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Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by temporal inhibition of the anaphase-promoting complex/cyclosome

Jefri Heyman (UGent) , Hilde Van Den Daele (UGent) , Kevin De Wit, Véronique Boudolf (UGent) , Barbara Berckmans (UGent) , Aurine Verkest (UGent) , Claire Lessa Alvim Kamei (UGent) , Geert De Jaeger (UGent) , Csaba Koncz and Lieven De Veylder (UGent)
(2011) PLANT CELL. 23(12). p.4394-4410
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Abstract
The anaphase-promoting complex/cyclosome (APC/C) is a multisubunit ubiquitin ligase that regulates progression through the cell cycle by marking key cell division proteins for destruction. To ensure correct cell cycle progression, accurate timing of APC/C activity is important, which is obtained through its association with both activating and inhibitory subunits. However, although the APC/C is highly conserved among eukaryotes, no APC/C inhibitors are known in plants. Recently, we have identified ULTRAVIOLET-B-INSENSITIVE4 (UVI4) as a plant-specific component of the APC/C. Here, we demonstrate that UVI4 uses conserved APC/C interaction motifs to counteract the activity of the CELL CYCLE SWITCH52 A1 (CCS52A1) activator subunit, inhibiting the turnover of the A-type cyclin CYCA2;3. UVI4 is expressed in an S phase-dependent fashion, likely through the action of E2F transcription factors. Correspondingly, uvi4 mutant plants failed to accumulate CYCA2; 3 during the S phase and prematurely exited the cell cycle, triggering the onset of the endocycle. We conclude that UVI4 regulates the temporal inactivation of APC/C during DNA replication, allowing CYCA2;3 to accumulate above the level required for entering mitosis, and thereby regulates the meristem size and plant growth rate.
Keywords
TRANSCRIPTIONAL REGULATION, ENDO-REDUPLICATION, DNA-REPLICATION, ENDOCYCLE ONSET, GENE-EXPRESSION, THALIANA ROOT, KEN-BOX, MEIOSIS-I, COMPLEX, CYCLE

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Chicago
Heyman, Jefri, Hilde Van Den Daele, Kevin De Wit, Véronique Boudolf, Barbara Berckmans, Aurine Verkest, Claire Lessa Alvim Kamei, Geert De Jaeger, Csaba Koncz, and Lieven De Veylder. 2011. “Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 Maintains Cell Division Activity by Temporal Inhibition of the Anaphase-promoting Complex/cyclosome.” Plant Cell 23 (12): 4394–4410.
APA
Heyman, J., Van Den Daele, H., De Wit, K., Boudolf, V., Berckmans, B., Verkest, A., Lessa Alvim Kamei, C., et al. (2011). Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by temporal inhibition of the anaphase-promoting complex/cyclosome. PLANT CELL, 23(12), 4394–4410.
Vancouver
1.
Heyman J, Van Den Daele H, De Wit K, Boudolf V, Berckmans B, Verkest A, et al. Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by temporal inhibition of the anaphase-promoting complex/cyclosome. PLANT CELL. 2011;23(12):4394–410.
MLA
Heyman, Jefri, Hilde Van Den Daele, Kevin De Wit, et al. “Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 Maintains Cell Division Activity by Temporal Inhibition of the Anaphase-promoting Complex/cyclosome.” PLANT CELL 23.12 (2011): 4394–4410. Print.
@article{2042270,
  abstract     = {The anaphase-promoting complex/cyclosome (APC/C) is a multisubunit ubiquitin ligase that regulates progression through the cell cycle by marking key cell division proteins for destruction. To ensure correct cell cycle progression, accurate timing of APC/C activity is important, which is obtained through its association with both activating and inhibitory subunits. However, although the APC/C is highly conserved among eukaryotes, no APC/C inhibitors are known in plants. Recently, we have identified ULTRAVIOLET-B-INSENSITIVE4 (UVI4) as a plant-specific component of the APC/C. Here, we demonstrate that UVI4 uses conserved APC/C interaction motifs to counteract the activity of the CELL CYCLE SWITCH52 A1 (CCS52A1) activator subunit, inhibiting the turnover of the A-type cyclin CYCA2;3. UVI4 is expressed in an S phase-dependent fashion, likely through the action of E2F transcription factors. Correspondingly, uvi4 mutant plants failed to accumulate CYCA2; 3 during the S phase and prematurely exited the cell cycle, triggering the onset of the endocycle. We conclude that UVI4 regulates the temporal inactivation of APC/C during DNA replication, allowing CYCA2;3 to accumulate above the level required for entering mitosis, and thereby regulates the meristem size and plant growth rate.},
  author       = {Heyman, Jefri and Van Den Daele, Hilde and De Wit, Kevin and Boudolf, V{\'e}ronique and Berckmans, Barbara and Verkest, Aurine and Lessa Alvim Kamei, Claire and De Jaeger, Geert and Koncz, Csaba and De Veylder, Lieven},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {TRANSCRIPTIONAL REGULATION,ENDO-REDUPLICATION,DNA-REPLICATION,ENDOCYCLE ONSET,GENE-EXPRESSION,THALIANA ROOT,KEN-BOX,MEIOSIS-I,COMPLEX,CYCLE},
  language     = {eng},
  number       = {12},
  pages        = {4394--4410},
  title        = {Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by temporal inhibition of the anaphase-promoting complex/cyclosome},
  url          = {http://dx.doi.org/10.1105/tpc.111.091793},
  volume       = {23},
  year         = {2011},
}
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