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A novel sensor to map auxin response and distribution at high spatio-temporal resolution

Geraldine Brunoud, Darren M Wells, Marina Oliva, Antoine Larrieu, Vincent Mirabet, Amy H Burrow, Tom Beeckman UGent, Stefan Kepinski, Jan Traas and Malcolm J Bennett, et al. (2012) NATURE. 482(7383). p.103-106
abstract
Auxin is a key plant morphogenetic signal(1) but tools to analyse dynamically its distribution and signalling during development are still limited. Auxin perception directly triggers the degradation of Aux/IAA repressor proteins(2-6). Here we describe a novel Aux/IAA-based auxin signalling sensor termed DII-VENUS that was engineered in the model plant Arabidopsis thaliana. The VENUS fast maturing form of yellow fluorescent protein(7) was fused in-frame to the Aux/IAA auxin-interaction domain (termed domain II; DII)(5) and expressed under a constitutive promoter. We initially show that DII-VENUS abundance is dependent on auxin, its TIR1/AFBs co-receptors(4-6,8) and proteasome activities. Next, we demonstrate that DII-VENUS provides a map of relative auxin distribution at cellular resolution in different tissues. DII-VENUS is also rapidly degraded in response to auxin and we used it to visualize dynamic changes in cellular auxin distribution successfully during two developmental responses, the root gravitropic response and lateral organ production at the shoot apex. Our results illustrate the value of developing response input sensors such as DII-VENUS to provide high-resolution spatio-temporal information about hormone distribution and response during plant growth and development.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BOX PROTEIN TIR1, AUX/IAA PROTEINS, ARABIDOPSIS ROOT, PLANT DEVELOPMENT, GENE-EXPRESSION, TRANSPORT, MERISTEM, RECEPTOR, GROWTH, DEGRADATION
journal title
NATURE
Nature
volume
482
issue
7383
pages
103 - 106
Web of Science type
Article
Web of Science id
000299726000044
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
38.597 (2012)
JCR rank
1/56 (2012)
JCR quartile
1 (2012)
ISSN
0028-0836
DOI
10.1038/nature10791
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2042335
handle
http://hdl.handle.net/1854/LU-2042335
date created
2012-02-23 13:50:26
date last changed
2012-04-02 13:22:05
@article{2042335,
  abstract     = {Auxin is a key plant morphogenetic signal(1) but tools to analyse dynamically its distribution and signalling during development are still limited. Auxin perception directly triggers the degradation of Aux/IAA repressor proteins(2-6). Here we describe a novel Aux/IAA-based auxin signalling sensor termed DII-VENUS that was engineered in the model plant Arabidopsis thaliana. The VENUS fast maturing form of yellow fluorescent protein(7) was fused in-frame to the Aux/IAA auxin-interaction domain (termed domain II; DII)(5) and expressed under a constitutive promoter. We initially show that DII-VENUS abundance is dependent on auxin, its TIR1/AFBs co-receptors(4-6,8) and proteasome activities. Next, we demonstrate that DII-VENUS provides a map of relative auxin distribution at cellular resolution in different tissues. DII-VENUS is also rapidly degraded in response to auxin and we used it to visualize dynamic changes in cellular auxin distribution successfully during two developmental responses, the root gravitropic response and lateral organ production at the shoot apex. Our results illustrate the value of developing response input sensors such as DII-VENUS to provide high-resolution spatio-temporal information about hormone distribution and response during plant growth and development.},
  author       = {Brunoud, Geraldine and Wells, Darren M and Oliva, Marina and Larrieu, Antoine and Mirabet, Vincent and Burrow, Amy H and Beeckman, Tom and Kepinski, Stefan and Traas, Jan and Bennett, Malcolm J and Vernoux, Teva},
  issn         = {0028-0836},
  journal      = {NATURE},
  keyword      = {BOX PROTEIN TIR1,AUX/IAA PROTEINS,ARABIDOPSIS ROOT,PLANT DEVELOPMENT,GENE-EXPRESSION,TRANSPORT,MERISTEM,RECEPTOR,GROWTH,DEGRADATION},
  language     = {eng},
  number       = {7383},
  pages        = {103--106},
  title        = {A novel sensor to map auxin response and distribution at high spatio-temporal resolution},
  url          = {http://dx.doi.org/10.1038/nature10791},
  volume       = {482},
  year         = {2012},
}

Chicago
Brunoud, Geraldine, Darren M Wells, Marina Oliva, Antoine Larrieu, Vincent Mirabet, Amy H Burrow, Tom Beeckman, et al. 2012. “A Novel Sensor to Map Auxin Response and Distribution at High Spatio-temporal Resolution.” Nature 482 (7383): 103–106.
APA
Brunoud, G., Wells, D. M., Oliva, M., Larrieu, A., Mirabet, V., Burrow, A. H., Beeckman, T., et al. (2012). A novel sensor to map auxin response and distribution at high spatio-temporal resolution. NATURE, 482(7383), 103–106.
Vancouver
1.
Brunoud G, Wells DM, Oliva M, Larrieu A, Mirabet V, Burrow AH, et al. A novel sensor to map auxin response and distribution at high spatio-temporal resolution. NATURE. 2012;482(7383):103–6.
MLA
Brunoud, Geraldine, Darren M Wells, Marina Oliva, et al. “A Novel Sensor to Map Auxin Response and Distribution at High Spatio-temporal Resolution.” NATURE 482.7383 (2012): 103–106. Print.