
Methods: tLT composition and structure were studied by multiple immunostainings. Cytokine/chemokine and growth factor expression was quantified by real-time polymerase chain reaction and localized by immunofluorescence. The systemic mark of pulmonary lymphoid neogenesis was investigated by flow cytometry analyses of circulating lymphocytes. 
Measurements and Main Results: As opposed to lungs from control subjects and patients with Eisenmenger syndrome, IPAH lungs contained perivascular tLTs, comprising B- and T-cell areas with high endothelial venules and dendritic cells. Lymphocyte survival factors, such as IL-7 and platelet-derived growth factor-A, were expressed in tLTs as well as the lymphorganogenic cytokines/chemokines, lymphotoxin-alpha/-beta, CCL19, CCL20, CCL21, and CXCL13, which might explain the depletion of circulating CCR6(+) and CXCR5(+) lymphocytes. tLTs were connected with remodeled vessels via an ER-TR7(+) stromal network and supplied by lymphatic channels. The presence of germinal center centroblasts, follicular dendritic cells, activation-induced cytidine deaminase, and IL-21(+) PD1(+) follicular helper T cells in tLTs together with CD138(+) plasma cell accumulation around remodeled vessels in areas of immunoglobulin deposition argued for local immunoglobulin class switching and ongoing production. 
Conclusions: We highlight the main features of lymphoid neogenesis specifically in the lungs of patients with IPAH, providing new evidence of immunological mechanisms in this severe condition.},
  author       = {Perros, Fr{\'e}d{\`e}ric and Dorfm{\"u}ller, Peter and Montani, David and Hammad, Hamida and Waelput, Wim and Girerd, Barbara and Raymond, Nicolas and Mercier, Olaf and Mussot, Sacha and Cohen-Kaminsky, Sylvia and Humbert, Marc and Lambrecht, Bart},
  issn         = {1073-449X},
  journal      = {AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE},
  keyword      = {IMMUNOSUPPRESSIVE THERAPY,LOCAL IMMUNITY,T-CELLS,EXPRESSION,TISSUE,DISEASE,GROWTH,LUNG,PROLIFERATION,DENDRITIC CELL,tertiary lymphoid follicles,pulmonary arterial hypertension,lymphorganogenic chemokines,lymphatic vessel,autoantibody},
  language     = {eng},
  number       = {3},
  pages        = {311--321},
  title        = {Pulmonary lymphoid neogenesis in idiopathic pulmonary arterial hypertension},
  url          = {http://dx.doi.org/10.1164/rccm.201105-0927OC},
  volume       = {185},
  year         = {2012},
}

Chicago
Perros, Frédèric, Peter Dorfmüller, David Montani, Hamida Hammad, Wim Waelput, Barbara Girerd, Nicolas Raymond, et al. 2012. “Pulmonary Lymphoid Neogenesis in Idiopathic Pulmonary Arterial Hypertension.” American Journal of Respiratory and Critical Care Medicine 185 (3): 311–321.
APA
Perros, F., Dorfmüller, P., Montani, D., Hammad, H., Waelput, W., Girerd, B., Raymond, N., et al. (2012). Pulmonary lymphoid neogenesis in idiopathic pulmonary arterial hypertension. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 185(3), 311–321.
Vancouver
1.
Perros F, Dorfmüller P, Montani D, Hammad H, Waelput W, Girerd B, et al. Pulmonary lymphoid neogenesis in idiopathic pulmonary arterial hypertension. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE. 2012;185(3):311–21.
MLA
Perros, Frédèric, Peter Dorfmüller, David Montani, et al. “Pulmonary Lymphoid Neogenesis in Idiopathic Pulmonary Arterial Hypertension.” AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE 185.3 (2012): 311–321. Print.