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T-2 toxin induced Salmonella Typhimurium intoxication results in decreased Salmonella numbers in the cecum contents of pigs, despite marked effects on Salmonella-host cell interactions

Elin Verbrugghe UGent, Virginie Vandenbroucke UGent, Maarten Dhaenens UGent, Neil Shearer, Joline Goossens UGent, Sarah De Saeger UGent, Mia Eeckhout UGent, Katharina D'Herde UGent, Arthur Thompson and Dieter Deforce UGent, et al. (2012) VETERINARY RESEARCH. 43.
abstract
The mycotoxin T-2 toxin and Salmonella Typhimurium infections pose a significant threat to human and animal health. Interactions between both agents may result in a different outcome of the infection. Therefore, the aim of the presented study was to investigate the effects of low and relevant concentrations of T-2 toxin on the course of a Salmonella Typhimurium infection in pigs. We showed that the presence of 15 and 83 mu g T-2 toxin per kg feed significantly decreased the amount of Salmonella Typhimurium bacteria present in the cecum contents, and a tendency to a reduced colonization of the jejunum, ileum, cecum, colon and colon contents was noticed. In vitro, proteomic analysis of porcine enterocytes revealed that a very low concentration of T-2 toxin (5 ng/mL) affects the protein expression of mitochondrial, endoplasmatic reticulum and cytoskeleton associated proteins, proteins involved in protein synthesis and folding, RNA synthesis, mitogen-activated protein kinase signaling and regulatory processes. Similarly low concentrations (1-100 ng/mL) promoted the susceptibility of porcine macrophages and intestinal epithelial cells to Salmonella Typhimurium invasion, in a SPI-1 independent manner. Furthermore, T-2 toxin (1-5 ng/mL) promoted the translocation of Salmonella Typhimurium over an intestinal porcine epithelial cell monolayer. Although these findings may seem in favour of Salmonella Typhimurium, microarray analysis showed that T-2 toxin (5 ng/mL) causes an intoxication of Salmonella Typhimurium, represented by a reduced motility and a downregulation of metabolic and Salmonella Pathogenicity Island 1 genes. This study demonstrates marked interactions of T-2 toxin with Salmonella Typhimurium pathogenesis, resulting in bacterial intoxication.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
INTERLEUKIN-8 SECRETION, N-TERMINAL KINASE, PORCINE MACROPHAGES, ESCHERICHIA-COLI, EPITHELIAL-CELLS, GENE-EXPRESSION, PROTEIN-KINASE, ORAL-EXPOSURE, GROWING SWINE, CYCLE ARREST
journal title
VETERINARY RESEARCH
Vet. Res.
volume
43
article_number
22
pages
18 pages
Web of Science type
Article
Web of Science id
000304769900001
JCR category
VETERINARY SCIENCES
JCR impact factor
3.426 (2012)
JCR rank
1/142 (2012)
JCR quartile
1 (2012)
ISSN
0928-4249
DOI
10.1186/1297-9716-43-22
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2047530
handle
http://hdl.handle.net/1854/LU-2047530
date created
2012-02-27 15:16:36
date last changed
2012-09-19 16:48:13
@article{2047530,
  abstract     = {The mycotoxin T-2 toxin and Salmonella Typhimurium infections pose a significant threat to human and animal health. Interactions between both agents may result in a different outcome of the infection. Therefore, the aim of the presented study was to investigate the effects of low and relevant concentrations of T-2 toxin on the course of a Salmonella Typhimurium infection in pigs. We showed that the presence of 15 and 83 mu g T-2 toxin per kg feed significantly decreased the amount of Salmonella Typhimurium bacteria present in the cecum contents, and a tendency to a reduced colonization of the jejunum, ileum, cecum, colon and colon contents was noticed. In vitro, proteomic analysis of porcine enterocytes revealed that a very low concentration of T-2 toxin (5 ng/mL) affects the protein expression of mitochondrial, endoplasmatic reticulum and cytoskeleton associated proteins, proteins involved in protein synthesis and folding, RNA synthesis, mitogen-activated protein kinase signaling and regulatory processes. Similarly low concentrations (1-100 ng/mL) promoted the susceptibility of porcine macrophages and intestinal epithelial cells to Salmonella Typhimurium invasion, in a SPI-1 independent manner. Furthermore, T-2 toxin (1-5 ng/mL) promoted the translocation of Salmonella Typhimurium over an intestinal porcine epithelial cell monolayer. Although these findings may seem in favour of Salmonella Typhimurium, microarray analysis showed that T-2 toxin (5 ng/mL) causes an intoxication of Salmonella Typhimurium, represented by a reduced motility and a downregulation of metabolic and Salmonella Pathogenicity Island 1 genes. This study demonstrates marked interactions of T-2 toxin with Salmonella Typhimurium pathogenesis, resulting in bacterial intoxication.},
  articleno    = {22},
  author       = {Verbrugghe, Elin and Vandenbroucke, Virginie and Dhaenens, Maarten and Shearer, Neil and Goossens, Joline and De Saeger, Sarah and Eeckhout, Mia and D'Herde, Katharina and Thompson, Arthur and Deforce, Dieter and Boyen, Filip and Leyman, Bregje and Van Parys, Alexander and De Backer, Patrick and Haesebrouck, Freddy and Croubels, Siska and Pasmans, Frank},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {INTERLEUKIN-8 SECRETION,N-TERMINAL KINASE,PORCINE MACROPHAGES,ESCHERICHIA-COLI,EPITHELIAL-CELLS,GENE-EXPRESSION,PROTEIN-KINASE,ORAL-EXPOSURE,GROWING SWINE,CYCLE ARREST},
  language     = {eng},
  pages        = {18},
  title        = {T-2 toxin induced Salmonella Typhimurium intoxication results in decreased Salmonella numbers in the cecum contents of pigs, despite marked effects on Salmonella-host cell interactions},
  url          = {http://dx.doi.org/10.1186/1297-9716-43-22},
  volume       = {43},
  year         = {2012},
}

Chicago
Verbrugghe, Elin, Virginie Vandenbroucke, Maarten Dhaenens, Neil Shearer, Joline Goossens, Sarah De Saeger, Mia Eeckhout, et al. 2012. “T-2 Toxin Induced Salmonella Typhimurium Intoxication Results in Decreased Salmonella Numbers in the Cecum Contents of Pigs, Despite Marked Effects on Salmonella-host Cell Interactions.” Veterinary Research 43.
APA
Verbrugghe, E., Vandenbroucke, V., Dhaenens, M., Shearer, N., Goossens, J., De Saeger, S., Eeckhout, M., et al. (2012). T-2 toxin induced Salmonella Typhimurium intoxication results in decreased Salmonella numbers in the cecum contents of pigs, despite marked effects on Salmonella-host cell interactions. VETERINARY RESEARCH, 43.
Vancouver
1.
Verbrugghe E, Vandenbroucke V, Dhaenens M, Shearer N, Goossens J, De Saeger S, et al. T-2 toxin induced Salmonella Typhimurium intoxication results in decreased Salmonella numbers in the cecum contents of pigs, despite marked effects on Salmonella-host cell interactions. VETERINARY RESEARCH. 2012;43.
MLA
Verbrugghe, Elin, Virginie Vandenbroucke, Maarten Dhaenens, et al. “T-2 Toxin Induced Salmonella Typhimurium Intoxication Results in Decreased Salmonella Numbers in the Cecum Contents of Pigs, Despite Marked Effects on Salmonella-host Cell Interactions.” VETERINARY RESEARCH 43 (2012): n. pag. Print.