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A pilot comparison of laser-assisted vs piezo drill ICSI for the in vitro production of horse embryos

Katrien Smits UGent, Jan Govaere UGent, Maarten Hoogewijs UGent, Sofie Piepers UGent and Ann Van Soom UGent (2012) REPRODUCTION IN DOMESTIC ANIMALS. 47(1). p.e1-e3
abstract
Intracytoplasmic sperm injection (ICSI) is the method of choice for the in vitro production (IVP) of equine embryos. However, conventional ICSI has been associated with mechanical damage to the oocyte caused by the deformation of the zona pellucida (ZP) and exposure of the oolemma to negative pressure during injection. Introduction of the less traumatic and more efficient piezo drill-assisted ICSI (PDAI) yielded higher cleavage rates and more consistent results. Nevertheless, PDAI is also associated with disadvantages such as the use of mercury and possible DNA damage. This led us to explore an alternative method avoiding oocyte trauma, namely laser-assisted ICSI (LAI), which involves creating a hole in the ZP prior to ICSI. In this pilot study, PDAI and LAI were compared for ICSI in the horse. No significant influences on subsequent embryonic development were observed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
OOCYTES
journal title
REPRODUCTION IN DOMESTIC ANIMALS
Reprod. Domest. Anim.
volume
47
issue
1
pages
e1 - e3
Web of Science type
Article
Web of Science id
000298956400001
JCR category
AGRICULTURE, DAIRY & ANIMAL SCIENCE
JCR impact factor
1.392 (2012)
JCR rank
13/54 (2012)
JCR quartile
1 (2012)
ISSN
0936-6768
DOI
10.1111/j.1439-0531.2011.01814.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2046396
handle
http://hdl.handle.net/1854/LU-2046396
date created
2012-02-27 11:16:25
date last changed
2012-03-08 16:32:33
@article{2046396,
  abstract     = {Intracytoplasmic sperm injection (ICSI) is the method of choice for the in vitro production (IVP) of equine embryos. However, conventional ICSI has been associated with mechanical damage to the oocyte caused by the deformation of the zona pellucida (ZP) and exposure of the oolemma to negative pressure during injection. Introduction of the less traumatic and more efficient piezo drill-assisted ICSI (PDAI) yielded higher cleavage rates and more consistent results. Nevertheless, PDAI is also associated with disadvantages such as the use of mercury and possible DNA damage. This led us to explore an alternative method avoiding oocyte trauma, namely laser-assisted ICSI (LAI), which involves creating a hole in the ZP prior to ICSI. In this pilot study, PDAI and LAI were compared for ICSI in the horse. No significant influences on subsequent embryonic development were observed.},
  author       = {Smits, Katrien and Govaere, Jan and Hoogewijs, Maarten and Piepers, Sofie and Van Soom, Ann},
  issn         = {0936-6768},
  journal      = {REPRODUCTION IN DOMESTIC ANIMALS},
  keyword      = {OOCYTES},
  language     = {eng},
  number       = {1},
  pages        = {e1--e3},
  title        = {A pilot comparison of laser-assisted vs piezo drill ICSI for the in vitro production of horse embryos},
  url          = {http://dx.doi.org/10.1111/j.1439-0531.2011.01814.x},
  volume       = {47},
  year         = {2012},
}

Chicago
Smits, Katrien, Jan Govaere, Maarten Hoogewijs, Sofie Piepers, and Ann Van Soom. 2012. “A Pilot Comparison of Laser-assisted Vs Piezo Drill ICSI for the in Vitro Production of Horse Embryos.” Reproduction in Domestic Animals 47 (1): e1–e3.
APA
Smits, Katrien, Govaere, J., Hoogewijs, M., Piepers, S., & Van Soom, A. (2012). A pilot comparison of laser-assisted vs piezo drill ICSI for the in vitro production of horse embryos. REPRODUCTION IN DOMESTIC ANIMALS, 47(1), e1–e3.
Vancouver
1.
Smits K, Govaere J, Hoogewijs M, Piepers S, Van Soom A. A pilot comparison of laser-assisted vs piezo drill ICSI for the in vitro production of horse embryos. REPRODUCTION IN DOMESTIC ANIMALS. 2012;47(1):e1–e3.
MLA
Smits, Katrien, Jan Govaere, Maarten Hoogewijs, et al. “A Pilot Comparison of Laser-assisted Vs Piezo Drill ICSI for the in Vitro Production of Horse Embryos.” REPRODUCTION IN DOMESTIC ANIMALS 47.1 (2012): e1–e3. Print.